Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   QUS04_RS02995 Genome accession   NZ_CP128592
Coordinates   421997..422464 (-) Length   155 a.a.
NCBI ID   WP_000339836.1    Uniprot ID   -
Organism   Escherichia coli strain VG12A2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 416997..427464
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUS04_RS02970 (QUS04_02970) rpsG 417138..417608 (+) 471 WP_001138043.1 30S ribosomal protein S7 -
  QUS04_RS02975 (QUS04_02975) fusA 417705..419819 (+) 2115 WP_000124700.1 elongation factor G -
  QUS04_RS02980 (QUS04_02980) tuf 419890..421074 (+) 1185 WP_000031783.1 elongation factor Tu -
  QUS04_RS02985 (QUS04_02985) bfd 421257..421451 (+) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  QUS04_RS02990 (QUS04_02990) bfr 421524..422000 (+) 477 WP_000675515.1 bacterioferritin -
  QUS04_RS02995 (QUS04_02995) pilD 421997..422464 (-) 468 WP_000339836.1 A24 family peptidase Machinery gene
  QUS04_RS03000 (QUS04_03000) rpsJ 422843..423154 (+) 312 WP_001181004.1 30S ribosomal protein S10 -
  QUS04_RS03005 (QUS04_03005) rplC 423187..423816 (+) 630 WP_000579833.1 50S ribosomal protein L3 -
  QUS04_RS03010 (QUS04_03010) rplD 423827..424432 (+) 606 WP_000424395.1 50S ribosomal protein L4 -
  QUS04_RS03015 (QUS04_03015) rplW 424429..424731 (+) 303 WP_000617544.1 50S ribosomal protein L23 -
  QUS04_RS03020 (QUS04_03020) rplB 424749..425570 (+) 822 WP_000301864.1 50S ribosomal protein L2 -
  QUS04_RS03025 (QUS04_03025) rpsS 425587..425865 (+) 279 WP_001138117.1 30S ribosomal protein S19 -
  QUS04_RS03030 (QUS04_03030) rplV 425880..426212 (+) 333 WP_000447529.1 50S ribosomal protein L22 -
  QUS04_RS03035 (QUS04_03035) rpsC 426230..426931 (+) 702 WP_000529945.1 30S ribosomal protein S3 -
  QUS04_RS03040 (QUS04_03040) rplP 426944..427354 (+) 411 WP_000941212.1 50S ribosomal protein L16 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16951.08 Da        Isoelectric Point: 8.1296

>NTDB_id=774749 QUS04_RS02995 WP_000339836.1 421997..422464(-) (pilD) [Escherichia coli strain VG12A2]
MDANLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFSQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=774749 QUS04_RS02995 WP_000339836.1 421997..422464(-) (pilD) [Escherichia coli strain VG12A2]
ATGGACGCTAACCTTCCCTTTCTGATTTTGTACGCTTGTTTATCTGTCCTACTTTTTTTATGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTCTCAGGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACCTGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTGTTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGTTGGGATAGTTTGCTAGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(10-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.8

80.645

0.361