Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   OTU45_RS10310 Genome accession   NZ_CP113114
Coordinates   2015707..2016531 (-) Length   274 a.a.
NCBI ID   WP_001036784.1    Uniprot ID   A0A0E7E4I0
Organism   Streptococcus pneumoniae strain NP1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2010707..2021531
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OTU45_RS10305 (OTU45_10305) - 2014097..2015428 (-) 1332 WP_000390042.1 hemolysin family protein -
  OTU45_RS10310 (OTU45_10310) endA 2015707..2016531 (-) 825 WP_001036784.1 DNA-entry nuclease EndA Machinery gene
  OTU45_RS10315 (OTU45_10315) - 2016570..2016758 (-) 189 WP_050086732.1 DNA-directed RNA polymerase subunit beta -
  OTU45_RS10320 (OTU45_10320) murA 2016751..2018034 (-) 1284 WP_000358028.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  OTU45_RS10325 (OTU45_10325) - 2018058..2018288 (-) 231 WP_000250384.1 DUF1146 family protein -
  OTU45_RS10330 (OTU45_10330) sepM 2018346..2019383 (-) 1038 WP_000727277.1 SepM family pheromone-processing serine protease Regulator
  OTU45_RS10335 (OTU45_10335) coaD 2019367..2019855 (-) 489 WP_001280769.1 pantetheine-phosphate adenylyltransferase -
  OTU45_RS10340 (OTU45_10340) rsmD 2019845..2020384 (-) 540 WP_001819515.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  OTU45_RS10345 (OTU45_10345) asnA 2020449..2021441 (-) 993 WP_000747993.1 aspartate--ammonia ligase -

Sequence

Protein


Download         Length: 274 a.a.        Molecular weight: 29931.63 Da        Isoelectric Point: 10.1197

>NTDB_id=760365 OTU45_RS10310 WP_001036784.1 2015707..2016531(-) (endA) [Streptococcus pneumoniae strain NP1]
MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSAPNSPKTNLSQKKQAPEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIV
NGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRVRYRVTLYYASNEDLVPSASQIE
AKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ

Nucleotide


Download         Length: 825 bp        

>NTDB_id=760365 OTU45_RS10310 WP_001036784.1 2015707..2016531(-) (endA) [Streptococcus pneumoniae strain NP1]
ATGAACAAAAAAACAAGACAGACACTAATCGGACTGCTAGTGTTATTGCTTTTGTCTACAGGGAGCTATTATATCAAGCA
GATGCAGTCGGCACCTAATAGTCCCAAAACCAATCTTAGTCAGAAAAAACAAGCGCCTGAAGCTCCTAGTCAAGCATTGG
CAGAGAGTGTCTTAACAGACGCAGTCAAGAGTCAAATAAAGGGGAGTCTGGAGTGGAATGGCTCAGGTGCTTTTATCGTC
AATGGTAATAAAACAAATCTAGATGCCAAGGTTTCAAGTAAGCCCTACGCTGACAATAAAACAAAGACAGTGGGCAAGGA
AACTGTTCCAACCGTAGCTAATGCCCTCTTGTCTAAGGCCACTCGTCAGTACAAGAATCGTAAAGAAACTGGGAATGGTT
CAACTTCTTGGACTCCTCCAGGTTGGCATCAGGTCAAGAATCTAAAGGGCTCTTATACCCATGCAGTCGATAGAGGTCAT
TTGTTAGGCTATGCCTTAATCGGTGGTTTGGATGGTTTTGATGCCTCAACAAGCAATCCTAAAAACATTGCTGTTCAGAC
AGCCTGGGCAAATCAGGCACAAGCCGAGTATTCGACTGGTCAAAACTACTATGAAAGCAAGGTGCGTAAAGCCTTGGACC
AAAACAAGCGTGTCCGTTACCGTGTAACCCTTTACTACGCTTCAAACGAGGATTTAGTTCCCTCAGCTTCACAGATTGAA
GCCAAGTCTTCGGATGGAGAATTGGAATTCAATGTTCTAGTTCCCAATGTTCAAAAGGGACTTCAACTGGATTACCGAAC
TGGAGAAGTAACTGTAACTCAGTAA

Domains

Predicted by InterproScan.

DNA/RNA non-specific endonuclease (Endonuclease_NS)

(75-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0E7E4I0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

99.27

100

0.993
  endA Streptococcus pneumoniae D39

99.27

100

0.993
  endA Streptococcus pneumoniae R6

99.27

100

0.993
  endA Streptococcus pneumoniae TIGR4

99.27

100

0.993