Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   OR232_RS01145 Genome accession   NZ_CP128596
Coordinates   251985..252749 (-) Length   254 a.a.
NCBI ID   WP_001136232.1    Uniprot ID   Q0TBX9
Organism   Escherichia coli strain muskdeer13-4     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 246985..257749
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OR232_RS01115 (OR232_001115) - 247619..248977 (-) 1359 WP_001306345.1 PTS galactitol transporter subunit IIC -
  OR232_RS01120 (OR232_001120) - 249054..249335 (-) 282 WP_000084021.1 PTS sugar transporter subunit IIB -
  OR232_RS01125 (OR232_001125) - 249332..249805 (-) 474 WP_001161647.1 PTS sugar transporter subunit IIA -
  OR232_RS01130 (OR232_001130) - 249830..250576 (-) 747 WP_001296489.1 GntR family transcriptional regulator -
  OR232_RS01135 (OR232_001135) nikR 250775..251176 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  OR232_RS01140 (OR232_001140) nikE 251182..251988 (-) 807 WP_000173679.1 nickel import ATP-binding protein NikE -
  OR232_RS01145 (OR232_001145) amiE 251985..252749 (-) 765 WP_001136232.1 nickel import ATP-binding protein NikD Regulator
  OR232_RS01150 (OR232_001150) nikC 252749..253582 (-) 834 WP_001008954.1 nickel ABC transporter permease subunit NikC -
  OR232_RS01155 (OR232_001155) nikB 253579..254523 (-) 945 WP_000947070.1 nickel ABC transporter permease subunit NikB -
  OR232_RS01160 (OR232_001160) nikA 254523..256097 (-) 1575 WP_000493122.1 nickel ABC transporter substrate-binding protein -
  OR232_RS01165 (OR232_001165) acpT 256208..256795 (-) 588 WP_000285789.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26812.40 Da        Isoelectric Point: 6.6882

>NTDB_id=774906 OR232_RS01145 WP_001136232.1 251985..252749(-) (amiE) [Escherichia coli strain muskdeer13-4]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGKIVEQGDVETLFNAPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=774906 OR232_RS01145 WP_001136232.1 251985..252749(-) (amiE) [Escherichia coli strain muskdeer13-4]
ATGCCACAACAGATTGAACTCCGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTATCGTTAACCCTGCA
ACGTGGGCGTGTGCTGGCGTTAGTCGGCGGTAGTGGCAGCGGGAAGTCGCTGACCTGCGCCGCGACGCTGGGCATTCTGC
CTGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTGGCCGATGGAAAACCGGTTTCTCCCTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGTGCCTTTAATCCGCTGCACACCATGCACACCCACGCGCGTGAAACCTGCCT
GGCGCTGGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTCTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGCGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGATCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCACCGGGAATGCTGCTGGTTACCCATGATATGGGCGTGGTAGCGCGTCTGGCAGACGATGTGGCGGTAA
TGTCTCACGGTAAGATTGTTGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATGCGGTAACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q0TBX9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398