Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilR   Type   Regulator
Locus tag   PSH97_RS23935 Genome accession   NZ_CP117454
Coordinates   5278212..5279552 (+) Length   446 a.a.
NCBI ID   WP_305446951.1    Uniprot ID   -
Organism   Pseudomonas cucumis strain FP1935     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5273212..5284552
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH97_RS23910 (PSH97_23905) pgeF 5273401..5274126 (-) 726 WP_305446947.1 peptidoglycan editing factor PgeF -
  PSH97_RS23915 (PSH97_23910) rluD 5274123..5275085 (-) 963 WP_007901932.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  PSH97_RS23920 (PSH97_23915) - 5275238..5276260 (+) 1023 WP_305446948.1 outer membrane protein assembly factor BamD -
  PSH97_RS23925 (PSH97_23920) - 5276397..5276630 (+) 234 WP_305446949.1 PP0621 family protein -
  PSH97_RS23930 (PSH97_23925) pilS 5276620..5278209 (+) 1590 WP_305446950.1 PAS domain-containing sensor histidine kinase Regulator
  PSH97_RS23935 (PSH97_23930) pilR 5278212..5279552 (+) 1341 WP_305446951.1 sigma-54 dependent transcriptional regulator Regulator
  PSH97_RS23940 (PSH97_23935) thiO 5279610..5280710 (-) 1101 WP_305446952.1 glycine oxidase ThiO -
  PSH97_RS23945 (PSH97_23940) - 5280767..5281168 (-) 402 WP_305446953.1 type IV pilin protein -
  PSH97_RS23950 (PSH97_23945) - 5281183..5284305 (-) 3123 WP_305446954.1 pilus assembly protein -

Sequence

Protein


Download         Length: 446 a.a.        Molecular weight: 49602.96 Da        Isoelectric Point: 6.5135

>NTDB_id=785545 PSH97_RS23935 WP_305446951.1 5278212..5279552(+) (pilR) [Pseudomonas cucumis strain FP1935]
MSPRQKILIVDDEPDIRELLEITLGRMKLDTFSARNLAEAQALLARETFDLCLTDMRLPDGTGLALVQHIQQCYAQVPVA
MITAYGSLETAINALKAGAFDFLTKPVDLTRLRELVTSALSLPAPGGASSAIDRRLLGDSLPMRSLRKQIDKLARSQAPV
YISGESGSGKELVARLIHDQGPRATRPFVPVNCGAIPSELMESEFFGHRKGSFSGAIEDKPGLFQAAQGGTLFLDEVADL
PLAMQVKLLRAIQEKAVRSIGGQQETVVDVRILCATHKDLDTEVAAERFRQDLYYRLNVIELRVPPLRERRDDIELLANN
MLKRLATGTGQPAAKLHPQALDALKSYRFPGNVRELENMLERAHTLCENKQIEASDLRLVEGNGNSDSDVPDLTQIDNLE
AYLENVERKLILQALEETRWNRTAAAQRLNLSFRSMRYRLKKLGLD

Nucleotide


Download         Length: 1341 bp        

>NTDB_id=785545 PSH97_RS23935 WP_305446951.1 5278212..5279552(+) (pilR) [Pseudomonas cucumis strain FP1935]
ATGAGCCCACGGCAAAAAATCCTGATCGTCGACGACGAGCCGGATATCCGCGAACTCCTGGAAATCACCCTAGGACGGAT
GAAACTCGACACCTTCAGTGCCCGCAACCTTGCAGAAGCACAGGCGCTGCTGGCACGGGAGACGTTCGACCTGTGCCTGA
CCGACATGCGCCTGCCCGACGGCACCGGTCTGGCGCTGGTGCAGCACATCCAGCAGTGCTATGCGCAAGTGCCAGTAGCG
ATGATCACTGCCTACGGTAGCCTGGAGACGGCAATCAACGCGCTCAAGGCTGGCGCCTTCGATTTTTTGACCAAACCGGT
TGACCTGACCCGCTTGCGCGAACTGGTCACCAGCGCCTTGAGTCTGCCGGCACCAGGCGGTGCCAGCAGCGCCATCGACC
GTCGGCTACTGGGCGACTCCCTGCCGATGCGCAGCCTGCGCAAACAAATCGACAAACTGGCCCGCAGCCAGGCTCCGGTA
TACATCAGCGGTGAGTCCGGCAGCGGCAAGGAGTTGGTCGCCCGACTGATCCACGACCAAGGCCCACGCGCCACCCGACC
GTTCGTGCCGGTGAATTGTGGGGCTATCCCGTCGGAATTGATGGAAAGCGAGTTTTTCGGCCATCGCAAAGGCAGTTTCA
GCGGAGCCATCGAAGACAAACCCGGGCTGTTCCAGGCCGCTCAGGGTGGCACTTTGTTCCTCGATGAAGTGGCAGATCTG
CCGCTGGCAATGCAGGTCAAACTTTTGCGGGCCATTCAAGAGAAAGCCGTGCGCAGCATCGGTGGCCAGCAGGAAACAGT
GGTCGATGTGCGCATACTCTGTGCCACCCACAAAGACCTCGATACCGAAGTCGCCGCCGAACGCTTTCGCCAGGACTTGT
ACTACCGTCTGAATGTGATCGAGCTGCGGGTGCCGCCCTTGCGCGAACGCCGCGACGATATTGAGCTTCTGGCCAACAAT
ATGCTCAAGCGACTGGCGACTGGCACTGGCCAACCCGCTGCAAAACTCCACCCGCAAGCCCTCGACGCACTGAAAAGCTA
TCGTTTTCCAGGAAACGTGCGGGAATTGGAGAACATGCTTGAGCGGGCGCACACCTTGTGCGAGAACAAACAAATCGAAG
CCAGCGACCTACGGTTGGTGGAAGGTAACGGCAATTCGGACAGCGACGTGCCGGACCTGACGCAGATCGACAATCTGGAA
GCGTATCTGGAAAATGTCGAACGCAAACTCATCCTCCAGGCCCTGGAAGAAACCCGCTGGAACCGCACGGCAGCGGCTCA
GCGGTTGAATCTGTCGTTTCGGTCGATGCGTTATCGACTGAAGAAGTTGGGGTTGGATTGA

Domains

Predicted by InterproScan.

Response regulator receiver domain (Response_reg)

(7-116)

Sigma-54 interaction domain (Sigma54_activat)

(137-302)

Bacterial regulatory protein, Fis family (HTH_8)

(403-443)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilR Pseudomonas aeruginosa PAK

79.007

99.327

0.785
  pilR Acinetobacter baumannii strain A118

48.366

100

0.498