Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   PJW02_RS24800 Genome accession   NZ_CP116313
Coordinates   4785936..4789451 (-) Length   1171 a.a.
NCBI ID   WP_000058598.1    Uniprot ID   A0A9W5QJU2
Organism   Bacillus thuringiensis strain Lip     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4780936..4794451
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PJW02_RS24780 (PJW02_24780) - 4781132..4781518 (+) 387 WP_000902319.1 spore germination protein GerPE -
  PJW02_RS24785 (PJW02_24785) gerPF 4781561..4781776 (+) 216 WP_001141566.1 spore germination protein GerPF -
  PJW02_RS24790 (PJW02_24790) - 4781914..4782201 (-) 288 WP_000255727.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  PJW02_RS24795 (PJW02_24795) addA 4782214..4785939 (-) 3726 WP_000572259.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  PJW02_RS24800 (PJW02_24800) addB 4785936..4789451 (-) 3516 WP_000058598.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  PJW02_RS24805 (PJW02_24805) lepB 4789568..4790131 (-) 564 WP_000751910.1 signal peptidase I -
  PJW02_RS24810 (PJW02_24810) - 4790188..4790781 (-) 594 WP_000347518.1 TVP38/TMEM64 family protein -
  PJW02_RS24815 (PJW02_24815) - 4791117..4791698 (-) 582 WP_000444439.1 competence protein ComK -
  PJW02_RS24820 (PJW02_24820) - 4791983..4792168 (+) 186 WP_000391373.1 hypothetical protein -
  PJW02_RS24825 (PJW02_24825) - 4792517..4792720 (+) 204 WP_000356923.1 hypothetical protein -
  PJW02_RS24830 (PJW02_24830) cspA 4792918..4793121 (-) 204 WP_000301518.1 RNA chaperone/antiterminator CspA -
  PJW02_RS24835 (PJW02_24835) - 4793405..4793899 (+) 495 WP_000182395.1 DUF6584 family protein -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134140.38 Da        Isoelectric Point: 5.1777

>NTDB_id=776588 PJW02_RS24800 WP_000058598.1 4785936..4789451(-) (addB) [Bacillus thuringiensis strain Lip]
MSLRFVIGRAGSGKSTLCLREVQEELKQRPRGKTILYLVPEQMTFQTQQALIGSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDAHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLVEKLPQSEYVNGAEIYIDGFHSFSPQELEIVRQLMICGARVTITLTIDE
KTLAQPVNELDLFYETTLTYEKIKQVAREEKIEIEKTIPLMEQPRFHSPALAHLEAHYEARPNEKFNGEASVTISTAANL
RAEVEGVAREIRKLVAEENYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKETMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDTNGMITDSEREMEEKINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEELRIRAEESGDFLFATDHEQVWEEVMSLLDTFVEMLGEEK
MSLSMFTDVMSTGLEALQFANIPPSLDQVLIANIDHSRLSDVKATFIIGVNEGVIPAAPMDEGMLSDEEREVLGAAGIEL
APTTRQTLLEEQFVMYQMVTRASEKLYISCPLADEEGKTLLASSFIKKIKRMFPNVKDSFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAKKLSTAVSRDLYGDIIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKKIADKLLRENRTWADLSIKECEHLSVLVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSIILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFSLPNGVKMEVVGRIDRVDKAEDENG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTSNAQTWMKKGHAASPAGVLYFHIHNPIVEMKGDASEAEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHTFENIGKDITEGVIDIAP
YKMGNKAACTFCNFKSVCQFDESLEDNQFRSLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=776588 PJW02_RS24800 WP_000058598.1 4785936..4789451(-) (addB) [Bacillus thuringiensis strain Lip]
ATGTCACTTCGATTTGTGATTGGTAGAGCGGGAAGTGGAAAAAGTACACTTTGTTTACGCGAAGTGCAAGAAGAGTTAAA
ACAACGCCCGAGAGGGAAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAGACGCAGCAGGCGTTAATTGGAA
GTGAGGATGTAAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCACGATTAGCGTGGAAGGTACTACAAGAAGTTGGC
GGAGCGAGTCGTCTTCATATTGATGAAGCGGGTGTACATATGTTACTCCGTAAAATTGTAGAGTCTCGTAAAGATGGATT
ATCGGTGTTCCAAAAAGCAGCGGAGCAAAACGGTTTCTTTGAACATCTTGGCAGTATGATTGCAGAGTTTAAACGTTACA
ATGTAACGCCATCTAACGTATATGAAATGTGGCAACAATTGGATGCGCATAGTAGCAGTGCCGAACAAAAGTTACTAGCG
AATAAAGTGTATGATTTACAGCTATTATACGATGATTTTGAACGTGCTTTAATTGGAAAATATTTAGATTCAGAAGACTA
CCTACAATTGCTAGTGGAAAAGCTTCCGCAGTCTGAATATGTAAATGGTGCGGAAATTTATATAGATGGATTTCATTCAT
TTTCCCCGCAAGAGTTAGAGATTGTAAGACAGCTTATGATTTGCGGAGCGAGAGTTACGATCACGTTAACGATAGATGAA
AAAACGTTAGCACAGCCAGTAAATGAACTCGATTTATTTTATGAAACGACGTTAACGTATGAAAAAATCAAACAAGTAGC
GCGTGAAGAGAAAATAGAAATTGAAAAAACAATTCCACTTATGGAACAGCCGCGTTTTCATTCTCCGGCATTAGCGCATT
TAGAAGCTCATTATGAAGCGCGTCCGAATGAAAAATTTAACGGTGAAGCGAGTGTAACGATTAGTACAGCTGCGAATTTA
CGAGCAGAGGTAGAAGGTGTTGCGCGTGAAATTCGTAAACTTGTGGCGGAGGAAAACTATCGTTACCGAGATATTGCGGT
TCTTCTTCGTAATGGGGAAAGTTATTACGATGTGATGCGAACGTTATTTACAGATTATAATATCCCACACTTCATCGATG
AAAAGCGCCCGATGTCACACCACCCATTAGTAGAATGTATTCGTTCTGCACTCGAGATTATTAGTGGGAATTGGCGTTAT
GATGCGGTGTTCCGCTGCGTGAAAACAGAGCTTTTATATCCATTAGACGTAAGAAAAGAAACGATGCGTGAAGAAATGGA
TGAATTTGAAAATTACTGTTTAGCGTACGGTGTACAAGGAAAGAGGTGGACTTCAGAGGATCCATGGATGTATCGCCGTT
ATCGTTCTCTTGATGATACGAACGGGATGATCACAGATAGTGAACGTGAAATGGAAGAGAAAATAAATCGATTGCGTGAC
GTTGTAAGAACACCTGTTATTCGCATGCAAAAAAGGTTAAAGCGTGCAGGAACAGTTATGCAAATGTGTGAAGCAGTTTA
CTTATTTCTAGAAGAGCTGGATGTTCCGAAAAAACTAGAAGAATTACGTATACGTGCAGAAGAAAGTGGAGATTTCTTAT
TTGCGACAGATCATGAACAAGTATGGGAAGAAGTAATGAGCCTTCTTGATACGTTTGTAGAGATGCTTGGTGAAGAGAAA
ATGTCTCTTTCTATGTTTACGGACGTTATGTCGACAGGTCTTGAGGCACTTCAATTTGCTAACATTCCGCCGTCATTAGA
CCAAGTGTTAATTGCTAATATTGATCACTCCAGGTTATCTGACGTGAAAGCAACGTTTATTATAGGTGTAAATGAGGGTG
TTATTCCAGCAGCACCTATGGACGAAGGTATGCTTTCTGATGAAGAAAGAGAAGTTCTTGGTGCAGCAGGGATTGAATTA
GCGCCAACGACGAGACAAACTTTGTTAGAAGAACAGTTCGTTATGTATCAAATGGTAACGAGAGCATCTGAGAAGTTATA
TATTTCATGCCCGCTTGCAGATGAAGAAGGAAAGACGTTACTTGCGTCTAGCTTTATTAAGAAAATAAAAAGAATGTTCC
CTAATGTGAAAGATTCATTTATTACCAATGACGTAAACGATTTATCACGTTCAGAACAAATTTCATACGTAGCAACGCCA
GAAGTAACGTTATCTTACGTTATGCAGCAACTACAAACGTGGAAGCGATACGGATTTGAAGGGAATTTAGACTTTTGGTG
GGACGTATATAATTTCTACGTAACTTCAGATGAATGGAAACAAAAAAGTAGCCGCGTCTTATCAAGTTTATTCTATCGAA
ATCGTGCGAAGAAACTAAGTACAGCAGTAAGTAGAGATTTATACGGAGATATAATTAAAGGAAGCGTCTCTCGTATGGAA
CTATTTAACCGTTGTGCATACGCTCATTTTGCGCAGCACGGTTTATCGCTTAGAGAGCGTGATATTTTCAAGCTTGATGC
GCCGGACATCGGCGAATTATTCCATGCGGCATTAAAGAAAATTGCGGACAAGCTATTGCGTGAAAACCGTACTTGGGCGG
ATTTATCCATAAAAGAGTGTGAGCATCTTTCTGTTTTAGTAATAGAAGAAATCGCACCGTTATTACAACGACAAATTTTA
TTAAGTTCAAACCGTCATTTCTATTTAAAACAAAAACTACAACAAATCATTTTCCGTACGTCAATCATTCTTCGTGAACA
TGCGAAGTCAAGCGGATTCGTACCAGTTGATTTAGAAGTGCCGTTCGGTATGGGTGGTACAGGATCACTTCCGCCGATGG
AATTCTCATTACCAAATGGTGTGAAGATGGAAGTTGTCGGCCGTATTGACCGTGTTGATAAGGCAGAAGATGAAAACGGG
ACGTTCTTACGTATTATTGATTATAAATCAAGTTCAAAAGCGTTAGATTTAACGGAAGTGTATTACGGATTAGCACTTCA
AATGTTAACGTATTTAGATGTTGTTACTTCGAATGCGCAGACGTGGATGAAAAAAGGTCACGCAGCATCACCAGCTGGTG
TATTGTATTTCCATATTCATAACCCAATCGTTGAGATGAAAGGTGACGCATCTGAAGCTGAAATTGAAAAAGAAATTTTA
AAGAAATTTAAAATGAAAGGACTCGTATTAGGAGATGCTGACGTTGTTCGTTTAATGGATAACAAACTTTCAACAGGAAG
TTCAGATATTATTTCTGCTGGCCTGAAAAAAGACGGTAGCTTTAGTGCGCGTTCAAGTATTGCAAGTGAGCAAGAGTTTA
ACGTACTGCAAAAATATGTACACCATACGTTTGAAAATATCGGAAAAGATATTACAGAAGGTGTTATCGATATTGCTCCT
TATAAAATGGGAAATAAAGCAGCATGTACGTTCTGTAACTTCAAATCTGTTTGTCAATTTGATGAGTCACTTGAAGATAA
TCAGTTCCGATCGTTAAAAGATATGAAAGATAGCGAAGCGATGGAGAAAATAAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(303-669)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.488

100

0.495