Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   P7I80_RS28215 Genome accession   NZ_CP124649
Coordinates   5920976..5921401 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain 2020CK-00218     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5915976..5926401
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P7I80_RS28200 (P7I80_28200) pilX 5916540..5917127 (+) 588 WP_003094700.1 type 4a pilus minor pilin PilX -
  P7I80_RS28205 (P7I80_28205) pilY1 5917139..5920630 (+) 3492 WP_023096128.1 type 4a pilus biogenesis protein PilY1 -
  P7I80_RS28210 (P7I80_28210) pilY2 5920632..5920979 (+) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  P7I80_RS28215 (P7I80_28215) comF 5920976..5921401 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  P7I80_RS28220 (P7I80_28220) ispH 5921448..5922392 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  P7I80_RS28225 (P7I80_28225) fkpB 5922478..5922918 (-) 441 WP_003094726.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  P7I80_RS28230 (P7I80_28230) lspA 5922911..5923420 (-) 510 WP_003094728.1 signal peptidase II -
  P7I80_RS28235 (P7I80_28235) ileS 5923413..5926244 (-) 2832 WP_003094730.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=759591 P7I80_RS28215 WP_003094721.1 5920976..5921401(+) (comF) [Pseudomonas aeruginosa strain 2020CK-00218]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=759591 P7I80_RS28215 WP_003094721.1 5920976..5921401(+) (comF) [Pseudomonas aeruginosa strain 2020CK-00218]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383