Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   O4M77_RS12875 Genome accession   NZ_CP114606
Coordinates   2705056..2705523 (+) Length   155 a.a.
NCBI ID   WP_286421227.1    Uniprot ID   -
Organism   Acinetobacter sp. YWS30-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 2701171..2716872 2705056..2705523 within 0


Gene organization within MGE regions


Location: 2701171..2716872
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O4M77_RS12850 (O4M77_12840) - 2701171..2702103 (-) 933 WP_180025590.1 IS5 family transposase -
  O4M77_RS12855 (O4M77_12845) - 2702183..2703379 (-) 1197 WP_323713535.1 serine hydrolase domain-containing protein -
  O4M77_RS12860 (O4M77_12850) - 2703463..2703873 (-) 411 WP_323713536.1 pilin -
  O4M77_RS12865 (O4M77_12855) - 2704284..2704439 (+) 156 WP_323714109.1 DUF1778 domain-containing protein -
  O4M77_RS12870 (O4M77_12860) - 2704509..2704703 (+) 195 WP_323714110.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
  O4M77_RS12875 (O4M77_12865) pilA 2705056..2705523 (+) 468 WP_286421227.1 pilin Machinery gene
  O4M77_RS12880 (O4M77_12870) - 2705610..2706515 (+) 906 WP_323713537.1 hypothetical protein -
  O4M77_RS12885 (O4M77_12875) - 2706730..2707086 (-) 357 WP_323713538.1 hypothetical protein -
  O4M77_RS12890 (O4M77_12880) - 2707090..2709147 (-) 2058 WP_323713539.1 hypothetical protein -
  O4M77_RS12895 (O4M77_12885) - 2709144..2711081 (-) 1938 WP_323713541.1 hypothetical protein -
  O4M77_RS12900 (O4M77_12890) - 2711078..2712505 (-) 1428 WP_323713542.1 site-specific integrase -
  O4M77_RS12905 (O4M77_12895) - 2712935..2713879 (+) 945 WP_323713543.1 Arc family DNA-binding protein -
  O4M77_RS12910 (O4M77_12900) - 2713940..2714146 (-) 207 WP_004281170.1 helix-turn-helix domain-containing protein -
  O4M77_RS12915 (O4M77_12905) - 2714306..2714572 (+) 267 WP_178319230.1 hypothetical protein -
  O4M77_RS12920 (O4M77_12910) - 2714678..2715298 (+) 621 WP_242757031.1 HAD family hydrolase -
  O4M77_RS12925 (O4M77_12915) - 2715341..2716117 (-) 777 WP_227560706.1 hypothetical protein -
  O4M77_RS12930 (O4M77_12920) - 2716342..2716872 (-) 531 WP_242757033.1 hypothetical protein -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 15673.88 Da        Isoelectric Point: 6.2119

>NTDB_id=766844 O4M77_RS12875 WP_286421227.1 2705056..2705523(+) (pilA) [Acinetobacter sp. YWS30-1]
MNAQKGFTLIELMIVVAIIGILAAIAIPAYQDYTVRAKVTEGLNLASPAKAIVAENASNAQPFARGYDGAKTANVDSITI
AEGTGAITITYTAAAGATTGADTIVLTPTYKATSTATAQTALSGTATASTVPTGAIEWACTGTMPNKYLPANCRA

Nucleotide


Download         Length: 468 bp        

>NTDB_id=766844 O4M77_RS12875 WP_286421227.1 2705056..2705523(+) (pilA) [Acinetobacter sp. YWS30-1]
ATGAACGCTCAAAAAGGCTTTACGCTCATCGAGTTGATGATTGTTGTCGCTATTATCGGTATTCTTGCTGCAATTGCGAT
TCCTGCTTATCAAGACTACACGGTGCGTGCTAAAGTAACAGAAGGTTTAAATTTAGCTAGTCCAGCAAAAGCAATCGTTG
CTGAAAATGCATCTAATGCACAACCTTTTGCACGTGGTTATGATGGTGCTAAAACTGCTAATGTAGACAGCATTACAATT
GCAGAAGGTACAGGTGCTATTACTATTACTTATACTGCAGCAGCAGGTGCTACAACAGGCGCTGATACTATTGTTTTAAC
CCCAACATATAAAGCAACTTCAACTGCAACTGCTCAAACAGCTTTGTCTGGTACAGCAACAGCAAGTACAGTACCTACAG
GCGCAATTGAGTGGGCATGTACTGGAACTATGCCTAATAAATATTTACCAGCGAACTGTCGTGCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-154)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

52.907

100

0.587
  pilA2 Legionella pneumophila str. Paris

54.43

100

0.555
  pilA2 Legionella pneumophila strain ERS1305867

53.797

100

0.548
  comP Acinetobacter baylyi ADP1

47.403

99.355

0.471
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

36.041

100

0.458
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40

100

0.413
  pilA Acinetobacter baumannii strain A118

39.49

100

0.4
  pilA Pseudomonas aeruginosa PAK

34.884

100

0.387
  pilA/pilA1 Eikenella corrodens VA1

47.619

81.29

0.387
  pilA/pilAII Pseudomonas stutzeri DSM 10701

36.842

98.065

0.361