Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   P7I81_RS06495 Genome accession   NZ_CP124648
Coordinates   1345514..1345939 (+) Length   141 a.a.
NCBI ID   WP_023107845.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 2020CK-00220     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1340514..1350939
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P7I81_RS06480 (P7I81_06480) pilX 1341078..1341665 (+) 588 WP_034001493.1 type 4a pilus minor pilin PilX -
  P7I81_RS06485 (P7I81_06485) pilY1 1341677..1345168 (+) 3492 WP_023111845.1 type 4a pilus biogenesis protein PilY1 -
  P7I81_RS06490 (P7I81_06490) pilY2 1345170..1345517 (+) 348 WP_003094713.1 type 4a fimbrial biogenesis protein PilY2 -
  P7I81_RS06495 (P7I81_06495) comF 1345514..1345939 (+) 426 WP_023107845.1 type 4a pilus minor pilin PilE Machinery gene
  P7I81_RS06500 (P7I81_06500) ispH 1345986..1346930 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  P7I81_RS06505 (P7I81_06505) fkpB 1347016..1347456 (-) 441 WP_003094726.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  P7I81_RS06510 (P7I81_06510) lspA 1347449..1347958 (-) 510 WP_003094728.1 signal peptidase II -
  P7I81_RS06515 (P7I81_06515) ileS 1347951..1350782 (-) 2832 WP_003094730.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15309.33 Da        Isoelectric Point: 10.0198

>NTDB_id=759441 P7I81_RS06495 WP_023107845.1 1345514..1345939(+) (comF) [Pseudomonas aeruginosa strain 2020CK-00220]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVTKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=759441 P7I81_RS06495 WP_023107845.1 1345514..1345939(+) (comF) [Pseudomonas aeruginosa strain 2020CK-00220]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGCTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCGCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGACCAAACTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

41.406

90.78

0.376