Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   P7I83_RS24635 Genome accession   NZ_CP124624
Coordinates   5291198..5291623 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain 2021CK-01157     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5286198..5296623
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P7I83_RS24620 (P7I83_24620) pilX 5286768..5287355 (+) 588 WP_003094700.1 type 4a pilus minor pilin PilX -
  P7I83_RS24625 (P7I83_24625) pilY1 5287367..5290852 (+) 3486 WP_019486630.1 type 4a pilus biogenesis protein PilY1 -
  P7I83_RS24630 (P7I83_24630) pilY2 5290854..5291201 (+) 348 WP_003094713.1 type 4a fimbrial biogenesis protein PilY2 -
  P7I83_RS24635 (P7I83_24635) comF 5291198..5291623 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  P7I83_RS24640 (P7I83_24640) ispH 5291668..5292612 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  P7I83_RS24645 (P7I83_24645) fkpB 5292698..5293138 (-) 441 WP_003094726.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  P7I83_RS24650 (P7I83_24650) lspA 5293131..5293640 (-) 510 WP_003094728.1 signal peptidase II -
  P7I83_RS24655 (P7I83_24655) ileS 5293633..5296464 (-) 2832 WP_003094730.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=758900 P7I83_RS24635 WP_003094721.1 5291198..5291623(+) (comF) [Pseudomonas aeruginosa strain 2021CK-01157]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=758900 P7I83_RS24635 WP_003094721.1 5291198..5291623(+) (comF) [Pseudomonas aeruginosa strain 2021CK-01157]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATTCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCAGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACACCCACCAGCACTACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383