Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   QUS04_RS02290 Genome accession   NZ_CP128592
Coordinates   275523..276287 (-) Length   254 a.a.
NCBI ID   WP_001136229.1    Uniprot ID   B7L5T2
Organism   Escherichia coli strain VG12A2     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 270523..281287
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUS04_RS02265 (QUS04_02265) yhhJ 272379..273503 (+) 1125 WP_001314210.1 ABC transporter permease -
  QUS04_RS02270 (QUS04_02270) - 273607..273837 (+) 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin -
  QUS04_RS02275 (QUS04_02275) - 273834..274193 (+) 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin -
  QUS04_RS02280 (QUS04_02280) nikR 274313..274714 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  QUS04_RS02285 (QUS04_02285) nikE 274720..275526 (-) 807 WP_000173667.1 nickel import ATP-binding protein NikE -
  QUS04_RS02290 (QUS04_02290) amiE 275523..276287 (-) 765 WP_001136229.1 nickel import ATP-binding protein NikD Regulator
  QUS04_RS02295 (QUS04_02295) nikC 276287..277120 (-) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  QUS04_RS02300 (QUS04_02300) nikB 277117..278061 (-) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  QUS04_RS02305 (QUS04_02305) nikA 278061..279635 (-) 1575 WP_000953356.1 nickel ABC transporter substrate-binding protein -
  QUS04_RS02310 (QUS04_02310) acpT 279746..280333 (-) 588 WP_000285784.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26820.38 Da        Isoelectric Point: 6.3761

>NTDB_id=774744 QUS04_RS02290 WP_001136229.1 275523..276287(-) (amiE) [Escherichia coli strain VG12A2]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=774744 QUS04_RS02290 WP_001136229.1 275523..276287(-) (amiE) [Escherichia coli strain VG12A2]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCTGACGGTAAGATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACAGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7L5T2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398