Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PQQ10_RS03705 Genome accession   NZ_CP117351
Coordinates   770045..771025 (+) Length   326 a.a.
NCBI ID   WP_274896048.1    Uniprot ID   -
Organism   Salmonella enterica subsp. enterica serovar Dublin strain RM100     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 765045..776025
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PQQ10_RS03675 (PQQ10_03675) - 765620..766627 (+) 1008 WP_000252201.1 DUF1202 family protein -
  PQQ10_RS03680 (PQQ10_03680) hemW 766721..767857 (-) 1137 WP_001096516.1 radical SAM family heme chaperone HemW -
  PQQ10_RS03685 (PQQ10_03685) - 767850..768443 (-) 594 WP_001174769.1 XTP/dITP diphosphatase -
  PQQ10_RS03690 (PQQ10_03690) yggU 768451..768741 (-) 291 WP_001277205.1 DUF167 family protein YggU -
  PQQ10_RS03695 (PQQ10_03695) - 768738..769304 (-) 567 WP_001094850.1 YggT family protein -
  PQQ10_RS03700 (PQQ10_03700) - 769323..770027 (-) 705 WP_000997812.1 YggS family pyridoxal phosphate-dependent enzyme -
  PQQ10_RS03705 (PQQ10_03705) pilT 770045..771025 (+) 981 WP_274896048.1 type IV pilus twitching motility protein PilT Machinery gene
  PQQ10_RS03710 (PQQ10_03710) - 771158..771844 (+) 687 WP_000098324.1 IclR family transcriptional regulator -
  PQQ10_RS03715 (PQQ10_03715) ruvX 771891..772307 (-) 417 WP_001285490.1 Holliday junction resolvase RuvX -
  PQQ10_RS03720 (PQQ10_03720) - 772307..772870 (-) 564 WP_001053173.1 YqgE/AlgH family protein -
  PQQ10_RS03725 (PQQ10_03725) gshB 773086..774033 (-) 948 WP_000593252.1 glutathione synthase -
  PQQ10_RS03730 (PQQ10_03730) rsmE 774053..774784 (-) 732 WP_001675718.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PQQ10_RS03735 (PQQ10_03735) endA 774861..775568 (-) 708 WP_000286124.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36140.27 Da        Isoelectric Point: 7.0922

>NTDB_id=784046 PQQ10_RS03705 WP_274896048.1 770045..771025(+) (pilT) [Salmonella enterica subsp. enterica serovar Dublin strain RM100]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRLANELFPESDVDTLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGHLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QVQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=784046 PQQ10_RS03705 WP_274896048.1 770045..771025(+) (pilT) [Salmonella enterica subsp. enterica serovar Dublin strain RM100]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCTTGGCGAACGAGCTTTTTCCCGAGAGTGATGTCGACACGCTTCTGCTTGATTGGCTTAATG
ATGCGCAACAATATCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAAT
CCACTACGCTGGCGGCGATGGTGGGACATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTACGTGAAGATCCCGATGTCATTTTACTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGTCAACTGGCGGGTAGTCTGCGCGCTGTACTATCGCAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGTGCAGGGACGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(76-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.318

100

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.318

100

0.485
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baylyi ADP1

44.954

100

0.451
  pilT Acinetobacter baumannii D1279779

44.343

100

0.445
  pilT Acinetobacter baumannii strain A118

44.343

100

0.445
  pilT Pseudomonas stutzeri DSM 10701

45.886

96.933

0.445
  pilT Pseudomonas aeruginosa PAK

45.57

96.933

0.442
  pilT Acinetobacter nosocomialis M2

45.141

97.853

0.442
  pilT Legionella pneumophila strain ERS1305867

44.969

97.546

0.439
  pilT Legionella pneumophila strain Lp02

44.969

97.546

0.439
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

38.871

97.853

0.38
  pilU Pseudomonas stutzeri DSM 10701

36.446

100

0.371
  pilU Acinetobacter baylyi ADP1

36.533

99.08

0.362