Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   PNF30_RS06680 Genome accession   NZ_CP116774
Coordinates   1315154..1317478 (+) Length   774 a.a.
NCBI ID   WP_205184367.1    Uniprot ID   -
Organism   Bacillus safensis strain SRCM125915     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1310154..1322478
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PNF30_RS06660 (PNF30_06660) - 1311381..1311677 (+) 297 WP_003216788.1 post-transcriptional regulator -
  PNF30_RS06665 (PNF30_06665) secDF 1311719..1313947 (+) 2229 WP_144471246.1 protein translocase subunit SecDF -
  PNF30_RS06670 (PNF30_06670) - 1314133..1314621 (+) 489 WP_041115109.1 cation:proton antiporter regulatory subunit -
  PNF30_RS06675 (PNF30_06675) - 1314722..1315072 (+) 351 WP_095409182.1 LapA family protein -
  PNF30_RS06680 (PNF30_06680) recJ 1315154..1317478 (+) 2325 WP_205184367.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  PNF30_RS06685 (PNF30_06685) - 1317502..1318014 (+) 513 WP_095409180.1 adenine phosphoribosyltransferase -
  PNF30_RS06690 (PNF30_06690) - 1318157..1320367 (+) 2211 WP_047203353.1 RelA/SpoT family protein -
  PNF30_RS06695 (PNF30_06695) dtd 1320386..1320829 (+) 444 WP_272510858.1 D-aminoacyl-tRNA deacylase -

Sequence

Protein


Download         Length: 774 a.a.        Molecular weight: 87092.67 Da        Isoelectric Point: 4.9514

>NTDB_id=780171 PNF30_RS06680 WP_205184367.1 1315154..1317478(+) (recJ) [Bacillus safensis strain SRCM125915]
MLLSKMRWEYEHPSEEKVKSLSENLNISALTASLLVKRGLEEVEEARSFLFDQKAEFHDPFLLKGMKEAVERINKAIEEQ
ESIVIFGDYDADGVTSTSVLLHTLKERSAIVDFYIPDRFKEGYGPNEQAFRYIKEQGASLVITVDTGIAAVNEARIAKEI
GLDLIITDHHEPSEELPEALAIVHPKQPGCEYPFKELAGVGVAFKVAHALLGKLPVQLLDLAAIGTIADLVPLHDENRWL
AKKGLVQLRQTNRPGLKALLKEAGATLEEANEETVGFQIAPRLNAVGRIEQADPAVHLLMTEDKDEAEELARFVQELNKE
RQKIVSTITEEAIQLVEEADDDQSAIVVAKAGWNPGVVGIVASKLTDTFSRPAIVLGIDEETQMAKGSARSIPGFDLFFH
LSKCRDILPHFGGHPMAAGMTLRADDVDLLRERLNQYANETLTEEDFIPIQRVDAVCKVDELTVEAIEEAGMLSPFGMSN
PKPFIMIEDAKLEDIRTIGANHNHIKMSLKDDDKLLDCVGFHQGKLKEEIVPGSRISVIGEMSVNEWNNRKKPQLMLKDA
RVDEWQLFDLRGKKDWEKKVSTLDSEKSLIVCFDEATKQDVDIPIHHVHAENVSSFNVHGKYVVFADVPADEQLLKQLLE
NQKPARIYTVFQRKEDHFMSSFPSRDQFKWFYGFLFKRGSFPIKEQGMELAKHRGWTKDTIIFMTKVFFELGFVKIENGV
LSIVLDAPKKDLTASASYTAKQRLMELDQTLTYSSAKELKEWLNSMMSEVSPVL

Nucleotide


Download         Length: 2325 bp        

>NTDB_id=780171 PNF30_RS06680 WP_205184367.1 1315154..1317478(+) (recJ) [Bacillus safensis strain SRCM125915]
ATGTTATTATCCAAAATGCGCTGGGAATATGAACATCCAAGCGAAGAAAAAGTGAAATCACTCTCTGAAAATCTGAATAT
TTCAGCGCTGACTGCATCGCTTCTCGTAAAAAGAGGACTTGAAGAAGTAGAAGAGGCGAGGTCTTTTTTATTTGATCAAA
AAGCCGAATTTCATGATCCATTCCTATTAAAAGGAATGAAAGAAGCAGTAGAGCGCATCAACAAAGCGATCGAAGAGCAG
GAATCGATTGTGATCTTCGGAGACTATGATGCAGATGGTGTGACAAGTACGTCTGTACTTTTACATACATTAAAAGAGCG
GTCTGCGATAGTAGACTTTTATATTCCAGATCGATTTAAAGAAGGCTATGGGCCTAATGAACAGGCATTTCGATATATCA
AGGAGCAAGGAGCATCGCTTGTCATCACGGTCGATACAGGCATTGCTGCTGTCAATGAAGCACGTATTGCAAAAGAAATA
GGGTTAGACCTAATCATTACAGATCACCATGAACCAAGTGAGGAGCTGCCTGAGGCACTTGCCATTGTCCATCCTAAGCA
GCCGGGCTGTGAGTATCCCTTTAAAGAACTTGCTGGTGTAGGTGTCGCGTTTAAAGTGGCGCATGCACTTCTTGGGAAGC
TGCCAGTTCAGCTGTTAGATCTTGCAGCAATCGGGACAATCGCAGATCTTGTACCTCTTCATGATGAGAATAGGTGGCTC
GCCAAAAAAGGTCTTGTGCAGCTTCGACAGACGAATAGACCGGGGCTGAAAGCGTTATTGAAAGAAGCGGGAGCCACTCT
AGAAGAAGCCAATGAGGAAACGGTCGGCTTTCAAATTGCGCCAAGGCTGAATGCTGTTGGGCGAATTGAACAGGCAGATC
CAGCCGTTCATCTTCTTATGACTGAGGATAAGGATGAAGCAGAAGAGCTCGCTCGTTTTGTACAGGAACTGAATAAAGAA
CGACAAAAGATTGTCAGTACGATCACAGAAGAAGCGATTCAATTGGTGGAAGAGGCAGATGATGACCAGTCTGCGATCGT
TGTAGCGAAAGCTGGCTGGAATCCGGGCGTTGTCGGAATTGTTGCCTCTAAATTGACGGATACTTTCTCTCGTCCGGCGA
TTGTACTTGGAATAGATGAAGAGACGCAAATGGCAAAAGGATCAGCCCGGAGTATTCCTGGCTTCGATCTATTTTTCCAT
CTAAGCAAATGCCGCGACATCCTGCCGCATTTCGGCGGGCATCCGATGGCAGCAGGGATGACACTTCGAGCGGATGATGT
CGATTTACTAAGGGAAAGATTAAATCAATATGCAAATGAAACATTGACGGAAGAAGATTTTATTCCAATCCAGCGAGTCG
ATGCAGTGTGTAAGGTAGACGAATTGACGGTTGAAGCGATTGAAGAAGCGGGGATGCTGTCGCCATTCGGTATGTCAAAC
CCAAAACCGTTCATTATGATTGAAGATGCGAAGCTTGAAGATATCCGAACGATTGGGGCAAATCACAACCATATTAAAAT
GTCTCTGAAAGACGATGACAAGCTGCTTGATTGTGTCGGTTTTCACCAAGGGAAATTAAAAGAAGAAATTGTTCCAGGAA
GCCGCATTTCCGTGATTGGAGAAATGTCCGTCAATGAGTGGAATAACCGGAAAAAACCCCAGCTCATGCTAAAAGATGCA
CGAGTTGATGAATGGCAGCTGTTTGATCTGCGTGGGAAAAAGGATTGGGAGAAGAAAGTTTCGACGCTTGATTCAGAAAA
ATCTTTGATTGTCTGTTTTGATGAAGCAACAAAACAGGACGTCGATATCCCTATTCATCATGTTCATGCAGAAAATGTAT
CATCGTTTAATGTACACGGAAAGTATGTGGTCTTTGCGGATGTTCCCGCAGATGAGCAATTGCTGAAGCAGCTTTTAGAA
AACCAAAAGCCTGCCAGAATATATACGGTTTTTCAAAGAAAAGAAGATCATTTTATGTCCTCTTTCCCAAGCCGTGACCA
ATTCAAATGGTTTTATGGGTTCTTGTTTAAACGGGGAAGTTTTCCTATCAAAGAACAAGGAATGGAGCTTGCCAAACATA
GAGGCTGGACAAAAGATACAATCATTTTTATGACAAAGGTGTTTTTTGAACTCGGTTTTGTTAAAATAGAGAATGGTGTG
CTGTCAATAGTACTTGATGCCCCTAAAAAAGATTTAACGGCATCAGCGTCCTATACAGCAAAACAACGATTGATGGAACT
CGATCAAACACTAACATATTCCTCAGCTAAAGAGCTGAAGGAATGGCTGAATAGCATGATGAGCGAAGTCTCACCTGTGC
TTTGA

Domains

Predicted by InterproScan.

DHHA1 domain (DHHA1)

(312-437)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-763)

RecJ OB domain (RecJ_OB)

(453-559)

DHH family (DHH)

(83-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

67.31

100

0.676