Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   Q5R37_RS22125 Genome accession   NZ_CP130706
Coordinates   4559634..4560398 (+) Length   254 a.a.
NCBI ID   WP_001136229.1    Uniprot ID   B7L5T2
Organism   Escherichia coli strain DDG220     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 4554634..4565398
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q5R37_RS22105 (Q5R37_22105) acpT 4555588..4556175 (+) 588 WP_000285774.1 4'-phosphopantetheinyl transferase AcpT -
  Q5R37_RS22110 (Q5R37_22110) nikA 4556286..4557860 (+) 1575 WP_000953361.1 nickel ABC transporter substrate-binding protein -
  Q5R37_RS22115 (Q5R37_22115) nikB 4557860..4558804 (+) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  Q5R37_RS22120 (Q5R37_22120) nikC 4558801..4559634 (+) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  Q5R37_RS22125 (Q5R37_22125) amiE 4559634..4560398 (+) 765 WP_001136229.1 nickel import ATP-binding protein NikD Regulator
  Q5R37_RS22130 (Q5R37_22130) nikE 4560395..4561201 (+) 807 WP_000173631.1 nickel import ATP-binding protein NikE -
  Q5R37_RS22135 (Q5R37_22135) nikR 4561207..4561608 (+) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26820.38 Da        Isoelectric Point: 6.3761

>NTDB_id=786735 Q5R37_RS22125 WP_001136229.1 4559634..4560398(+) (amiE) [Escherichia coli strain DDG220]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=786735 Q5R37_RS22125 WP_001136229.1 4559634..4560398(+) (amiE) [Escherichia coli strain DDG220]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTTGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCTGACGGTAAGATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACAGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7L5T2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398