Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   PSR56_RS23360 Genome accession   NZ_CP117689
Coordinates   4435660..4438002 (-) Length   780 a.a.
NCBI ID   WP_048019725.1    Uniprot ID   -
Organism   Priestia megaterium strain GEB3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4430660..4443002
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSR56_RS23335 (PSR56_23335) - 4431053..4431229 (-) 177 WP_013059305.1 hypothetical protein -
  PSR56_RS23340 (PSR56_23340) - 4431396..4432280 (+) 885 WP_231099272.1 N-acetylmuramoyl-L-alanine amidase -
  PSR56_RS23345 (PSR56_23345) dtd 4432271..4432732 (-) 462 WP_310884108.1 D-aminoacyl-tRNA deacylase -
  PSR56_RS23350 (PSR56_23350) - 4432748..4434946 (-) 2199 WP_013059308.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  PSR56_RS23355 (PSR56_23355) - 4435132..4435644 (-) 513 WP_013059309.1 adenine phosphoribosyltransferase -
  PSR56_RS23360 (PSR56_23360) recJ 4435660..4438002 (-) 2343 WP_048019725.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  PSR56_RS23365 (PSR56_23365) secDF 4438143..4440380 (-) 2238 WP_047749810.1 protein translocase subunit SecDF -
  PSR56_RS23370 (PSR56_23370) - 4440518..4440811 (-) 294 WP_028411692.1 post-transcriptional regulator -
  PSR56_RS23375 (PSR56_23375) spoVB 4440876..4442486 (+) 1611 WP_028411693.1 stage V sporulation protein B -

Sequence

Protein


Download         Length: 780 a.a.        Molecular weight: 87900.46 Da        Isoelectric Point: 4.7737

>NTDB_id=787002 PSR56_RS23360 WP_048019725.1 4435660..4438002(-) (recJ) [Priestia megaterium strain GEB3]
MLQSKKRWNVQECDEQLVNEFVENLNITPLVASLLLNRGMTTVEAAREFLHVDAQTFHDPFLLHDMDKAVARIQQAIDHN
EKIMIYGDYDADGVSSTSVMLSALQQLGADTDFYIPNRFTEGYGPNKAAFDKINAGGYQLVITVDTGISAVEETAYAREL
DFDLIITDHHEPGPVLPDAYAIIHPKLPGSTYPFKELAGVGVAFKLAHALLGELPEHLLELAVIGTIADLVPLVDENRLI
ARKGLDYLKLTKRTGIEALLSVCGVKREEITEDTIGFAMAPRINAVGRLQDADPAVHLLMTTNAEEAKGLAKEIDALNKE
RQQIVNDITEEAVEFVESLYPPDENSVLIVEGEGWNAGVVGIVASRLVEKFYRPTIVLSVDSENHIAKGSARSIQGFDLF
ANLSKCRDILPHFGGHPMAAGMTLNSEDVEELRRRLNEQASNVLTQQDFIPITSVDTVCKTEEITLENIEQMSALAPFGM
HNPKPKVLIKDVHTTSMRKIGANKNHLKLVFEDSGVSVDAVGFGLGYILDEVSPIAKVSLVGELSINEWNNMRKPQLMVE
DISVDEWQMFDYRSAVDVKRFFEQVFTDQMKVVAFHDSTIDKYKSMLPMEHVVLIQNEEQAKACMFAGEQIVLLDLPPSQ
KWLEALFVQGIPSRIYAIFLTEEDHYFDTIPTRDHFKWFYGFLAKKGPFDLKRYKEDLAIHKGWSKETIDFMAKVFFELE
FVTINNGLISLVKNSTKRDLIESATYRQKQQQIEIEKNFIYTSYVQLKNQFQDIFKRSIE

Nucleotide


Download         Length: 2343 bp        

>NTDB_id=787002 PSR56_RS23360 WP_048019725.1 4435660..4438002(-) (recJ) [Priestia megaterium strain GEB3]
ATGTTACAGTCAAAAAAACGTTGGAATGTTCAAGAATGTGATGAACAACTTGTTAATGAATTTGTAGAAAACCTCAATAT
TACACCTCTAGTAGCTTCGTTATTACTTAATAGAGGCATGACAACAGTAGAAGCGGCACGTGAGTTTTTACACGTTGATG
CACAAACGTTCCACGATCCTTTTTTATTGCACGATATGGACAAAGCAGTAGCTCGTATTCAGCAAGCAATTGATCATAAT
GAAAAAATTATGATCTATGGAGATTACGATGCGGACGGCGTCAGCAGTACTTCAGTTATGTTATCTGCTTTACAGCAGCT
CGGCGCTGATACTGATTTTTATATTCCTAATCGCTTTACAGAAGGTTATGGGCCGAATAAGGCAGCATTTGATAAAATAA
ACGCAGGCGGCTATCAGCTGGTGATTACGGTAGACACAGGTATTTCGGCAGTCGAAGAAACGGCGTATGCACGTGAATTA
GATTTTGATTTAATTATTACTGATCACCACGAGCCGGGTCCGGTTTTACCCGATGCGTATGCAATTATTCATCCAAAGCT
TCCGGGAAGTACATATCCATTTAAAGAACTGGCAGGTGTAGGAGTGGCTTTTAAACTCGCTCATGCGCTGTTAGGGGAGC
TTCCGGAACATTTGTTAGAACTAGCAGTGATTGGGACGATTGCTGACTTAGTTCCTTTAGTCGACGAAAACCGCTTAATT
GCTCGCAAAGGTCTTGACTATCTCAAGCTCACGAAGCGTACTGGAATTGAAGCGCTGCTGAGCGTATGTGGAGTAAAACG
CGAAGAAATTACTGAAGATACAATTGGATTTGCCATGGCTCCTCGCATTAATGCAGTAGGACGTCTTCAAGATGCTGATC
CAGCTGTTCATTTGCTTATGACAACAAATGCTGAAGAAGCAAAAGGTTTAGCTAAAGAAATTGATGCGTTAAACAAGGAA
AGGCAGCAAATCGTAAATGATATTACGGAAGAAGCCGTTGAATTTGTTGAAAGTTTGTATCCTCCGGATGAAAATAGCGT
TCTTATTGTAGAGGGAGAAGGATGGAATGCAGGCGTTGTTGGAATTGTTGCTTCTAGGCTCGTAGAAAAGTTCTACAGAC
CGACAATTGTATTAAGTGTTGACTCTGAAAACCATATTGCTAAAGGTTCAGCAAGAAGTATCCAAGGCTTTGACTTATTC
GCGAACTTATCTAAATGCCGTGACATTTTGCCGCATTTTGGAGGGCATCCAATGGCAGCCGGCATGACGTTAAACAGCGA
GGATGTAGAAGAACTGCGCAGACGCCTTAATGAACAAGCAAGCAACGTGTTAACTCAACAGGACTTTATTCCAATTACAT
CAGTTGATACAGTATGCAAAACAGAGGAAATTACGCTGGAAAACATTGAACAAATGAGTGCGCTTGCTCCATTTGGTATG
CATAATCCGAAGCCAAAAGTTCTCATTAAAGATGTTCATACAACATCTATGCGGAAAATTGGCGCGAATAAAAATCATTT
AAAACTCGTATTTGAAGACAGCGGTGTATCGGTTGATGCAGTAGGATTTGGGTTAGGCTATATATTAGATGAAGTATCTC
CTATAGCTAAGGTATCATTAGTAGGTGAACTATCTATTAATGAGTGGAACAATATGCGCAAACCGCAGCTGATGGTAGAA
GATATCTCTGTAGATGAGTGGCAGATGTTTGATTATCGCAGCGCGGTTGATGTCAAACGTTTCTTTGAACAGGTATTCAC
AGATCAAATGAAAGTTGTTGCTTTTCACGATTCAACAATTGATAAATACAAGTCGATGCTGCCGATGGAACATGTTGTTC
TTATTCAAAATGAAGAACAGGCAAAAGCATGCATGTTTGCAGGAGAACAGATCGTTTTATTAGATTTGCCTCCGTCACAG
AAGTGGCTTGAAGCTTTGTTTGTCCAAGGTATTCCTTCTAGGATATACGCTATTTTCTTAACGGAAGAAGACCATTATTT
TGATACAATCCCAACTCGCGATCACTTTAAATGGTTCTACGGCTTTTTAGCGAAAAAGGGTCCTTTTGATTTAAAACGCT
ACAAAGAAGATTTAGCAATTCATAAGGGCTGGTCGAAAGAAACGATTGATTTTATGGCAAAGGTGTTTTTTGAGTTAGAA
TTTGTTACAATAAACAATGGCTTAATTTCGTTAGTGAAAAATTCTACGAAACGTGATTTAATAGAATCTGCGACTTATCG
CCAAAAGCAGCAGCAGATTGAAATAGAAAAAAACTTTATTTATACGTCATATGTACAGCTTAAAAATCAATTTCAAGATA
TTTTTAAGCGTTCGATTGAATGA

Domains

Predicted by InterproScan.

DHHA1 domain (DHHA1)

(306-439)

DHH family (DHH)

(82-226)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-770)

RecJ OB domain (RecJ_OB)

(455-561)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

55.385

100

0.554