Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   O3276_RS13230 Genome accession   NZ_CP114771
Coordinates   3239793..3240905 (+) Length   370 a.a.
NCBI ID   WP_269671773.1    Uniprot ID   -
Organism   Endozoicomonas sp. GU-1 strain Ap1-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3234793..3245905
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O3276_RS13200 (O3276_13200) - 3234900..3235268 (+) 369 WP_269671768.1 glutaredoxin -
  O3276_RS13205 (O3276_13205) - 3235276..3237071 (+) 1796 Protein_2591 DEAD/DEAH box helicase -
  O3276_RS13210 (O3276_13210) - 3237071..3237583 (+) 513 WP_269671769.1 hypothetical protein -
  O3276_RS13215 (O3276_13215) - 3237643..3237993 (+) 351 WP_269671770.1 hypothetical protein -
  O3276_RS13220 (O3276_13220) - 3237939..3238682 (-) 744 WP_269671771.1 type 1 glutamine amidotransferase -
  O3276_RS13225 (O3276_13225) - 3239261..3239746 (+) 486 WP_269671772.1 glutathione peroxidase -
  O3276_RS13230 (O3276_13230) pilU 3239793..3240905 (+) 1113 WP_269671773.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  O3276_RS13235 (O3276_13235) - 3240958..3241446 (-) 489 WP_269671774.1 GNAT family N-acetyltransferase -
  O3276_RS13240 (O3276_13240) - 3241626..3242063 (+) 438 WP_269671775.1 hypothetical protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41157.81 Da        Isoelectric Point: 6.0655

>NTDB_id=767007 O3276_RS13230 WP_269671773.1 3239793..3240905(+) (pilU) [Endozoicomonas sp. GU-1 strain Ap1-2]
MDINKALSLLADRNGSELFLTVGYPPCLKINDQLVPIANTSLTSEQAHHSFATMMGEERFKEFCATRESNYAIQNPESGR
FRISAFFQRGEPGMVIRRIHHHIPSPRELGLPDIFCDLMLQERGLILITGPAGAGKTTAMASLVNHRNTAGKGHIITIED
PVEFIHSHRQCIVTQREVGLDTQSYEEGLSNALRQAPDLVVIGEIRTPEVMKHTIEFVQTGHLCIATLHANTAYQALERI
IHFFPREQHEEVLLDLSLSLQGILGQQLLSSEDGTAAYPSHEILLNTPALADLIKKGKVDEIHDLMERSKDSGMQTIDQS
IFKLFEAGKITAQQAIQHADSSNNMRLLIKMHGHQEGDDNSLPHLTISTD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=767007 O3276_RS13230 WP_269671773.1 3239793..3240905(+) (pilU) [Endozoicomonas sp. GU-1 strain Ap1-2]
GTGGATATTAACAAAGCACTCTCCCTGCTGGCTGATCGTAACGGTTCAGAACTGTTTCTGACGGTCGGCTACCCCCCCTG
TTTGAAAATCAATGATCAATTGGTACCGATAGCCAACACCTCGCTGACCAGCGAGCAGGCCCACCATAGTTTTGCAACCA
TGATGGGCGAAGAGCGCTTTAAAGAATTCTGTGCGACCCGTGAAAGCAATTACGCGATACAGAACCCGGAGTCCGGTCGT
TTTCGCATCAGTGCGTTCTTCCAAAGAGGGGAACCCGGAATGGTGATCCGGCGCATTCACCACCATATTCCTTCCCCCCG
GGAGCTTGGCCTGCCGGATATTTTCTGCGACCTGATGCTTCAGGAAAGAGGTTTGATCCTGATTACCGGGCCAGCAGGTG
CCGGTAAAACAACCGCGATGGCATCCCTGGTGAACCATCGCAACACTGCGGGTAAAGGGCATATCATCACCATTGAAGAC
CCTGTTGAATTTATCCACAGCCACAGACAATGCATCGTCACCCAGCGAGAAGTTGGACTGGACACCCAATCTTATGAAGA
AGGTCTGTCGAACGCCCTGCGTCAGGCACCCGACCTGGTGGTGATTGGTGAGATCCGCACCCCCGAGGTGATGAAACATA
CCATTGAATTTGTGCAAACGGGTCACCTCTGTATTGCAACCCTGCATGCCAACACAGCCTATCAGGCGCTGGAACGCATC
ATTCACTTCTTCCCCCGGGAGCAACATGAAGAGGTTCTTCTGGATTTATCACTGAGCTTGCAGGGAATCCTGGGGCAACA
GCTGCTCAGCTCTGAAGATGGTACGGCCGCCTATCCTTCCCATGAAATCCTGTTAAATACACCGGCGCTGGCCGATCTCA
TCAAAAAGGGCAAAGTGGATGAAATCCATGACTTGATGGAGCGCTCCAAAGACTCGGGTATGCAAACCATTGACCAATCC
ATATTCAAGCTGTTTGAGGCTGGAAAAATCACCGCTCAACAAGCTATCCAGCATGCTGACTCCTCCAATAACATGCGCCT
GCTCATAAAGATGCATGGTCATCAGGAAGGGGACGATAATTCCCTGCCACACTTGACCATTTCCACGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-243)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

48.907

98.919

0.484
  pilU Acinetobacter baylyi ADP1

49.716

95.135

0.473
  pilU Vibrio cholerae strain A1552

47.911

97.027

0.465