Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   PF977_RS07070 Genome accession   NZ_CP116012
Coordinates   1384132..1386492 (+) Length   786 a.a.
NCBI ID   WP_015714440.1    Uniprot ID   A0A8E0VQW3
Organism   Bacillus subtilis strain SRCM124333     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1379132..1391492
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PF977_RS07050 (PF977_07050) comN 1380463..1380759 (+) 297 WP_003229735.1 post-transcriptional regulator ComN -
  PF977_RS07055 (PF977_07055) secDF 1380798..1383011 (+) 2214 WP_015714442.1 protein translocase subunit SecDF -
  PF977_RS07060 (PF977_07060) yrvC 1383169..1383666 (+) 498 WP_015714441.1 cation:proton antiporter regulatory subunit -
  PF977_RS07065 (PF977_07065) yrvD 1383742..1384065 (+) 324 WP_003229740.1 LapA family protein -
  PF977_RS07070 (PF977_07070) recJ 1384132..1386492 (+) 2361 WP_015714440.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  PF977_RS07075 (PF977_07075) aptA 1386498..1387010 (+) 513 WP_003229745.1 adenine phosphoribosyltransferase -
  PF977_RS07080 (PF977_07080) relA 1387178..1389382 (+) 2205 WP_003229747.1 GTP diphosphokinase -
  PF977_RS07085 (PF977_07085) dtd 1389395..1389835 (+) 441 WP_004398689.1 D-aminoacyl-tRNA deacylase -
  PF977_RS07090 (PF977_07090) yrvJ 1389862..1391418 (-) 1557 WP_015714439.1 SH3 domain-containing protein -

Sequence

Protein


Download         Length: 786 a.a.        Molecular weight: 88019.66 Da        Isoelectric Point: 5.1797

>NTDB_id=775096 PF977_RS07070 WP_015714440.1 1384132..1386492(+) (recJ) [Bacillus subtilis strain SRCM124333]
MLASKMRWEIQRPDQDKVKSLTEQLHITPLVASLLVKRGFDTAESARLFLHTKDADFYDPFEMKGMKEAADRIKQAISQQ
EKIMIYGDYDADGVTSTSVMLHTLQKLSAQVDFYIPDRFKEGYGPNEQAFRSIKDRGFSLIITVDTGIAAVHEAKVAKEL
GLDVIITDHHEPGPELPDVHAIVHPKQPGCTYPFKELAGVGVAFKLAHALLGELPDELLDLAAIGTIADLVPLHDENRLI
ATLGLERLRRTNRLGLKELIKLSGGDIGEANEETVGFQLAPRLNAVGRIEQADPAVHLLMSEDSFEAEELAAEIDQLNKE
RQKMVSKMTDEAIEMVEQQGLDQTAIVVAKAGWNPGVVGIVASKLVDRFYRPAIVLGIDEEKGIAKGSARSIRGFNLFES
LSECRDILPHFGGHPMAAGMTLKAEDVPDLRSRLNEIADNTLTEEDFIPVQEVDLVCSVEDITVESIAEMNMLSPFGMLN
PKPHVLVENAVLEDVRKIGANKTHVKMTIRNESSQLDCVGFNKGELQEGIVPGSRISIVGEMSINEWNNRKKPQLMIKDA
AVSEWQLFDLRGKRTWEDTVSALPSAKRAIVSFKEDSTTLLQTEDLRREVHVISSKDQAKAFDLDGAYIVLLDPPPSLDM
LARLLEGKAPERIYFIFLNHEDHFLSTFPARDHFKWYYAFLLKRGAFDVKKHGSELAKHKGWSVETINFMTKVFFDLGFV
KIENGVLSVVSGAKKRDLTDSQTYQAKQQLMELDQKLNYSSAEELKEWLNKLMKQDSEAYESTRRT

Nucleotide


Download         Length: 2361 bp        

>NTDB_id=775096 PF977_RS07070 WP_015714440.1 1384132..1386492(+) (recJ) [Bacillus subtilis strain SRCM124333]
ATGTTAGCGTCAAAAATGCGATGGGAAATCCAGCGACCAGATCAGGATAAGGTCAAATCACTCACAGAACAATTGCATAT
AACACCTCTTGTTGCGTCCCTGCTTGTAAAAAGAGGCTTTGATACGGCAGAAAGCGCTAGGCTGTTTTTACATACAAAGG
ATGCCGATTTTTATGATCCTTTTGAAATGAAAGGCATGAAAGAAGCGGCAGATCGAATAAAACAGGCGATTTCACAACAA
GAAAAGATTATGATATATGGCGATTATGACGCCGATGGGGTTACAAGTACGTCAGTGATGCTTCACACGCTGCAAAAACT
GTCGGCTCAAGTTGATTTTTATATACCTGACCGTTTTAAAGAAGGATATGGACCTAATGAACAGGCGTTTCGTTCGATTA
AAGATCGGGGCTTCTCTTTGATCATTACGGTTGATACAGGAATTGCCGCTGTGCATGAAGCGAAGGTGGCAAAGGAACTC
GGACTGGATGTCATCATTACCGATCATCATGAACCGGGACCGGAGCTTCCGGATGTCCATGCGATCGTCCACCCGAAGCA
GCCTGGCTGCACTTACCCGTTTAAAGAGCTTGCCGGGGTTGGAGTCGCGTTTAAGCTTGCCCACGCTTTATTGGGTGAAC
TTCCTGATGAACTGCTTGACCTGGCGGCTATCGGCACCATTGCCGATCTAGTCCCGCTGCATGACGAAAACAGATTAATT
GCAACACTAGGACTTGAAAGGCTTCGACGGACAAATCGGCTCGGCTTAAAAGAGCTGATTAAGCTGTCAGGCGGAGACAT
CGGAGAAGCGAACGAAGAAACAGTCGGATTTCAGCTCGCACCAAGGCTGAATGCCGTCGGAAGAATCGAACAGGCGGATC
CCGCTGTTCATTTGCTTATGTCAGAGGATTCGTTTGAAGCGGAAGAGCTTGCCGCAGAAATTGACCAGCTGAACAAAGAG
CGCCAAAAAATGGTCAGCAAAATGACGGATGAAGCGATTGAAATGGTTGAACAGCAAGGGCTCGATCAAACTGCAATTGT
CGTGGCCAAAGCTGGATGGAACCCGGGAGTCGTCGGGATTGTGGCTTCAAAGCTTGTGGACCGCTTCTACCGACCGGCCA
TTGTTCTTGGCATTGATGAAGAAAAGGGAATCGCTAAAGGTTCGGCCCGAAGCATCAGAGGCTTTAATTTATTTGAGAGC
CTTTCTGAATGCAGAGATATTCTTCCTCATTTCGGCGGCCATCCAATGGCTGCGGGGATGACGCTGAAAGCTGAAGATGT
GCCGGATTTGCGAAGCAGGCTGAACGAAATAGCGGACAATACGCTTACGGAAGAGGATTTTATTCCCGTACAGGAAGTCG
ACCTTGTCTGCAGCGTCGAGGACATTACAGTTGAAAGCATCGCAGAAATGAACATGCTGTCTCCTTTTGGCATGCTTAAC
CCGAAACCGCACGTTCTCGTAGAAAACGCAGTACTGGAAGATGTCCGCAAGATAGGGGCAAATAAAACTCATGTCAAAAT
GACGATCAGAAACGAGTCGTCCCAGCTTGATTGCGTCGGTTTTAATAAAGGCGAGCTTCAAGAAGGCATCGTACCGGGTT
CAAGAATTTCCATTGTCGGTGAGATGTCAATCAACGAATGGAACAATCGAAAAAAGCCGCAGCTTATGATAAAGGATGCC
GCTGTTTCTGAATGGCAGCTGTTTGATTTAAGGGGAAAACGGACGTGGGAAGACACAGTTTCCGCCCTTCCATCTGCTAA
ACGGGCCATTGTCTCTTTCAAAGAAGACTCAACAACATTACTTCAAACTGAAGACCTTCGCCGAGAAGTGCACGTCATCA
GTTCTAAAGATCAAGCAAAGGCGTTTGACCTCGATGGTGCATATATTGTATTACTGGATCCGCCGCCTTCGCTGGACATG
CTCGCCCGTTTGCTGGAAGGCAAGGCGCCTGAGCGGATATATTTTATTTTTCTCAATCATGAGGACCACTTTTTATCAAC
TTTTCCCGCAAGAGACCACTTTAAATGGTATTATGCGTTCTTGCTGAAAAGAGGCGCTTTTGACGTGAAAAAACACGGTT
CAGAGCTTGCAAAACATAAAGGCTGGTCCGTTGAAACAATCAATTTCATGACAAAGGTGTTTTTTGATCTCGGTTTTGTT
AAAATAGAGAATGGTGTGCTGTCTGTTGTGAGCGGAGCAAAGAAACGCGACTTAACAGACTCACAAACATATCAGGCGAA
ACAGCAGCTGATGGAACTGGATCAAAAGCTGAATTATTCTTCTGCTGAAGAGCTGAAGGAATGGCTGAATAAGCTGATGA
AACAAGATTCAGAGGCTTATGAAAGTACGAGGAGGACATAA

Domains

Predicted by InterproScan.

RecJ OB domain (RecJ_OB)

(453-559)

DHH family (DHH)

(82-226)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-769)

DHHA1 domain (DHHA1)

(315-437)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

99.618

100

0.996