Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   OHU65_RS23385 Genome accession   NZ_CP109859
Coordinates   4460968..4463310 (-) Length   780 a.a.
NCBI ID   WP_264408586.1    Uniprot ID   -
Organism   Priestia megaterium strain 207     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4455968..4468310
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OHU65_RS23360 (OHU65_23360) - 4456361..4456537 (-) 177 WP_013059305.1 hypothetical protein -
  OHU65_RS23365 (OHU65_23365) - 4456704..4457588 (+) 885 WP_260506748.1 N-acetylmuramoyl-L-alanine amidase -
  OHU65_RS23370 (OHU65_23370) dtd 4457579..4458040 (-) 462 WP_221784616.1 D-aminoacyl-tRNA deacylase -
  OHU65_RS23375 (OHU65_23375) - 4458056..4460254 (-) 2199 WP_014458138.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  OHU65_RS23380 (OHU65_23380) - 4460440..4460952 (-) 513 WP_013059309.1 adenine phosphoribosyltransferase -
  OHU65_RS23385 (OHU65_23385) recJ 4460968..4463310 (-) 2343 WP_264408586.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  OHU65_RS23390 (OHU65_23390) secDF 4463452..4465689 (-) 2238 WP_257041500.1 protein translocase subunit SecDF -
  OHU65_RS23395 (OHU65_23395) - 4465827..4466120 (-) 294 WP_025750585.1 post-transcriptional regulator -
  OHU65_RS23400 (OHU65_23400) spoVB 4466185..4467795 (+) 1611 WP_264408587.1 stage V sporulation protein B -

Sequence

Protein


Download         Length: 780 a.a.        Molecular weight: 87962.61 Da        Isoelectric Point: 4.7460

>NTDB_id=750283 OHU65_RS23385 WP_264408586.1 4460968..4463310(-) (recJ) [Priestia megaterium strain 207]
MLQSKKRWNVQECDEQLVNEFVENLNITPLVASLLLNRGMTTVEAAREFLHVDAQTFHDPFLLHDMDKAVARIQQAIDHN
EKIMIYGDYDADGVSSTSVMLSALQQLGADVDFYIPNRFTEGYGPNKAAFDKISAGGYQLVITVDTGISAVEETAYAREL
NFDLIITDHHEPGPVLPDAYAIIHPKLPGSTYPFKELAGVGVAFKLAHALLGELPEHLLELAVIGTIADLVPLVDENRLI
ARKGLDYLKLTKRTGIEALLSVCGVKREEINEDTIGFAMAPRINAVGRLQDADPAVHLLMTTNAEEAKGLAKEIDALNKE
RQQIVNDITEEAVEFVEDMYPPDESSVLIVEGEGWNVGVVGIVASRLVEKFYRPTIVLSIDSENRIAKGSARSIEGFDLF
ANLSKCRDILPHFGGHPMAAGMTLNSDDVEELRRRLNEQASNVLTQQDFIPITSVDTVCTTEEITLENIEQMSALAPFGM
HNPKPKVLIKDVHTTSMRKIGANKNHLKLVFEDSGVSVDAVGFGLGYILDEVSPIAKVSLVGELSINEWNNMRKPQLMVE
DISVDEWQMFDYRSAVDVKRFFQQVFTDQMKVVAFHASTIDKYKSLLPMEHVVLIQNEEQAKACVFAEEQIVLLDLPPSQ
KWLECLFVQGIPFRIYAIFLTEEDHYFDTIPTRDHFKWFYGFLAKKGPFDLKRYKEDLAIHKGWSKETIDFMAKVFFELE
FVTINNGLISLVKNSAKRDLIESATYRQKQQQIEIEKNFIYTSYVQLKNQFQDIFKRSIE

Nucleotide


Download         Length: 2343 bp        

>NTDB_id=750283 OHU65_RS23385 WP_264408586.1 4460968..4463310(-) (recJ) [Priestia megaterium strain 207]
ATGTTACAGTCAAAAAAACGTTGGAATGTTCAAGAATGTGATGAACAACTTGTTAATGAATTTGTAGAAAACCTCAATAT
TACACCTCTAGTAGCTTCGTTATTACTTAATAGAGGCATGACAACAGTAGAAGCGGCGCGTGAGTTTTTACACGTTGATG
CACAAACGTTCCACGATCCGTTTTTATTGCACGATATGGACAAAGCAGTAGCTCGTATTCAGCAAGCAATTGATCATAAT
GAAAAAATTATGATCTATGGAGATTACGATGCTGACGGCGTCAGCAGCACTTCTGTTATGTTATCTGCTTTACAGCAGCT
TGGCGCTGATGTTGATTTTTATATTCCTAATCGCTTTACAGAAGGTTATGGGCCGAATAAGGCGGCATTTGATAAAATAA
GCGCAGGCGGCTATCAGCTGGTGATTACGGTAGACACAGGTATTTCGGCAGTCGAAGAAACTGCGTATGCACGTGAACTA
AACTTTGATTTAATTATTACTGATCACCACGAGCCGGGGCCGGTTTTACCGGATGCTTATGCAATTATTCATCCAAAGCT
GCCGGGAAGTACATATCCATTTAAAGAACTGGCAGGTGTAGGAGTCGCTTTTAAACTCGCTCATGCGCTGTTAGGGGAGC
TTCCAGAACATTTGTTAGAACTAGCAGTAATTGGGACGATTGCTGACTTGGTTCCTTTAGTCGACGAAAACCGCTTAATT
GCTCGCAAAGGTCTTGACTATCTCAAGCTCACGAAGCGCACCGGAATTGAAGCGCTGCTGAGCGTATGTGGAGTAAAACG
TGAAGAAATTAATGAGGATACAATCGGATTTGCGATGGCTCCTCGTATTAATGCAGTAGGACGTCTCCAAGATGCCGATC
CAGCTGTTCATTTGCTTATGACGACAAATGCTGAAGAGGCAAAAGGTTTAGCTAAAGAAATTGATGCGTTAAACAAGGAA
AGGCAGCAAATCGTAAACGATATTACAGAAGAAGCCGTTGAATTTGTGGAAGATATGTATCCTCCGGATGAAAGCAGCGT
TCTTATTGTAGAGGGAGAAGGATGGAATGTAGGCGTTGTTGGAATTGTCGCTTCTAGGCTCGTAGAAAAGTTCTACAGAC
CAACAATTGTATTAAGTATTGACTCTGAAAATCGTATTGCTAAAGGTTCAGCAAGAAGTATTGAAGGCTTTGACTTATTC
GCGAACTTATCTAAATGCCGTGACATTCTGCCGCATTTTGGAGGGCACCCAATGGCAGCCGGCATGACATTAAACAGCGA
TGATGTAGAAGAACTGCGCAGACGCCTTAATGAACAAGCAAGCAACGTGTTAACTCAACAGGATTTTATTCCAATTACAT
CAGTTGATACAGTATGCACCACAGAGGAAATCACGCTGGAAAACATTGAACAAATGAGTGCGCTTGCTCCATTTGGTATG
CATAACCCGAAGCCAAAAGTTCTCATTAAAGATGTTCATACAACATCTATGCGGAAAATTGGTGCTAATAAAAATCATTT
AAAACTTGTATTTGAAGACAGCGGTGTATCGGTTGATGCAGTAGGATTTGGGTTAGGCTATATATTAGATGAAGTATCTC
CTATAGCTAAAGTATCATTAGTAGGTGAACTATCTATTAATGAGTGGAACAATATGCGAAAGCCGCAGCTGATGGTGGAA
GATATTTCTGTAGATGAATGGCAGATGTTTGATTATCGCAGTGCAGTTGATGTCAAACGTTTCTTTCAGCAGGTGTTTAC
GGATCAAATGAAAGTTGTTGCTTTTCACGCTTCAACAATTGATAAATACAAGTCGCTGCTGCCGATGGAGCATGTTGTTC
TTATTCAAAATGAAGAACAGGCAAAAGCATGTGTGTTTGCAGAGGAACAGATCGTTTTATTAGATTTGCCTCCTTCACAA
AAGTGGCTTGAATGTTTATTTGTTCAAGGTATCCCTTTTAGAATATACGCTATTTTCTTAACGGAAGAAGACCATTATTT
TGATACAATCCCAACTCGCGATCATTTTAAATGGTTTTACGGCTTTTTAGCGAAAAAGGGTCCATTTGATTTAAAACGCT
ACAAAGAGGATTTAGCGATTCATAAGGGCTGGTCAAAAGAAACGATTGATTTTATGGCAAAGGTGTTTTTTGAGTTAGAA
TTTGTTACAATAAACAATGGCTTAATTTCGTTAGTGAAAAATTCTGCGAAACGTGATTTAATAGAATCTGCGACTTATCG
CCAAAAGCAGCAGCAGATTGAAATAGAAAAAAACTTTATTTATACGTCATATGTACAGCTTAAAAATCAATTTCAAGATA
TTTTTAAGCGTTCGATTGAATGA

Domains

Predicted by InterproScan.

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-770)

DHHA1 domain (DHHA1)

(305-439)

RecJ OB domain (RecJ_OB)

(455-561)

DHH family (DHH)

(82-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

55.513

100

0.555