Detailed information NTG0766937

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: O4M85_RS02280
• Genome accession: NZ_CP114761
• Coordinates: 502607..503755 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain NF143349
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 497607..508755

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
O4M85_RS02255 (O4M85_02255)	-	498758..499054 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
O4M85_RS02260 (O4M85_02260)	-	499051..499644 (-)	594	WP_003084544.1	YggT family protein	-
O4M85_RS02265 (O4M85_02265)	proC	499655..500476 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
O4M85_RS02270 (O4M85_02270)	-	500488..501180 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
O4M85_RS02275 (O4M85_02275)	pilT	501395..502429 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
O4M85_RS02280 (O4M85_02280)	pilU	502607..503755 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
O4M85_RS02285 (O4M85_02285)	czcD	503763..504662 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
O4M85_RS02290 (O4M85_02290)	-	504816..505220 (+)	405	WP_003084560.1	NINE protein	-
O4M85_RS02295 (O4M85_02295)	-	505463..506836 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
O4M85_RS02300 (O4M85_02300)	-	506833..508017 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=766937 O4M85_RS02280 WP_003100959.1 502607..503755(+) (pilU) [Pseudomonas aeruginosa strain NF143349]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=766937 O4M85_RS02280 WP_003100959.1 502607..503755(+) (pilU) [Pseudomonas aeruginosa strain NF143349]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382