Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   P7I93_RS27295 Genome accession   NZ_CP124658
Coordinates   5707540..5707965 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain 2022CK-00068     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5702540..5712965
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P7I93_RS27280 (P7I93_27280) pilX 5703110..5703697 (+) 588 WP_003094700.1 type 4a pilus minor pilin PilX -
  P7I93_RS27285 (P7I93_27285) pilY1 5703709..5707194 (+) 3486 WP_003134937.1 type 4a pilus biogenesis protein PilY1 -
  P7I93_RS27290 (P7I93_27290) pilY2 5707196..5707543 (+) 348 WP_003094713.1 type 4a fimbrial biogenesis protein PilY2 -
  P7I93_RS27295 (P7I93_27295) comF 5707540..5707965 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  P7I93_RS27300 (P7I93_27300) ispH 5708012..5708956 (-) 945 WP_003134939.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  P7I93_RS27305 (P7I93_27305) fkpB 5709042..5709482 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  P7I93_RS27310 (P7I93_27310) lspA 5709475..5709984 (-) 510 WP_003094728.1 signal peptidase II -
  P7I93_RS27315 (P7I93_27315) ileS 5709977..5712808 (-) 2832 WP_023092936.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=760195 P7I93_RS27295 WP_003094721.1 5707540..5707965(+) (comF) [Pseudomonas aeruginosa strain 2022CK-00068]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=760195 P7I93_RS27295 WP_003094721.1 5707540..5707965(+) (comF) [Pseudomonas aeruginosa strain 2022CK-00068]
ATGAGGACAAGACAGAAGGGCTTCACGCTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAACTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383