Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   POF58_RS01920 Genome accession   NZ_CP116711
Coordinates   332056..333345 (+) Length   429 a.a.
NCBI ID   WP_000432962.1    Uniprot ID   -
Organism   Streptococcus agalactiae strain CNCTC 10/84 Cas9 AEKOrevertant     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 327056..338345
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  POF58_RS01900 (POF58_01900) - 328363..329031 (+) 669 WP_000394891.1 fructose-6-phosphate aldolase -
  POF58_RS01905 (POF58_01905) - 329099..330187 (+) 1089 WP_000862118.1 glycerol dehydrogenase -
  POF58_RS01910 (POF58_01910) cysK 330339..331265 (-) 927 WP_000036948.1 cysteine synthase A -
  POF58_RS01915 (POF58_01915) - 331356..332000 (-) 645 WP_001108150.1 YigZ family protein -
  POF58_RS01920 (POF58_01920) comFA/cflA 332056..333345 (+) 1290 WP_000432962.1 DEAD/DEAH box helicase Machinery gene
  POF58_RS01925 (POF58_01925) - 333345..334010 (+) 666 WP_000128288.1 ComF family protein -
  POF58_RS01930 (POF58_01930) raiA 334087..334641 (+) 555 WP_000599096.1 ribosome-associated translation inhibitor RaiA -

Sequence

Protein


Download         Length: 429 a.a.        Molecular weight: 48671.48 Da        Isoelectric Point: 9.9941

>NTDB_id=779463 POF58_RS01920 WP_000432962.1 332056..333345(+) (comFA/cflA) [Streptococcus agalactiae strain CNCTC 10/84 Cas9 AEKOrevertant]
MENYLGRLWTKAQLSEQLRKIAISLPSFIKKGSDYICTRCSSSVAKNCQLPTGNYYCRECIVFGRVTSNENLYYFPQKTF
SKTNSLKWKGGLTPYQNEVSEELLKGISSKENLLVHAVTGAGKTEMIYHSVAKVIDTGGSVCIASPRIDVCLELHKRLSN
DFRCAITLMHGESPSYQRSPLTIATTHQLLKFYHAFDLLIVDEVDAFPYVDNPILYHGVKQALKENGTSIFLTATSTTEL
ERKVARKELKKLHLARRFHANPLVIPEMVWVSGIQKSLQTQKLPPKLYQLINKQRQTRYPLLLFFPHISEGQVFTEILRQ
AFPMEKIGFVSSKSTSRLKLVQDFRDNKLSILVSTTILERGVTFPSVDVFVIQANHHLFTKSSLVQISGRVGRALERPEG
LLYFLHDGKSKSMHQAIKEIKNMNHIGGF

Nucleotide


Download         Length: 1290 bp        

>NTDB_id=779463 POF58_RS01920 WP_000432962.1 332056..333345(+) (comFA/cflA) [Streptococcus agalactiae strain CNCTC 10/84 Cas9 AEKOrevertant]
ATGGAAAATTATTTAGGTAGGCTTTGGACAAAAGCACAATTATCTGAGCAACTTAGAAAAATTGCAATTAGCTTGCCATC
TTTTATAAAGAAAGGAAGCGACTATATTTGTACTCGATGCTCTAGTTCTGTTGCTAAAAATTGCCAATTACCAACTGGAA
ATTATTATTGTAGAGAATGTATAGTTTTTGGAAGAGTTACTAGTAATGAAAATTTATATTATTTTCCACAAAAAACATTT
TCTAAAACGAATTCTTTAAAATGGAAAGGGGGACTGACGCCTTATCAAAATGAGGTTTCAGAAGAGTTGTTGAAAGGTAT
TTCAAGTAAGGAAAATCTTCTTGTTCATGCTGTGACAGGGGCTGGTAAAACAGAAATGATATACCACTCTGTCGCCAAAG
TAATTGATACTGGAGGAAGCGTTTGTATTGCCAGTCCTAGAATTGATGTTTGTTTAGAATTGCATAAGCGACTTTCAAAC
GATTTTAGATGTGCCATAACATTAATGCATGGAGAATCTCCTTCTTATCAGAGATCCCCTTTAACTATTGCCACAACACA
TCAATTGTTAAAATTTTATCATGCTTTTGATTTGCTAATTGTTGATGAAGTTGATGCTTTTCCATATGTCGACAACCCGA
TACTATACCATGGTGTAAAACAGGCTTTAAAGGAAAACGGAACAAGTATTTTTCTGACTGCAACTTCAACGACTGAGTTA
GAAAGAAAAGTTGCAAGAAAAGAACTAAAAAAATTGCATCTAGCTAGGAGATTTCATGCCAATCCGTTAGTGATACCCGA
GATGGTTTGGGTAAGTGGTATACAGAAAAGCTTGCAGACACAAAAACTACCTCCCAAATTATATCAGTTGATAAATAAAC
AGCGACAAACGAGATATCCTTTGTTATTATTTTTCCCCCATATTTCAGAAGGTCAGGTATTCACCGAAATTTTAAGACAA
GCCTTTCCTATGGAAAAAATTGGTTTTGTTTCAAGTAAGAGCACATCTAGGTTAAAGTTAGTGCAAGATTTTCGAGATAA
CAAATTGTCTATCCTAGTGTCAACTACCATTCTAGAGAGAGGAGTAACTTTCCCTAGCGTCGACGTTTTTGTTATACAAG
CAAATCATCATTTGTTTACGAAAAGTTCCTTGGTGCAAATTTCAGGAAGAGTTGGAAGAGCATTAGAAAGGCCCGAAGGG
CTCCTTTACTTCCTTCATGATGGGAAAAGTAAATCGATGCACCAAGCTATAAAGGAGATTAAAAACATGAATCATATAGG
AGGATTTTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(286-394)

DEAD/DEAH box helicase (DEAD)

(93-240)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis NCTC 12261

60.89

99.534

0.606
  comFA/cflA Streptococcus pneumoniae Rx1

60.422

99.534

0.601
  comFA/cflA Streptococcus pneumoniae D39

60.422

99.534

0.601
  comFA/cflA Streptococcus pneumoniae R6

60.422

99.534

0.601
  comFA/cflA Streptococcus mitis SK321

60.422

99.534

0.601
  comFA/cflA Streptococcus pneumoniae TIGR4

60.422

99.534

0.601
  comFA Lactococcus lactis subsp. cremoris KW2

50

91.841

0.459
  comFA Bacillus subtilis subsp. subtilis str. 168

39.312

94.872

0.373
  comFA Latilactobacillus sakei subsp. sakei 23K

36.322

100

0.368