Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   N8A73_RS12265 Genome accession   NZ_CP109651
Coordinates   2383387..2385711 (-) Length   774 a.a.
NCBI ID   WP_254001381.1    Uniprot ID   -
Organism   Bacillus safensis strain LG01     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2378387..2390711
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N8A73_RS12250 dtd 2380037..2380480 (-) 444 WP_024427859.1 D-aminoacyl-tRNA deacylase -
  N8A73_RS12255 - 2380499..2382709 (-) 2211 WP_034279902.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  N8A73_RS12260 - 2382851..2383363 (-) 513 WP_034279900.1 adenine phosphoribosyltransferase -
  N8A73_RS12265 recJ 2383387..2385711 (-) 2325 WP_254001381.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  N8A73_RS12270 - 2385793..2386143 (-) 351 WP_034279896.1 lipopolysaccharide assembly LapA domain-containing protein -
  N8A73_RS12275 - 2386245..2386733 (-) 489 WP_041115109.1 cation:proton antiporter regulatory subunit -
  N8A73_RS12280 secDF 2386920..2389148 (-) 2229 WP_034279892.1 protein translocase subunit SecDF -
  N8A73_RS12285 - 2389190..2389486 (-) 297 WP_003216788.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 774 a.a.        Molecular weight: 87014.66 Da        Isoelectric Point: 4.9495

>NTDB_id=748752 N8A73_RS12265 WP_254001381.1 2383387..2385711(-) (recJ) [Bacillus safensis strain LG01]
MLLSKMRWEYEHPSEEKVKSLSENLNISALTASLLVKRGIEEVEEARSFLFDQKAEFHDPFLLKGMKEAVERINKAIEEQ
ESIVIFGDYDADGVTSTSVLLHTLKERSAIVDFYIPDRFKEGYGPNEQAFRYIKEQGASLVITVDTGIAAVNEARIAKEI
GLDLIITDHHEPSEELPEALAIVHPKQPGCEYPFKELAGVGVAFKVAHALLGKLPVQLLDLAAIGTIADLVPLHDENRWL
AKKGLVQLRQSNRPGLKALLKEAGATLEEANEETVGFQIAPRLNAVGRIEQADPAVHLLMTEDKDEAEELARFVQELNKE
RQKIVSTITEEAIQMVEEAGDDQSAIVVAKAGWNPGVVGIVASKLTDTFSRPAIVLGIDEETQMAKGSARSIPGFDLFFH
LSKCRDILPHFGGHPMAAGMTLRADDVALLRERLNQYANETLTEEDFIPIQRVDAVCKVDELTVEAIEEVGMLSPFGMSN
PKPFIMIEDAKLEDIRTIGANHNHIKMSLKDEDKLLDCVGFHQGKLKEEIVPGSRISVIGEMSVNEWNNRKKPQLMLKDA
RVDEWQLFDLRGKKDWEKKVSTLDSEKSLVVCFDEATKQDVDIPIHHVHAENVSTFDVQGKYVVFADVPADEQLLKQLLE
NQKPARIYTVFQRKEDHFMSSFPSRDQFKWFYGFLFKRGSFPIKEQGMDLAKHRGWTKDTIIFMTKVFFELGFVKIENGV
LSIVLDAPKKDLTASASYTAKQRLMELDQTLTYSSAKELKEWLNSMMSEVSPVL

Nucleotide


Download         Length: 2325 bp        

>NTDB_id=748752 N8A73_RS12265 WP_254001381.1 2383387..2385711(-) (recJ) [Bacillus safensis strain LG01]
ATGTTATTATCCAAAATGCGCTGGGAATATGAACATCCAAGCGAAGAAAAAGTGAAATCACTCTCTGAAAATCTGAATAT
TTCAGCGCTGACTGCATCGCTTCTCGTAAAAAGAGGAATTGAAGAAGTAGAAGAGGCGAGGTCTTTTTTATTTGATCAAA
AAGCCGAATTTCATGATCCATTTCTATTAAAAGGAATGAAAGAAGCAGTAGAGCGCATCAACAAAGCGATCGAAGAGCAG
GAATCGATTGTGATCTTCGGAGACTATGATGCAGATGGTGTGACAAGTACGTCTGTACTTTTACATACATTAAAAGAGCG
GTCTGCGATAGTAGACTTTTATATTCCAGATCGATTTAAAGAAGGCTATGGGCCTAATGAACAGGCATTTCGATATATCA
AGGAGCAAGGAGCATCGCTTGTCATCACGGTCGATACAGGCATTGCTGCTGTAAATGAAGCACGTATTGCAAAAGAAATA
GGGTTAGACCTAATCATTACAGATCACCATGAACCAAGTGAGGAGCTGCCTGAGGCACTTGCTATTGTCCATCCTAAGCA
GCCTGGCTGTGAGTATCCCTTTAAAGAACTTGCTGGTGTAGGCGTAGCGTTTAAAGTGGCGCATGCACTGCTTGGGAAGC
TGCCAGTTCAGCTGTTAGATCTTGCAGCAATCGGGACAATTGCGGACCTTGTACCGCTTCATGATGAGAATAGGTGGCTC
GCCAAAAAAGGCCTTGTGCAGCTTCGGCAGTCGAATAGACCGGGGCTGAAAGCGTTATTGAAAGAAGCGGGAGCCACTCT
AGAAGAAGCCAATGAGGAAACGGTCGGCTTTCAAATTGCGCCAAGGCTGAATGCTGTAGGACGAATTGAACAGGCAGATC
CTGCCGTTCATCTTCTGATGACTGAGGATAAGGATGAAGCAGAAGAGCTCGCTCGTTTTGTACAGGAACTGAATAAAGAA
CGACAAAAGATTGTCAGTACGATCACAGAAGAAGCCATTCAAATGGTGGAAGAGGCAGGTGATGACCAGTCTGCGATCGT
TGTAGCGAAAGCTGGCTGGAATCCGGGCGTTGTCGGAATTGTTGCCTCTAAATTGACGGATACTTTTTCTCGTCCGGCGA
TTGTGCTTGGAATAGATGAAGAGACGCAAATGGCAAAAGGATCAGCCCGGAGTATTCCTGGCTTCGATCTATTTTTCCAT
CTAAGCAAATGCCGCGACATCCTGCCGCATTTCGGGGGGCACCCGATGGCAGCAGGGATGACACTTCGAGCGGATGATGT
CGCTTTACTAAGGGAAAGATTAAATCAATATGCAAATGAAACATTGACGGAAGAAGATTTTATTCCAATACAGCGGGTCG
ATGCAGTATGCAAGGTAGACGAATTGACGGTTGAAGCGATTGAAGAAGTGGGGATGCTGTCACCATTCGGTATGTCAAAC
CCAAAACCGTTCATTATGATTGAAGATGCGAAGCTTGAAGATATTCGAACGATTGGGGCAAATCACAACCATATTAAAAT
GTCTCTGAAAGACGAAGACAAGCTGCTTGACTGTGTCGGTTTTCACCAAGGGAAATTAAAAGAAGAAATTGTTCCAGGAA
GCCGCATTTCCGTGATTGGAGAAATGTCCGTCAATGAATGGAATAACCGGAAGAAGCCCCAGCTGATGCTAAAAGATGCA
CGAGTTGATGAATGGCAGCTGTTTGATCTGCGCGGGAAAAAGGATTGGGAGAAGAAAGTCTCGACGCTTGATTCAGAAAA
ATCTTTGGTTGTCTGTTTTGATGAAGCAACAAAACAGGACGTCGATATCCCCATTCATCATGTTCATGCAGAAAATGTAT
CAACGTTTGATGTACAGGGCAAATATGTGGTCTTTGCGGATGTTCCCGCAGATGAGCAATTGCTGAAGCAGCTTTTAGAA
AATCAAAAGCCTGCCAGAATTTATACCGTTTTTCAAAGAAAAGAAGATCATTTTATGTCCTCTTTCCCAAGCCGTGACCA
ATTCAAATGGTTTTATGGGTTCTTGTTTAAACGGGGAAGTTTTCCTATCAAAGAACAAGGAATGGACCTTGCCAAACATA
GAGGCTGGACAAAAGATACAATCATTTTTATGACAAAGGTGTTTTTTGAACTCGGTTTTGTTAAAATAGAGAATGGTGTG
CTGTCAATAGTACTTGATGCCCCTAAAAAAGATTTAACGGCATCAGCATCCTATACAGCAAAACAACGATTGATGGAACT
CGATCAAACACTAACATATTCCTCAGCTAAAGAGCTGAAGGAATGGCTGAATAGCATGATGAGCGAAGTCTCACCTGTGC
TTTGA

Domains

Predicted by InterproScan.

DHH family (DHH)

(83-226)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-763)

DHHA1 domain (DHHA1)

(311-437)

RecJ OB domain (RecJ_OB)

(453-559)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

67.439

100

0.677