Detailed information NTG0074017

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: K5658_RS18190
• Genome accession: NZ_AP019783
• Coordinates: 4044927..4045988 (-)
• Length: 353 a.a.
• NCBI ID: WP_221064506.1
• Uniprot ID: -
• Organism: Methylomagnum ishizawai strain RS11D-Pr
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4039927..4050988

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K5658_RS18165 (MishRS11D_36030)	aroK	4040150..4040689 (-)	540	WP_221064502.1	shikimate kinase AroK	-
K5658_RS18170 (MishRS11D_36040)	-	4040810..4043059 (-)	2250	WP_246628488.1	type IV pilus secretin PilQ	-
K5658_RS18175 (MishRS11D_36050)	-	4043056..4043526 (-)	471	WP_246628489.1	pilus assembly protein PilP	-
K5658_RS18180 (MishRS11D_36060)	-	4043636..4044313 (-)	678	WP_221064504.1	type 4a pilus biogenesis protein PilO	-
K5658_RS18185 (MishRS11D_36070)	-	4044310..4044927 (-)	618	WP_221064505.1	PilN domain-containing protein	-
K5658_RS18190 (MishRS11D_36080)	pilM	4044927..4045988 (-)	1062	WP_221064506.1	pilus assembly protein PilM	Machinery gene
K5658_RS18195 (MishRS11D_36100)	-	4046221..4048611 (+)	2391	WP_246628490.1	penicillin-binding protein 1A	-
K5658_RS18200 (MishRS11D_36110)	-	4048619..4049227 (+)	609	WP_221064507.1	hypothetical protein	-
K5658_RS18205 (MishRS11D_36120)	rpmE	4049300..4049503 (-)	204	WP_085211266.1	50S ribosomal protein L31	-
K5658_RS18210 (MishRS11D_36130)	-	4049656..4050435 (+)	780	WP_221064508.1	exodeoxyribonuclease III	-

Sequence

Protein

• Length: 353 a.a.
• Molecular weight: 38263.81 Da
• Isoelectric Point: 5.0247

>NTDB_id=74017 K5658_RS18190 WP_221064506.1 4044927..4045988(-) (pilM) [Methylomagnum ishizawai strain RS11D-Pr]
MFFLNRKKPELLGIDISTAAIKLLELSRSGPRFRVESYAVAPLPPDAVVDKNIAKVEAIGNVIKAVLKQSGTRLKHAAVA
VPGSAVIAKVISMPASLSEDELEAQLELEADQYIPYSLDEVSLDFVVQGVTEKNPEMVDVLLVASRKENVGDRVAALELA
GLKAEIVDVESYALEHSCSLILDQLFERGTDQAIAIADVGATMTTLNVIHNRRIIYTREQGFGGKQLTEEIQRRYGLSYE
EAGLAKKHGGLPDNYGPEVLEPFKNALAQQIGRSLQFFLSSSAHRSVEHILLAGGCASIPGIDRYVEENQGIPTSVANPF
VNMTLSPRVKPQNLSNDAPALMTACGLALRSFD

Nucleotide

• Length: 1062 bp

>NTDB_id=74017 K5658_RS18190 WP_221064506.1 4044927..4045988(-) (pilM) [Methylomagnum ishizawai strain RS11D-Pr]
ATGTTTTTTCTGAACCGCAAGAAGCCGGAGTTATTGGGCATAGACATCAGTACAGCCGCCATCAAACTGTTGGAACTCAG
CCGCTCCGGCCCGCGCTTCCGGGTCGAAAGCTACGCGGTGGCTCCCTTGCCCCCCGACGCGGTGGTCGATAAAAACATCG
CCAAGGTCGAAGCCATCGGCAACGTCATCAAGGCGGTGTTGAAACAATCCGGTACCCGCCTGAAGCACGCGGCGGTGGCG
GTACCGGGCTCCGCCGTGATCGCCAAGGTCATTTCCATGCCCGCCTCCCTGAGCGAGGACGAACTGGAAGCCCAGCTCGA
ATTGGAAGCCGACCAATACATCCCATACTCCCTGGATGAGGTTAGCTTGGATTTCGTGGTCCAGGGCGTCACCGAGAAAA
ATCCCGAGATGGTCGATGTCTTGCTGGTCGCCTCGCGGAAGGAAAATGTCGGCGACCGGGTGGCGGCCTTGGAACTGGCC
GGGCTCAAGGCCGAGATCGTCGATGTCGAATCCTACGCCCTGGAACACTCCTGTTCCCTGATCCTGGACCAACTCTTCGA
GCGCGGCACCGACCAGGCCATCGCCATCGCCGATGTCGGCGCCACCATGACCACGCTCAACGTCATCCACAACCGCCGCA
TCATCTACACCCGCGAACAAGGCTTCGGCGGCAAGCAACTCACCGAGGAAATCCAGCGCCGCTACGGCCTGTCCTACGAG
GAAGCGGGCCTCGCCAAGAAACACGGCGGCCTCCCCGACAACTACGGGCCCGAAGTCCTCGAACCCTTCAAGAACGCCCT
GGCCCAGCAGATCGGGCGCTCCCTGCAATTCTTCCTCTCCTCCAGCGCCCATCGCAGCGTCGAGCATATCCTCCTGGCCG
GCGGTTGCGCCTCGATACCGGGTATCGACCGCTATGTCGAGGAAAACCAAGGCATCCCCACCAGCGTCGCCAATCCCTTC
GTCAATATGACGCTGTCGCCGCGGGTGAAACCGCAGAATTTGAGCAACGACGCGCCCGCGCTGATGACGGCGTGCGGCTT
GGCGCTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (13-352)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	53.295	98.867	0.527
comM	Acinetobacter nosocomialis M2	53.009	98.867	0.524
comM	Acinetobacter baylyi ADP1	50	98.584	0.493
pilM	Legionella pneumophila strain ERS1305867	45.299	99.433	0.45