Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   PF996_RS14515 Genome accession   NZ_CP116014
Coordinates   2822788..2825148 (-) Length   786 a.a.
NCBI ID   WP_257988787.1    Uniprot ID   -
Organism   Bacillus velezensis strain SRCM124633     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2817788..2830148
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PF996_RS14495 (PF996_14495) - 2817863..2819425 (+) 1563 WP_151140330.1 SH3 domain-containing protein -
  PF996_RS14500 (PF996_14500) dtd 2819452..2819895 (-) 444 WP_007408194.1 D-aminoacyl-tRNA deacylase -
  PF996_RS14505 (PF996_14505) relA 2819908..2822112 (-) 2205 WP_003152716.1 GTP diphosphokinase -
  PF996_RS14510 (PF996_14510) - 2822270..2822782 (-) 513 WP_003152714.1 adenine phosphoribosyltransferase -
  PF996_RS14515 (PF996_14515) recJ 2822788..2825148 (-) 2361 WP_257988787.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  PF996_RS14520 (PF996_14520) - 2825204..2825530 (-) 327 WP_003152709.1 LapA family protein -
  PF996_RS14525 (PF996_14525) - 2825594..2826091 (-) 498 WP_017418232.1 cation:proton antiporter regulatory subunit -
  PF996_RS14530 (PF996_14530) secDF 2826222..2828441 (-) 2220 WP_070082161.1 protein translocase subunit SecDF -
  PF996_RS14535 (PF996_14535) - 2828478..2828774 (-) 297 WP_007408189.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 786 a.a.        Molecular weight: 87252.68 Da        Isoelectric Point: 4.9874

>NTDB_id=775279 PF996_RS14515 WP_257988787.1 2822788..2825148(-) (recJ) [Bacillus velezensis strain SRCM124633]
MLASKMRWEIQQPDQEKVKSLTEQLDITPLVASLLVKRGFDTAESARLFLHTKDADFYDPYELKGMREAVDRIKLAVSQG
EPIMIYGDYDADGVTSTSVMLTALKQLSADVDFYIPDRFTEGYGPNEQAFRSIKERGFSLIITVDTGIAAVHEAEVAKEL
GLDVIITDHHEPGPELPDVFAIIHPKQPGCGYPFKELAGVGVAFKLAHALTGSLPEAMLDLAAIGTVADLVPLHDENRLI
ASLGIERLRTTNRPGLKALIKLSGGDIGQADEETIGFQVAPRLNAVGRIEQADPAVHLLMTEDQFEAEELASDIDRLNKE
RQKMVKTMTEEAVEMVQQQGGNPSAIVVAKAGWNPGVVGIVASKLAERFYRPAVVLGIDVEQGIAKGSARSIRGFNLFTS
LSQCRDILPHFGGHPMAAGMTLKAEDVAELRDRLNQMAEETLTEEDFIPVQEVDLVCGVEDITVESITELNMLSPFGMLN
PKPHVMVKNAVLDDVRLIGANKNHVKMTVKSDSAVLDCVGFNKGELEAGVVPGSTISIVGEMSINEWNNRKKPQLMIKDA
AVPEWQLFDIRGKRTWEDTVTALPKEKRVLISFNEKTAQKLREKELSGELAGITSESEAEALQLDGKYAVFLDVPPSADR
LQHLLDGKKPERIYFVFINHEEHFLSSFPSRNDFKWFYAFLLKRGAFDFGKHGSELARHRGWSIDTINFMTKVFFDLGFV
KIEDGVLSVVSGAKKRDLADSQTYQVKQQLMELDQKLNYSAAHELKEWLNKLMNQGSEAYESTRRT

Nucleotide


Download         Length: 2361 bp        

>NTDB_id=775279 PF996_RS14515 WP_257988787.1 2822788..2825148(-) (recJ) [Bacillus velezensis strain SRCM124633]
ATGTTAGCGTCAAAAATGCGCTGGGAAATCCAGCAGCCGGATCAGGAAAAAGTCAAATCACTCACAGAACAATTGGACAT
AACACCTCTTGTGGCATCACTGCTTGTAAAAAGAGGTTTTGATACTGCCGAAAGCGCAAGGCTGTTTTTACATACAAAGG
ATGCAGACTTTTATGACCCGTATGAATTAAAGGGAATGCGGGAAGCAGTTGATCGGATAAAACTGGCGGTTTCTCAAGGG
GAACCGATTATGATTTACGGAGATTACGATGCAGACGGCGTTACAAGCACGTCAGTGATGCTGACGGCACTCAAACAGCT
GTCGGCGGACGTTGACTTTTATATTCCCGACCGTTTTACAGAAGGCTACGGCCCGAATGAACAAGCTTTCCGCTCCATAA
AAGAGCGGGGATTTTCGCTGATCATTACAGTGGATACGGGAATCGCCGCCGTCCATGAAGCTGAAGTCGCAAAAGAGCTG
GGCTTAGACGTCATTATTACCGACCACCATGAGCCGGGACCCGAGCTTCCTGACGTATTTGCGATCATCCACCCGAAGCA
GCCGGGATGCGGCTATCCGTTTAAAGAATTGGCCGGCGTCGGTGTGGCGTTTAAGCTTGCCCACGCGCTTACGGGTTCTT
TGCCGGAAGCTATGCTGGACTTGGCGGCAATCGGTACGGTTGCAGATCTGGTGCCTCTTCATGATGAAAACAGATTAATT
GCGTCACTCGGAATTGAAAGGCTTCGGACGACAAATCGGCCGGGCCTCAAAGCTCTGATTAAGCTTTCCGGCGGAGATAT
CGGACAAGCTGATGAGGAAACGATCGGATTTCAGGTCGCACCGAGGCTCAATGCCGTCGGAAGAATAGAACAGGCCGATC
CCGCCGTTCACCTGCTGATGACCGAAGATCAGTTTGAAGCGGAGGAACTGGCTTCGGATATTGACCGGCTGAATAAAGAG
CGGCAGAAAATGGTGAAAACCATGACCGAAGAGGCTGTTGAAATGGTTCAGCAGCAAGGCGGAAACCCGTCTGCCATTGT
AGTGGCAAAAGCCGGCTGGAATCCGGGAGTCGTCGGTATTGTCGCATCGAAGCTGGCTGAACGTTTTTACCGGCCCGCTG
TTGTGCTTGGAATAGATGTGGAGCAGGGAATCGCTAAAGGGTCGGCGCGAAGCATCAGGGGCTTCAACCTTTTTACGAGC
CTTTCACAGTGCAGAGACATTCTTCCTCATTTCGGCGGGCATCCGATGGCGGCCGGAATGACGTTGAAGGCTGAAGATGT
GGCGGAATTAAGGGACAGGCTGAATCAAATGGCTGAAGAAACCCTGACGGAAGAGGATTTTATTCCCGTTCAGGAGGTTG
ATCTTGTCTGCGGCGTTGAAGACATAACCGTTGAAAGCATTACAGAATTGAATATGCTGTCGCCGTTCGGCATGCTTAAC
CCTAAACCGCATGTGATGGTGAAAAATGCCGTGCTTGATGATGTGCGCCTGATCGGAGCGAATAAAAATCACGTGAAAAT
GACGGTGAAAAGTGATTCCGCCGTCCTTGACTGCGTCGGCTTTAACAAAGGGGAGCTTGAAGCCGGAGTTGTCCCCGGAT
CAACGATTTCCATTGTCGGCGAGATGTCCATTAATGAATGGAACAACAGAAAGAAACCCCAGTTGATGATTAAAGATGCT
GCGGTGCCGGAATGGCAGCTTTTTGATATCAGGGGCAAGCGGACGTGGGAAGACACGGTCACCGCGCTGCCGAAGGAAAA
ACGGGTGCTCATCAGCTTTAACGAAAAAACCGCTCAGAAGCTTCGTGAAAAAGAGCTGTCGGGCGAGCTGGCCGGGATCA
CTTCGGAATCCGAGGCGGAAGCTCTCCAGCTTGACGGAAAATATGCCGTTTTTCTGGATGTGCCGCCGTCAGCGGATCGC
CTGCAGCATTTGCTTGACGGAAAGAAGCCGGAACGGATTTATTTTGTTTTTATCAATCATGAAGAACATTTCCTGTCCTC
ATTTCCATCGCGGAATGATTTCAAGTGGTTTTACGCATTTCTCTTAAAAAGGGGCGCTTTTGACTTCGGCAAGCATGGTT
CAGAGCTTGCGAGACACAGAGGATGGTCAATTGACACAATCAATTTCATGACAAAGGTGTTTTTTGATCTCGGTTTTGTT
AAAATAGAAGATGGTGTGCTGTCTGTCGTGAGCGGCGCGAAAAAACGCGACCTGGCAGATTCGCAAACCTATCAGGTAAA
ACAGCAGCTGATGGAATTAGACCAGAAGCTCAATTATTCCGCAGCCCATGAGCTGAAGGAATGGCTGAATAAGCTGATGA
ACCAAGGTTCAGAAGCTTATGAAAGTACGAGGAGGACATAG

Domains

Predicted by InterproScan.

DHHA1 domain (DHHA1)

(315-437)

RecJ OB domain (RecJ_OB)

(453-559)

DHH family (DHH)

(83-226)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-769)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

81.934

100

0.819