Detailed information NTG0776314

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PI990_RS02120
• Genome accession: NZ_CP116239
• Coordinates: 440471..441616 (+)
• Length: 381 a.a.
• NCBI ID: WP_021218362.1
• Uniprot ID: A0AAU7Y6D2
• Organism: Pseudomonas sp. TUM22785
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 435471..446616

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PI990_RS02095 (PI990_02095)	-	435735..436328 (-)	594	WP_184488410.1	YggT family protein	-
PI990_RS02100 (PI990_02100)	-	436491..437705 (-)	1215	WP_271659698.1	hypothetical protein	-
PI990_RS02105 (PI990_02105)	proC	437711..438529 (-)	819	WP_021218359.1	pyrroline-5-carboxylate reductase	-
PI990_RS02110 (PI990_02110)	-	438543..439238 (-)	696	WP_271659699.1	YggS family pyridoxal phosphate-dependent enzyme	-
PI990_RS02115 (PI990_02115)	pilT	439307..440341 (+)	1035	WP_021218361.1	type IV pilus twitching motility protein PilT	Machinery gene
PI990_RS02120 (PI990_02120)	pilU	440471..441616 (+)	1146	WP_021218362.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PI990_RS02125 (PI990_02125)	-	441549..443042 (-)	1494	WP_271659700.1	UdgX family uracil-DNA binding protein	-
PI990_RS02130 (PI990_02130)	-	443042..444256 (-)	1215	WP_263401653.1	putative DNA modification/repair radical SAM protein	-
PI990_RS02135 (PI990_02135)	-	444358..444751 (+)	394	Protein_426	TM2 domain-containing protein	-
PI990_RS02140 (PI990_02140)	-	444811..446082 (-)	1272	WP_265170288.1	dihydroorotase	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42463.75 Da
• Isoelectric Point: 6.5931

>NTDB_id=776314 PI990_RS02120 WP_021218362.1 440471..441616(+) (pilU) [Pseudomonas sp. TUM22785]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVTKNAMSPEQTRETVLAVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLDDLKLPEILKKLALTKRGLVLFVGATGTGKSTSLAAMIGYRNKNSSGHIISIED
PIEYIHQHQSCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEVRTRETMDHAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQQQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYTLYSQGEITYEDALLYADSANDLRLMIKLGSETDGDHLTSMSGGLSLEVSEEDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=776314 PI990_RS02120 WP_021218362.1 440471..441616(+) (pilU) [Pseudomonas sp. TUM22785]
ATGGAATTCGAGAAACTGTTGCGCCTGATGGTCGAGAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTCCCCCCGTC
GATGAAGGTCAACGGCAAGATCATGCCCGTGACCAAGAACGCCATGTCCCCGGAGCAGACCCGTGAAACCGTTCTCGCGG
TGATGAACGAGCAGCAGCGCCGTGACTTCGCCGAGAACCACGAGTGCAACTTCGCCATCAGCGCCCGGGGCATCGGCCGT
TTCCGCGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTGCTGCGCCGCATCGAGACCAACATCCCCACCCTGGA
TGACCTCAAGCTGCCGGAAATCCTCAAGAAGCTGGCACTGACCAAGCGCGGCCTGGTGCTCTTCGTCGGCGCCACCGGTA
CCGGCAAGTCCACTTCCCTGGCGGCGATGATCGGCTACCGCAACAAGAACAGCAGCGGCCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGAGCTGCATCGTCACCCAGCGTGAAGTGGGCATCGACACCGAGTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTCATCGGTGAGGTGCGGACCCGCGAAACCATGGACCATG
CCGTGGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATCAACTTCTTCCCTGCCGACCGTCAGCAGCAGGTGTGGATGGACCTGTCCCTCAACCTCAAGGCCATCGTCGCTCAGCA
ACTGGTGCCGACCCCGGACGGCAAGGGTCGCCGCGCGGTGATCGAGGTGCTGATCAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTCCACGAGCTCAAGCCGCTGATGAAGCGCTCCACCGAACAGGGCATGCAGACCTTCGACCAGGCG
CTGTACACCCTCTACTCCCAGGGCGAGATCACCTACGAAGACGCGCTGCTCTACGCTGACTCGGCGAACGACCTGCGCCT
GATGATCAAGCTCGGTTCCGAAACCGACGGCGACCACCTCACGAGCATGAGCGGTGGCCTGTCGCTGGAAGTGTCCGAGG
AAGACCCGGGCCGTCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (110-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	91.339	100	0.913
pilU	Acinetobacter baylyi ADP1	64.426	93.701	0.604
pilU	Vibrio cholerae strain A1552	58.857	91.864	0.541
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.155	88.189	0.381