Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PQP80_RS03735 Genome accession   NZ_CP117329
Coordinates   779665..780645 (+) Length   326 a.a.
NCBI ID   WP_274880268.1    Uniprot ID   -
Organism   Salmonella enterica subsp. enterica serovar Muenster strain RM074     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 774665..785645
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PQP80_RS03705 (PQP80_03705) - 775243..776250 (+) 1008 WP_023137235.1 DUF1202 domain-containing protein -
  PQP80_RS03710 (PQP80_03710) hemW 776341..777477 (-) 1137 WP_001096532.1 radical SAM family heme chaperone HemW -
  PQP80_RS03715 (PQP80_03715) - 777470..778063 (-) 594 WP_001174769.1 XTP/dITP diphosphatase -
  PQP80_RS03720 (PQP80_03720) yggU 778071..778361 (-) 291 WP_001277203.1 DUF167 family protein YggU -
  PQP80_RS03725 (PQP80_03725) - 778358..778924 (-) 567 WP_001094848.1 YggT family protein -
  PQP80_RS03730 (PQP80_03730) - 778943..779647 (-) 705 WP_023137234.1 YggS family pyridoxal phosphate-dependent enzyme -
  PQP80_RS03735 (PQP80_03735) pilT 779665..780645 (+) 981 WP_274880268.1 type IV pilus twitching motility protein PilT Machinery gene
  PQP80_RS03740 (PQP80_03740) - 780777..781463 (+) 687 WP_000098339.1 IclR family transcriptional regulator -
  PQP80_RS03745 (PQP80_03745) ruvX 781510..781926 (-) 417 WP_000017096.1 Holliday junction resolvase RuvX -
  PQP80_RS03750 (PQP80_03750) - 781926..782489 (-) 564 WP_001053167.1 YqgE/AlgH family protein -
  PQP80_RS03755 (PQP80_03755) gshB 782705..783652 (-) 948 WP_023137233.1 glutathione synthase -
  PQP80_RS03760 (PQP80_03760) rsmE 783672..784403 (-) 732 WP_001222488.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PQP80_RS03765 (PQP80_03765) endA 784480..785187 (-) 708 WP_000286123.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36239.47 Da        Isoelectric Point: 7.5116

>NTDB_id=783863 PQP80_RS03735 WP_274880268.1 779665..780645(+) (pilT) [Salmonella enterica subsp. enterica serovar Muenster strain RM074]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMENAPFTAPDVDRLLFDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFIHQQGTSLALRLLPERCPDLAEIQTPPIVPVLLASENGLILVTGATGCGKSTTLAAMVGYLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPIRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=783863 PQP80_RS03735 WP_274880268.1 779665..780645(+) (pilT) [Salmonella enterica subsp. enterica serovar Muenster strain RM074]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGAACGCGCCTTTTACCGCGCCTGATGTCGACAGGCTTCTGTTTGATTGGCTTAATG
ATGCGCAACAATATCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCATACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGTACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAGT
CCACCACGCTGGCGGCGATGGTGGGATATCTTAATCAACATGCCGATAAACATATCCTGACGCTGGAAGATCCTATTGAA
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTACGTGAAGATCCCGATGTCATTTTACTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACTGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGATACGCAGTCAACTGGCGGGTAGTCTGCGCGCCGTACTATCACAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGACTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(110-240)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.93

100

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.93

100

0.491
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter baumannii D1279779

45.566

100

0.457
  pilT Acinetobacter baumannii strain A118

45.566

100

0.457
  pilT Acinetobacter nosocomialis M2

45.26

100

0.454
  pilT Acinetobacter baylyi ADP1

45.26

100

0.454
  pilT Pseudomonas stutzeri DSM 10701

44.954

100

0.451
  pilT Pseudomonas aeruginosa PAK

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.654

97.546

0.436
  pilT Legionella pneumophila strain ERS1305867

44.654

97.546

0.436
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

38.871

97.853

0.38
  pilU Pseudomonas stutzeri DSM 10701

36.254

100

0.368
  pilU Acinetobacter baylyi ADP1

36.842

99.08

0.365