Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   QR290_RS25225 Genome accession   NZ_CP128260
Coordinates   5618058..5618459 (-) Length   133 a.a.
NCBI ID   WP_085648738.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain PH.SM     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5613058..5623459
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QR290_RS25205 (QR290_25205) - 5613698..5613931 (+) 234 WP_115079251.1 PP0621 family protein -
  QR290_RS25210 (QR290_25210) pilS 5613921..5615510 (+) 1590 WP_289205331.1 ATP-binding protein Regulator
  QR290_RS25215 (QR290_25215) - 5615507..5616853 (+) 1347 WP_115079253.1 sigma-54 dependent transcriptional regulator -
  QR290_RS25220 (QR290_25220) thiO 5616900..5618000 (-) 1101 WP_289203831.1 glycine oxidase ThiO -
  QR290_RS25225 (QR290_25225) comF 5618058..5618459 (-) 402 WP_085648738.1 type IV pilin protein Machinery gene
  QR290_RS25230 (QR290_25230) - 5618475..5621567 (-) 3093 WP_289203832.1 PilC/PilY family type IV pilus protein -
  QR290_RS25235 (QR290_25235) - 5621554..5622033 (-) 480 WP_115079256.1 PilX N-terminal domain-containing pilus assembly protein -
  QR290_RS25240 (QR290_25240) - 5622044..5622757 (-) 714 WP_115079257.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  QR290_RS25245 (QR290_25245) pilV 5622754..5623203 (-) 450 WP_011336000.1 type IV pilus modification protein PilV -

Sequence

Protein


Download         Length: 133 a.a.        Molecular weight: 14633.77 Da        Isoelectric Point: 9.7660

>NTDB_id=773470 QR290_RS25225 WP_085648738.1 5618058..5618459(-) (comF) [Pseudomonas fluorescens strain PH.SM]
MRRSNRGFTLIEIMIVIAIIGIVITIGYPSLTEYVKKGRRADVVSTLSEQAQILERFYSKNNVYTGVTGLSTGNDFYTIT
PTITDQTFLLTATRKTGTAMATDKCGDFTLTNTGVRSMNNATTGLTTKDCWGR

Nucleotide


Download         Length: 402 bp        

>NTDB_id=773470 QR290_RS25225 WP_085648738.1 5618058..5618459(-) (comF) [Pseudomonas fluorescens strain PH.SM]
ATGCGCAGATCCAACCGAGGCTTCACCCTGATCGAAATCATGATCGTGATTGCGATCATCGGTATCGTCATCACCATCGG
CTATCCGAGCCTCACCGAATACGTGAAGAAGGGCCGCCGCGCCGACGTGGTCAGCACTTTGTCGGAACAGGCACAGATCC
TCGAACGCTTCTATTCGAAGAACAACGTCTACACCGGTGTCACCGGGTTGAGCACCGGCAACGATTTCTACACCATCACG
CCGACGATCACCGATCAGACCTTCCTGCTGACCGCGACCCGCAAGACCGGCACGGCGATGGCCACTGACAAGTGCGGGGA
TTTCACCCTCACCAACACCGGTGTCAGAAGCATGAACAACGCGACCACCGGGCTGACCACCAAGGATTGCTGGGGCCGCT
GA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-28)

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-109)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

35.211

100

0.376