Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   OTU47_RS04255 Genome accession   NZ_CP113115
Coordinates   791645..792469 (+) Length   274 a.a.
NCBI ID   WP_001036784.1    Uniprot ID   A0A0E7E4I0
Organism   Streptococcus pneumoniae strain NP2     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 786645..797469
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OTU47_RS04220 (OTU47_04220) asnA 786735..787727 (+) 993 WP_000747993.1 aspartate--ammonia ligase -
  OTU47_RS04225 (OTU47_04225) rsmD 787792..788331 (+) 540 WP_001819515.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  OTU47_RS04230 (OTU47_04230) coaD 788321..788809 (+) 489 WP_001280769.1 pantetheine-phosphate adenylyltransferase -
  OTU47_RS04235 (OTU47_04235) sepM 788793..789830 (+) 1038 WP_000727277.1 SepM family pheromone-processing serine protease Regulator
  OTU47_RS04240 (OTU47_04240) - 789888..790118 (+) 231 WP_000250384.1 DUF1146 family protein -
  OTU47_RS04245 (OTU47_04245) murA 790142..791425 (+) 1284 WP_000358028.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  OTU47_RS04250 (OTU47_04250) - 791418..791606 (+) 189 WP_050086732.1 DNA-directed RNA polymerase subunit beta -
  OTU47_RS04255 (OTU47_04255) endA 791645..792469 (+) 825 WP_001036784.1 DNA-entry nuclease EndA Machinery gene
  OTU47_RS04260 (OTU47_04260) - 792748..794079 (+) 1332 WP_000390042.1 hemolysin family protein -

Sequence

Protein


Download         Length: 274 a.a.        Molecular weight: 29931.63 Da        Isoelectric Point: 10.1197

>NTDB_id=760420 OTU47_RS04255 WP_001036784.1 791645..792469(+) (endA) [Streptococcus pneumoniae strain NP2]
MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSAPNSPKTNLSQKKQAPEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIV
NGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRVRYRVTLYYASNEDLVPSASQIE
AKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ

Nucleotide


Download         Length: 825 bp        

>NTDB_id=760420 OTU47_RS04255 WP_001036784.1 791645..792469(+) (endA) [Streptococcus pneumoniae strain NP2]
ATGAACAAAAAAACAAGACAGACACTAATCGGACTGCTAGTGTTATTGCTTTTGTCTACAGGGAGCTATTATATCAAGCA
GATGCAGTCGGCACCTAATAGTCCCAAAACCAATCTTAGTCAGAAAAAACAAGCGCCTGAAGCTCCTAGTCAAGCATTGG
CAGAGAGTGTCTTAACAGACGCAGTCAAGAGTCAAATAAAGGGGAGTCTGGAGTGGAATGGCTCAGGTGCTTTTATCGTC
AATGGTAATAAAACAAATCTAGATGCCAAGGTTTCAAGTAAGCCCTACGCTGACAATAAAACAAAGACAGTGGGCAAGGA
AACTGTTCCAACCGTAGCTAATGCCCTCTTGTCTAAGGCCACTCGTCAGTACAAGAATCGTAAAGAAACTGGGAATGGTT
CAACTTCTTGGACTCCTCCAGGTTGGCATCAGGTCAAGAATCTAAAGGGCTCTTATACCCATGCAGTCGATAGAGGTCAT
TTGTTAGGCTATGCCTTAATCGGTGGTTTGGATGGTTTTGATGCCTCAACAAGCAATCCTAAAAACATTGCTGTTCAGAC
AGCCTGGGCAAATCAGGCACAAGCCGAGTATTCGACTGGTCAAAACTACTATGAAAGCAAGGTGCGTAAAGCCTTGGACC
AAAACAAGCGTGTCCGTTACCGTGTAACCCTTTACTACGCTTCAAACGAGGATTTAGTTCCCTCAGCTTCACAGATTGAA
GCCAAGTCTTCGGATGGAGAATTGGAATTCAATGTTCTAGTTCCCAATGTTCAAAAGGGACTTCAACTGGATTACCGAAC
TGGAGAAGTAACTGTAACTCAGTAA

Domains

Predicted by InterproScan.

DNA/RNA non-specific endonuclease (Endonuclease_NS)

(75-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0E7E4I0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

99.27

100

0.993
  endA Streptococcus pneumoniae D39

99.27

100

0.993
  endA Streptococcus pneumoniae R6

99.27

100

0.993
  endA Streptococcus pneumoniae TIGR4

99.27

100

0.993