Detailed information NTG0740402

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: OH528_RS08865
• Genome accession: NZ_CP107518
• Coordinates: 1771073..1771873 (+)
• Length: 266 a.a.
• NCBI ID: WP_002888947.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain CRKP_1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1766073..1776873

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OH528_RS08830	gspH	1766229..1766744 (+)	516	WP_004151939.1	type II secretion system minor pseudopilin GspH	-
OH528_RS08835	gspI	1766741..1767106 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
OH528_RS08840	gspJ	1767103..1767699 (+)	597	WP_004151940.1	type II secretion system minor pseudopilin GspJ	-
OH528_RS08845	gspK	1767696..1768682 (+)	987	WP_004151941.1	type II secretion system minor pseudopilin GspK	-
OH528_RS08850	gspL	1768669..1769862 (+)	1194	WP_004151942.1	type II secretion system protein GspL	-
OH528_RS08855	-	1769855..1770340 (+)	486	WP_004151943.1	type II secretion system protein M	-
OH528_RS08860	-	1770330..1771076 (+)	747	WP_004145897.1	type II secretion system protein N	-
OH528_RS08865	pilD	1771073..1771873 (+)	801	WP_002888947.1	prepilin peptidase	Machinery gene
OH528_RS08870	hrpB	1771921..1774350 (-)	2430	WP_004151944.1	ATP-dependent helicase HrpB	-
OH528_RS08875	thpR	1774423..1774959 (+)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
OH528_RS08880	sfsA	1774959..1775675 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
OH528_RS08885	dksA	1775838..1776293 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29419.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=740402 OH528_RS08865 WP_002888947.1 1771073..1771873(+) (pilD) [Klebsiella pneumoniae strain CRKP_1]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGSIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=740402 OH528_RS08865 WP_002888947.1 1771073..1771873(+) (pilD) [Klebsiella pneumoniae strain CRKP_1]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTAGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGA
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGAGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425