Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   POF62_RS05070 Genome accession   NZ_CP116707
Coordinates   945808..946665 (+) Length   285 a.a.
NCBI ID   WP_000163020.1    Uniprot ID   A0AAV3JMI0
Organism   Streptococcus agalactiae strain A909 sCas9revertant     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 940808..951665
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  POF62_RS05040 (POF62_05040) - 941124..942005 (+) 882 WP_000919085.1 F0F1 ATP synthase subunit gamma -
  POF62_RS05045 (POF62_05045) atpD 942079..943485 (+) 1407 WP_000094375.1 F0F1 ATP synthase subunit beta -
  POF62_RS05050 (POF62_05050) - 943498..943911 (+) 414 WP_000068054.1 F0F1 ATP synthase subunit epsilon -
  POF62_RS05055 (POF62_05055) - 943976..944206 (+) 231 WP_000602601.1 DUF1146 family protein -
  POF62_RS05060 (POF62_05060) murA 944269..945540 (+) 1272 WP_000357877.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  POF62_RS05065 (POF62_05065) - 945542..945733 (+) 192 WP_000168511.1 DNA-directed RNA polymerase subunit beta -
  POF62_RS05070 (POF62_05070) endA 945808..946665 (+) 858 WP_000163020.1 DNA/RNA non-specific endonuclease Machinery gene
  POF62_RS05075 (POF62_05075) pheS 946957..947997 (+) 1041 WP_000365655.1 phenylalanine--tRNA ligase subunit alpha -
  POF62_RS05080 (POF62_05080) - 948080..948601 (+) 522 WP_000078400.1 GNAT family N-acetyltransferase -
  POF62_RS05085 (POF62_05085) pheT 948655..951060 (+) 2406 WP_000961592.1 phenylalanine--tRNA ligase subunit beta -

Sequence

Protein


Download         Length: 285 a.a.        Molecular weight: 31352.34 Da        Isoelectric Point: 10.4835

>NTDB_id=779271 POF62_RS05070 WP_000163020.1 945808..946665(+) (endA) [Streptococcus agalactiae strain A909 sCas9revertant]
MTKKQKQITASIVTLLFSLLAIGLGESKSLPNDHILKQVTDLIISNESGKNTSLKNVSGTPSRELSESVLTSNVKKQLGT
NIAWNQSGAFIINQNKTDLNAKVSSAPYAINEIKKVNNQIVPTKANALLTKATRQYRNREETGNGRTYWKPAGWHQINGL
KGSYNHAVDRGHLIGYALVGSLRGFDASTSNPKNIATQAAWANQANSNQSTGQNYYETLVRKALDRHKTVRYRVTLIYDR
DNLLSSGSHIEAKSSDGSLEFNVFIPNVQSGLLFDYATGKVKQTK

Nucleotide


Download         Length: 858 bp        

>NTDB_id=779271 POF62_RS05070 WP_000163020.1 945808..946665(+) (endA) [Streptococcus agalactiae strain A909 sCas9revertant]
ATGACAAAAAAACAAAAACAAATAACAGCTTCGATTGTAACTTTGCTTTTTTCATTACTGGCAATAGGGTTAGGTGAAAG
CAAATCTTTGCCTAATGATCACATTTTAAAACAGGTGACAGATTTAATTATTAGTAATGAATCTGGCAAAAATACTTCCC
TTAAAAATGTATCAGGAACTCCTAGTAGAGAGTTATCTGAATCTGTTTTGACTAGCAATGTTAAAAAACAATTGGGAACA
AATATAGCTTGGAACCAGTCAGGTGCTTTTATCATTAATCAAAATAAAACAGATTTGAATGCTAAAGTGTCAAGTGCACC
ATATGCTATTAATGAAATAAAGAAGGTTAATAACCAAATTGTTCCGACTAAAGCAAATGCCTTATTAACAAAAGCAACTC
GTCAATATCGAAATCGAGAAGAAACAGGGAATGGTAGAACTTATTGGAAACCAGCTGGATGGCATCAGATAAATGGATTA
AAAGGGAGCTATAACCACGCTGTGGACAGAGGCCATTTAATTGGTTATGCTTTAGTAGGGAGTTTAAGAGGATTTGATGC
CTCCACAAGTAATCCTAAAAATATTGCCACACAAGCAGCTTGGGCAAACCAAGCTAATAGCAACCAATCAACAGGGCAAA
ATTATTATGAAACTTTAGTTCGTAAAGCATTGGATCGTCATAAAACTGTTAGGTATCGTGTGACTTTAATTTATGACAGA
GATAATCTTTTATCTTCGGGATCACATATTGAGGCTAAATCTTCAGATGGAAGTTTAGAATTTAACGTTTTTATTCCTAA
TGTACAATCGGGATTATTATTTGATTATGCAACAGGAAAAGTGAAGCAGACAAAGTAA

Domains

Predicted by InterproScan.

DNA/RNA non-specific endonuclease (Endonuclease_NS)

(87-243)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

66.379

81.404

0.54
  endA Streptococcus pneumoniae D39

66.379

81.404

0.54
  endA Streptococcus pneumoniae R6

66.379

81.404

0.54
  endA Streptococcus pneumoniae TIGR4

66.379

81.404

0.54