Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   OP701_RS06000 Genome accession   NZ_CP111073
Coordinates   1250957..1254457 (+) Length   1166 a.a.
NCBI ID   WP_060398327.1    Uniprot ID   -
Organism   Bacillus inaquosorum strain BSXE-2102     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1245957..1259457
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OP701_RS05980 (OP701_05980) - 1246811..1248013 (-) 1203 WP_060398324.1 MDR family MFS transporter -
  OP701_RS05985 (OP701_05985) - 1248127..1249854 (+) 1728 WP_060398325.1 SgrR family transcriptional regulator -
  OP701_RS05990 (OP701_05990) - 1249891..1250220 (-) 330 WP_060398326.1 four-helix bundle copper-binding protein -
  OP701_RS05995 (OP701_05995) - 1250337..1250774 (-) 438 WP_044427329.1 ferritin family protein -
  OP701_RS06000 (OP701_06000) addB 1250957..1254457 (+) 3501 WP_060398327.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  OP701_RS06005 (OP701_06005) addA 1254444..1258148 (+) 3705 WP_060398328.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  OP701_RS06010 (OP701_06010) sbcD 1258220..1259395 (+) 1176 WP_060398329.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 134654.76 Da        Isoelectric Point: 5.6870

>NTDB_id=757641 OP701_RS06000 WP_060398327.1 1250957..1254457(+) (addB) [Bacillus inaquosorum strain BSXE-2102]
MGAEFLVGRSGSGKTKLIIDSIQDELRRAPFGKPIIFLVPDQMTFLMEYELAKTPDMGGMIRAQVFSFSRLAWRVLQHTG
GMSRPFLTSTGVQMLLRKLIEEHKQEFKVYQKASDKSGFTAQVERMLTEFKRYCLEPEDIRRMVESGTASEYRGERILSE
KLHDLSILYQQMEKSLAGQYLHSEDYLTLLAEHVPLAEDVKGAHIYVDGFYQFTPQEFRVLEQLMVHAEHITFSLTADKP
SYEREPHELELFRMTGKTYYRLHQKAKELNLDITYKELSGTERHTRTPELAHLEAQYEARPAVPYAEKQEAFTVMQAANR
RAELEGIAREIHALVREKGYRYKDVAILARQPEDYKDMVKEVFADYEIPYFIDGKASMLNHPLIEFIRSSLDVLKGNWRY
EAVFRCVKTELLFPLKEPKTKVREQVDQLENYCIAYGIKGDRWTKGDRFHYRRFVSLDDDFAQTDQEIEMENMLNDTRDW
IVPPLFQLQKRMKKAKTVQEKAEALYRYLEETDVPLKLDQERQRAEEDGRIIEAQQHQQAWDAVIQLLEEFVEMMGDDEI
SLDLFQQMMEAGAESLTFSLIPPALDQVFVGNMDLSRMYGTSCTFVLGANDGVLPARPDENGVLSDDDREWLKTVGVELS
SGGRERLLDEHFLIYMAFSSPSDRLYVSYPIADAEGKTLLPSIVIKRLEELFPQHKERLLTNEPEQVSDEEQLMYVVNKS
VAQSFTASQLRLWTREYDISDVWWSTYNVLMSEPDRLQSKKLFSSLFFRNEVKQLERSVSRQLYGERIQGSVSRMETFNA
CPFSHFASHGLHLKERQFFKLEAPDIGQLFHSSLKLISDRLREQKMDWRDLTKEQCELFSHDAVERLAPKLQKEILLSSN
RHYYVKEKLQKIVTRVSGILSEHAKASGFVPIGLELGFGGKGPLPPLTFKLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSADWLGMRATPAGVLYFHIHDPMIQSNLPLGLDEIEQEIFKKFKMKGL
LLGDQEAIRLMDTTLQEGRSNIINAGLKKDGSLRSDSAAVGEKEFELLTKHVRRTFQEAGEQITDGRVSIEPYKMKNKTP
CTYCAFKSVCQFDESLEENEYRPLKAEKDKTILEWIKKEADGNEHS

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=757641 OP701_RS06000 WP_060398327.1 1250957..1254457(+) (addB) [Bacillus inaquosorum strain BSXE-2102]
TTGGGAGCAGAGTTTTTAGTAGGCAGGTCCGGGAGTGGAAAAACGAAGCTGATCATCGACAGCATTCAGGATGAACTGCG
CCGGGCTCCATTCGGGAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATACGAGCTTGCTAAAA
CGCCTGATATGGGCGGAATGATACGCGCTCAAGTGTTCAGCTTTTCACGATTGGCCTGGCGCGTCCTCCAGCATACGGGA
GGCATGAGCAGGCCGTTTCTTACGAGCACAGGCGTACAGATGCTCCTGCGGAAGCTCATTGAGGAGCATAAACAGGAGTT
TAAAGTCTATCAAAAAGCGAGTGACAAAAGCGGGTTTACCGCACAGGTAGAACGGATGCTGACAGAGTTTAAGCGCTACT
GTCTGGAACCGGAAGACATCCGCCGGATGGTGGAAAGCGGAACGGCTTCCGAGTATCGCGGAGAACGTATTTTATCTGAG
AAGCTTCATGATTTATCGATTTTATATCAACAGATGGAAAAAAGCCTTGCAGGTCAATACCTTCACTCTGAGGATTATTT
AACATTGCTGGCAGAGCATGTTCCGCTTGCAGAAGATGTAAAAGGCGCGCATATCTATGTGGACGGCTTTTATCAGTTTA
CACCGCAGGAATTCAGGGTGTTGGAGCAGCTTATGGTTCATGCAGAGCACATCACGTTTTCGCTGACAGCGGACAAGCCG
TCATATGAGCGGGAGCCGCATGAACTGGAATTGTTCAGAATGACGGGAAAAACCTATTACCGCCTGCACCAAAAGGCGAA
GGAACTGAATCTGGACATTACGTATAAAGAGCTAAGCGGGACTGAACGGCATACGCGCACACCGGAATTGGCACATTTAG
AGGCGCAGTATGAAGCGCGTCCAGCCGTTCCGTACGCAGAAAAACAAGAGGCCTTTACTGTGATGCAGGCCGCAAACAGA
CGAGCTGAGCTGGAAGGCATTGCCCGGGAAATTCACGCTCTTGTCAGAGAGAAGGGCTATCGCTATAAGGATGTAGCGAT
TCTTGCGCGCCAGCCGGAAGACTACAAGGATATGGTGAAGGAAGTTTTCGCAGATTACGAGATTCCTTATTTCATTGACG
GAAAAGCATCAATGCTGAACCATCCGTTAATCGAATTTATCCGGTCGAGCCTTGATGTTTTGAAAGGGAATTGGCGTTAT
GAAGCAGTGTTTCGCTGCGTGAAAACCGAACTGCTATTCCCGCTTAAAGAGCCGAAGACTAAGGTGAGAGAACAGGTCGA
TCAGCTTGAAAATTACTGTATCGCCTACGGGATCAAAGGTGACCGCTGGACAAAGGGCGACCGTTTCCATTACAGACGTT
TTGTGTCATTGGATGATGATTTTGCGCAGACCGACCAGGAAATCGAAATGGAAAACATGCTGAATGACACACGCGACTGG
ATTGTTCCTCCGCTTTTTCAGCTCCAGAAACGAATGAAAAAAGCAAAGACGGTTCAGGAGAAGGCGGAGGCGCTCTACCG
CTATCTAGAAGAGACGGATGTGCCGCTGAAGCTGGATCAGGAAAGACAGCGTGCAGAGGAAGACGGCAGAATCATTGAAG
CACAGCAGCATCAGCAGGCGTGGGATGCAGTCATTCAGCTGCTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATT
TCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCAGAGTCGCTTACATTTTCTCTTATTCCGCCTGCGCTTGACCA
GGTGTTTGTCGGCAATATGGATTTGTCCAGAATGTATGGCACCTCCTGTACCTTTGTGCTGGGGGCAAACGACGGCGTTC
TGCCGGCGCGCCCAGATGAAAACGGAGTGCTGTCGGATGATGACCGGGAATGGCTGAAAACGGTTGGGGTTGAGCTTTCC
TCAGGCGGGCGGGAGCGTCTGCTTGATGAGCATTTCCTCATCTATATGGCGTTTTCCAGTCCGTCTGACCGGCTGTACGT
ATCGTATCCGATTGCTGATGCGGAAGGAAAAACGCTTCTGCCGTCGATTGTCATTAAGCGGCTGGAAGAACTGTTTCCGC
AGCATAAGGAGCGCCTGTTGACAAATGAACCTGAGCAGGTCAGCGATGAGGAACAGCTGATGTATGTCGTCAATAAAAGC
GTGGCACAGTCCTTTACCGCGAGCCAGCTCAGGTTATGGACTCGGGAATACGACATCAGCGACGTCTGGTGGAGCACGTA
CAATGTGTTGATGAGTGAGCCGGACAGGCTGCAATCGAAAAAGTTATTCTCAAGCCTGTTTTTCCGGAATGAAGTGAAGC
AGCTTGAACGCAGCGTGTCGAGACAGCTCTATGGTGAACGCATTCAGGGCAGTGTATCGAGAATGGAAACCTTTAACGCG
TGCCCGTTCTCCCATTTTGCGTCACACGGGCTGCATCTGAAGGAACGGCAATTTTTCAAGCTCGAAGCACCGGATATCGG
CCAGCTGTTTCATTCCAGCTTAAAGCTGATTTCAGACCGGCTGCGCGAGCAAAAAATGGATTGGCGCGATTTAACGAAAG
AGCAGTGCGAGCTGTTCTCCCATGATGCGGTAGAGCGTCTGGCGCCGAAACTGCAAAAGGAAATTTTGCTCAGCTCAAAC
CGGCATTATTATGTGAAGGAAAAACTGCAAAAAATTGTGACACGCGTGTCCGGCATTTTAAGCGAGCATGCGAAAGCGAG
CGGTTTCGTGCCGATCGGGCTTGAACTGGGCTTTGGAGGAAAAGGGCCGCTTCCGCCGCTGACCTTTAAGCTGAAAAACG
GCTGCACGATGGAGCTCGTCGGGCGAATTGACCGCGTAGATAAAGCGGAAAGCTCAAAAGGCCTGCTTCTAAGAATTGTC
GATTATAAATCAAGCGACAAAGGCCTTGACTTAGCGGAAGTATATTACGGACTGGCGCTGCAAATGCTGACGTACCTTGA
TTTATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGAGCGACGCCGGCCGGAGTGCTGTATTTCCATATTCATGACC
CGATGATTCAATCTAACCTCCCGCTTGGGCTTGACGAGATCGAACAGGAGATCTTTAAGAAATTTAAGATGAAGGGCCTG
CTCCTCGGCGATCAGGAAGCGATTCGCCTCATGGATACGACTCTTCAAGAGGGACGGTCAAATATCATCAACGCCGGCTT
GAAAAAAGACGGCTCTCTCAGATCGGACTCGGCGGCAGTCGGCGAAAAGGAATTTGAGCTGCTGACGAAGCATGTGCGCC
GCACCTTCCAAGAAGCAGGCGAACAAATCACCGACGGACGCGTATCGATTGAGCCTTATAAAATGAAGAACAAGACACCG
TGCACATACTGTGCGTTCAAATCAGTATGCCAATTTGATGAATCATTAGAAGAAAACGAGTATCGCCCGTTAAAGGCTGA
AAAGGACAAGACGATACTTGAGTGGATAAAAAAGGAGGCGGATGGCAATGAACATTCCTAA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(306-615)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

97.77

100

0.978