Detailed information NTG0740378

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: OH512_RS08845
• Genome accession: NZ_CP107513
• Coordinates: 1766558..1767358 (+)
• Length: 266 a.a.
• NCBI ID: WP_002888947.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain CRKP_2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1761558..1772358

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OH512_RS08810	gspH	1761714..1762229 (+)	516	WP_004151939.1	type II secretion system minor pseudopilin GspH	-
OH512_RS08815	gspI	1762226..1762591 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
OH512_RS08820	gspJ	1762588..1763184 (+)	597	WP_004151940.1	type II secretion system minor pseudopilin GspJ	-
OH512_RS08825	gspK	1763181..1764167 (+)	987	WP_004151941.1	type II secretion system minor pseudopilin GspK	-
OH512_RS08830	gspL	1764154..1765347 (+)	1194	WP_004151942.1	type II secretion system protein GspL	-
OH512_RS08835	-	1765340..1765825 (+)	486	WP_004151943.1	type II secretion system protein M	-
OH512_RS08840	-	1765815..1766561 (+)	747	WP_004145897.1	type II secretion system protein N	-
OH512_RS08845	pilD	1766558..1767358 (+)	801	WP_002888947.1	prepilin peptidase	Machinery gene
OH512_RS08850	hrpB	1767406..1769835 (-)	2430	WP_004151944.1	ATP-dependent helicase HrpB	-
OH512_RS08855	thpR	1769908..1770444 (+)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
OH512_RS08860	sfsA	1770444..1771160 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
OH512_RS08865	dksA	1771323..1771778 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29419.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=740378 OH512_RS08845 WP_002888947.1 1766558..1767358(+) (pilD) [Klebsiella pneumoniae strain CRKP_2]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGSIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=740378 OH512_RS08845 WP_002888947.1 1766558..1767358(+) (pilD) [Klebsiella pneumoniae strain CRKP_2]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTAGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGA
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGAGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425