Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   O0R52_RS05730 Genome accession   NZ_CP114066
Coordinates   1169966..1173466 (+) Length   1166 a.a.
NCBI ID   WP_217827178.1    Uniprot ID   -
Organism   Bacillus halotolerans strain B13     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1164966..1178466
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O0R52_RS05710 (O0R52_05710) - 1165820..1167021 (-) 1202 Protein_1063 MDR family MFS transporter -
  O0R52_RS05715 (O0R52_05715) - 1167137..1168867 (+) 1731 WP_217827179.1 SgrR family transcriptional regulator -
  O0R52_RS05720 (O0R52_05720) - 1168901..1169215 (-) 315 WP_044156484.1 four-helix bundle copper-binding protein -
  O0R52_RS05725 (O0R52_05725) - 1169344..1169781 (-) 438 WP_044156482.1 ferritin family protein -
  O0R52_RS05730 (O0R52_05730) addB 1169966..1173466 (+) 3501 WP_217827178.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  O0R52_RS05735 (O0R52_05735) addA 1173453..1177157 (+) 3705 WP_217827177.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  O0R52_RS05740 (O0R52_05740) sbcD 1177229..1178407 (+) 1179 WP_106294530.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 134725.76 Da        Isoelectric Point: 5.4792

>NTDB_id=764366 O0R52_RS05730 WP_217827178.1 1169966..1173466(+) (addB) [Bacillus halotolerans strain B13]
MGAEFLVGRSGSGKTKLIIDSIQDELRRAPFGKPIIFLVPDQMTFLMEYELAKTPDTGGMIRAQVFSFSRLAWRVLQHTG
GMSRPFLTSTGVQMLLRKLIEEHKQEFKVYQKASDKSGFTAQVERMLTEFKRYCLEPEDIRRMADSGTASEYRGERVLSE
KLHDLSILYQQMEKSLEDQYLHSEDYLTLLAEHIPQAEDLKGAHVYVDGFYQFTPQEFRVLEQLMLQAEHITFSLTADKP
SHEGEPQELDLFRMTGKTYYRLHQAAKELNLDITYKELDESKRHKRAPELAHLEAQYEARPVIPFAEKQEAFTVMQAANR
RAELEGIAREIHSLVREKGYRYKDMAILARQPKDYKDMVKEVFADYEIPYFIDGKTSMLNHPLVEFIRSSLDVLKGNWRY
EAVFRCVKTELLFPLNQPKAKVREQVDQLENYCIAYGIKGDRWTKGDRFHYRRFVSLDDEFAQTDQEIEMENMLNDTRDW
IVPPLFQLQKRMKKAKTVQEKAEALYRYLEETDVPLKLDQERQRAEEDGRIIEAQQHQQAWDAVIQLLEEFVEMMGEDDI
SLDLFQQMMETGAESLTFSLIPPALDQVFVGNMDLSRIYGTSCTFVIGANDGVLPARPDENGVLSDDDREWLKTIGIELS
SGGRERLLDEHFLIYMALSSPSDRLYVTYPIADAEGKTLLPSIVIKRLEELFPQHRERLLTNEPEQVSDEEQLMYLVNKS
VAQSYTASQLRLWTREYEISDVWWSAYNALMNEPDRLESKKLFSSLFFRNEVKQLERSVSRQLYGERIQGSVSRMETFNA
CPFSHFASHGLQLKERQFFKLEAPDIGQLFHSSLKLISDRLREHKIDWRDLTKEQCELFSYDAVERLAPKLQKEILLSSN
RHYYVKEKLQKIVTRVSGILSEHAKASGFVPIGLELGFGGKGPLPPLTFKLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSLDWLGMKATPAGVLYFHIHDPMIQSNLPLGLDEIEQEIFKKFKMKGL
LLGDQEAIRLMDTTLQEGRSNIINAGLKKDGSLRSDSAAVGEKEFELLTKHVRRTFQEAGEQITDGRVSIEPYKMKNKTP
CTYCAFKSVCQFDESLEENEYRLLKAEKDKTVLDWIKKEADGNEHS

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=764366 O0R52_RS05730 WP_217827178.1 1169966..1173466(+) (addB) [Bacillus halotolerans strain B13]
TTGGGAGCAGAGTTTTTAGTAGGCAGGTCCGGGAGTGGAAAAACGAAGCTGATCATCGACAGTATTCAAGATGAACTGCG
CCGGGCTCCATTCGGGAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATACGAGCTTGCTAAAA
CGCCTGATACGGGCGGCATGATTCGTGCTCAAGTCTTCAGCTTTTCACGACTGGCCTGGCGCGTCCTCCAGCATACGGGA
GGAATGAGCAGGCCGTTTCTTACGAGCACGGGCGTACAGATGCTCCTGCGGAAGCTCATTGAAGAGCACAAACAGGAGTT
TAAAGTCTATCAAAAAGCAAGTGATAAAAGCGGTTTTACCGCACAGGTAGAACGGATGCTGACTGAGTTTAAGAGATACT
GTCTGGAACCGGAAGATATTCGCCGGATGGCGGATAGCGGAACAGCTTCCGAGTATCGGGGGGAACGTGTTTTATCTGAA
AAGCTTCATGATTTATCGATCCTGTACCAGCAGATGGAAAAAAGCCTTGAGGATCAGTATCTTCATTCTGAGGATTATTT
GACACTATTGGCAGAGCATATCCCGCAGGCGGAAGATTTGAAAGGAGCACATGTCTATGTAGACGGCTTTTATCAGTTTA
CACCGCAGGAATTCAGGGTGTTAGAGCAGCTTATGCTTCAGGCAGAGCATATCACGTTTTCACTCACAGCGGACAAGCCG
TCACATGAGGGGGAGCCGCAGGAACTTGATTTGTTCAGAATGACGGGAAAAACGTATTATCGCTTGCACCAGGCTGCAAA
GGAACTGAATCTGGACATCACCTATAAAGAGCTTGATGAATCAAAGCGGCACAAACGCGCACCGGAATTGGCCCATTTAG
AAGCACAGTATGAAGCGCGTCCGGTTATTCCGTTTGCTGAGAAACAAGAGGCTTTTACTGTGATGCAGGCCGCTAACAGA
CGAGCAGAGCTGGAAGGTATTGCCCGGGAAATCCACAGTCTTGTCAGAGAGAAGGGCTACCGTTATAAGGATATGGCGAT
TCTAGCGCGTCAGCCGAAAGACTATAAGGATATGGTCAAGGAAGTATTTGCAGATTACGAGATTCCTTATTTTATTGACG
GGAAAACTTCTATGCTGAACCACCCGTTAGTAGAATTTATCCGGTCAAGCCTTGACGTATTAAAGGGAAATTGGCGTTAT
GAAGCGGTGTTCCGCTGCGTGAAAACCGAACTGTTATTCCCGCTTAACCAGCCGAAGGCCAAGGTAAGAGAGCAGGTTGA
TCAGCTGGAGAATTACTGTATCGCTTACGGGATCAAAGGCGATCGCTGGACAAAGGGCGATCGTTTCCATTACAGACGTT
TTGTGTCGTTAGATGATGAATTTGCGCAGACCGATCAAGAAATCGAAATGGAAAACATGCTGAATGACACGCGCGATTGG
ATTGTTCCGCCGCTGTTCCAGCTGCAGAAACGAATGAAAAAAGCAAAAACGGTTCAAGAGAAGGCGGAGGCGCTTTACCG
TTATTTAGAAGAGACGGATGTGCCGCTGAAGCTGGATCAGGAAAGACAGCGGGCTGAGGAAGACGGCAGAATCATTGAAG
CACAGCAGCACCAGCAGGCGTGGGATGCAGTCATTCAGCTGCTTGAGGAGTTTGTGGAAATGATGGGCGAAGATGACATT
TCTCTCGATTTGTTTCAGCAAATGATGGAGACCGGTGCAGAGTCGCTGACATTCTCTCTCATCCCTCCGGCGCTTGATCA
GGTGTTTGTCGGCAATATGGATTTGTCCAGAATATACGGCACCTCATGCACCTTTGTGATCGGGGCAAACGACGGCGTCC
TGCCGGCTCGCCCTGACGAAAACGGGGTGCTGTCGGATGATGATCGTGAGTGGCTGAAGACGATAGGTATTGAGCTTTCC
TCCGGCGGGCGGGAGCGTCTGCTTGATGAGCATTTCCTCATCTATATGGCGCTTTCAAGCCCTTCTGACCGCCTTTATGT
GACGTATCCGATTGCTGATGCGGAAGGGAAAACGCTTTTGCCGTCGATTGTAATCAAGCGGCTGGAAGAACTGTTTCCGC
AGCATCGTGAGCGTCTATTGACAAATGAACCGGAGCAGGTCAGTGATGAGGAACAGCTTATGTACCTTGTCAATAAAAGC
GTGGCACAATCATATACCGCTAGCCAGCTCAGATTATGGACGAGGGAATATGAGATCAGTGATGTCTGGTGGAGCGCGTA
CAATGCGCTGATGAATGAACCTGACAGGCTGGAGTCGAAAAAGCTTTTCTCTAGCCTGTTTTTCCGAAATGAAGTCAAGC
AGCTTGAACGCTCTGTATCGAGACAGCTCTATGGGGAACGCATTCAAGGGAGCGTGTCGAGGATGGAAACCTTTAACGCT
TGTCCGTTCTCCCATTTTGCGTCCCACGGGCTGCAGCTGAAGGAACGTCAATTTTTTAAGCTTGAAGCACCGGATATCGG
GCAGCTCTTTCATTCGAGTTTAAAACTGATTTCAGACCGGCTGCGCGAGCACAAAATAGATTGGCGCGACCTAACGAAGG
AGCAGTGCGAGCTGTTTTCTTATGATGCGGTCGAGCGGCTGGCGCCGAAACTGCAAAAAGAAATTCTGCTCAGTTCAAAC
AGACATTATTACGTGAAGGAAAAACTGCAAAAAATTGTGACACGCGTGTCCGGCATTTTAAGCGAGCATGCGAAAGCGAG
CGGATTCGTGCCGATCGGCCTTGAATTGGGCTTTGGCGGAAAGGGCCCGCTCCCGCCGCTTACCTTTAAGCTGAAAAACG
GCTGTACGATGGAGCTCGTCGGGCGGATTGACCGCGTGGATAAAGCGGAAAGCTCTAAAGGCCTGCTCCTCCGAATTGTC
GACTATAAATCCAGCGATAAAGGCCTTGACTTGGCGGAAGTGTATTACGGACTTGCGCTGCAAATGCTGACGTATCTGGA
TCTATCGATTACACATTCGTTAGACTGGCTAGGAATGAAAGCCACGCCGGCCGGAGTGCTGTATTTCCATATTCATGACC
CGATGATACAATCGAATCTTCCGCTCGGACTTGATGAAATTGAACAGGAAATTTTTAAGAAATTTAAGATGAAGGGCCTC
CTCCTCGGTGATCAGGAAGCGATTCGCCTCATGGATACAACCCTTCAAGAAGGAAGATCAAATATCATCAATGCGGGCCT
GAAGAAAGACGGCTCACTCAGATCGGATTCAGCGGCAGTCGGTGAAAAAGAATTTGAGCTGTTGACGAAGCATGTGCGCC
GCACCTTCCAAGAAGCAGGCGAGCAAATCACTGACGGGCGCGTATCCATTGAGCCGTATAAAATGAAGAACAAGACGCCT
TGCACATACTGTGCGTTCAAATCAGTATGCCAATTTGATGAATCATTAGAAGAAAACGAGTATCGTCTGTTAAAGGCCGA
AAAGGACAAGACGGTACTTGATTGGATAAAGAAGGAGGCTGATGGCAATGAACATTCCTAA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(306-668)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

94.254

100

0.943