Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   P7I85_RS25050 Genome accession   NZ_CP124622
Coordinates   5367169..5367594 (+) Length   141 a.a.
NCBI ID   WP_282414025.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 2021CK-01159     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5362169..5372594
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P7I85_RS25035 (P7I85_25035) pilX 5362733..5363320 (+) 588 WP_003112826.1 type 4a pilus minor pilin PilX -
  P7I85_RS25040 (P7I85_25040) pilY1 5363332..5366823 (+) 3492 WP_003123397.1 type 4a pilus biogenesis protein PilY1 -
  P7I85_RS25045 (P7I85_25045) pilY2 5366825..5367172 (+) 348 WP_003161774.1 type 4a fimbrial biogenesis protein PilY2 -
  P7I85_RS25050 (P7I85_25050) comF 5367169..5367594 (+) 426 WP_282414025.1 type 4a pilus minor pilin PilE Machinery gene
  P7I85_RS25055 (P7I85_25055) ispH 5367641..5368585 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  P7I85_RS25060 (P7I85_25060) fkpB 5368671..5369111 (-) 441 WP_003161775.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  P7I85_RS25065 (P7I85_25065) lspA 5369104..5369613 (-) 510 WP_003110420.1 signal peptidase II -
  P7I85_RS25070 (P7I85_25070) ileS 5369606..5372437 (-) 2832 WP_257680085.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15337.34 Da        Isoelectric Point: 9.8393

>NTDB_id=758803 P7I85_RS25050 WP_282414025.1 5367169..5367594(+) (comF) [Pseudomonas aeruginosa strain 2021CK-01159]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLDMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=758803 P7I85_RS25050 WP_282414025.1 5367169..5367594(+) (comF) [Pseudomonas aeruginosa strain 2021CK-01159]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGCTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAACTGGACATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACACCCACCAGCACTACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

41.406

90.78

0.376