Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilK   Type   Machinery gene
Locus tag   OCL46_RS02355 Genome accession   NZ_CP106811
Coordinates   453835..454446 (+) Length   203 a.a.
NCBI ID   WP_033911077.1    Uniprot ID   -
Organism   Neisseria gonorrhoeae strain 10531     
Function   type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 440118..493546 453835..454446 within 0


Gene organization within MGE regions


Location: 440118..493546
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCL46_RS02285 (OCL46_02285) - 440118..441101 (+) 984 WP_003687900.1 ribose-phosphate pyrophosphokinase -
  OCL46_RS02290 (OCL46_02290) - 441168..441740 (+) 573 WP_003687901.1 50S ribosomal protein L25/general stress protein Ctc -
  OCL46_RS02295 (OCL46_02295) - 441866..443035 (-) 1170 WP_003687902.1 D-alanyl-D-alanine carboxypeptidase family protein -
  OCL46_RS02300 (OCL46_02300) ilvA 443184..444710 (+) 1527 WP_229930545.1 threonine ammonia-lyase, biosynthetic -
  OCL46_RS02305 (OCL46_02305) - 444766..445842 (-) 1077 WP_003692814.1 sulfate/molybdate ABC transporter ATP-binding protein -
  OCL46_RS02310 (OCL46_02310) cysW 445839..446699 (-) 861 WP_003699617.1 sulfate ABC transporter permease subunit CysW -
  OCL46_RS02315 (OCL46_02315) cysT 446888..447715 (-) 828 WP_003699619.1 sulfate ABC transporter permease subunit CysT -
  OCL46_RS02320 (OCL46_02320) - 447896..448228 (+) 333 WP_003687908.1 hypothetical protein -
  OCL46_RS02325 (OCL46_02325) - 448555..449064 (+) 510 WP_003687909.1 isoprenylcysteine carboxyl methyltransferase family protein -
  OCL46_RS02330 (OCL46_02330) - 449296..449877 (-) 582 WP_003690895.1 superoxide dismutase -
  OCL46_RS02335 (OCL46_02335) dnaB 450041..451447 (+) 1407 WP_003699622.1 replicative DNA helicase -
  OCL46_RS02340 (OCL46_02340) pilH 451601..452266 (+) 666 WP_003699624.1 Tfp pilus assembly protein FimT/FimU Machinery gene
  OCL46_RS02345 (OCL46_02345) pilV 452298..452909 (+) 612 WP_003699626.1 type IV pilus modification protein PilV Machinery gene
  OCL46_RS02350 (OCL46_02350) pilJ 452906..453856 (+) 951 WP_003699627.1 PilW family protein Machinery gene
  OCL46_RS02355 (OCL46_02355) pilK 453835..454446 (+) 612 WP_033911077.1 PilX N-terminal domain-containing pilus assembly protein Machinery gene
  OCL46_RS02360 (OCL46_02360) pilL 454448..454921 (+) 474 WP_172760650.1 PilX family type IV pilin Machinery gene
  OCL46_RS02365 (OCL46_02365) - 454991..455299 (-) 309 WP_025456177.1 AzlD family protein -
  OCL46_RS02370 (OCL46_02370) - 455296..456006 (-) 711 Protein_465 AzlC family ABC transporter permease -
  OCL46_RS02375 (OCL46_02375) dut 456172..456624 (+) 453 WP_003699637.1 dUTP diphosphatase -
  OCL46_RS02380 (OCL46_02380) dapC 456700..457887 (+) 1188 WP_003699639.1 succinyldiaminopimelate transaminase -
  OCL46_RS02385 (OCL46_02385) yaaA 458043..458822 (+) 780 WP_003699641.1 peroxide stress protein YaaA -
  OCL46_RS02400 (OCL46_02400) - 459352..460545 (+) 1194 WP_017146746.1 phage integrase central domain-containing protein -
  OCL46_RS02405 (OCL46_02405) - 460901..461170 (-) 270 WP_003687928.1 hypothetical protein -
  OCL46_RS02410 (OCL46_02410) - 461365..462048 (-) 684 WP_003687929.1 DUF2786 domain-containing protein -
  OCL46_RS11770 - 462329..462595 (-) 267 Protein_472 hypothetical protein -
  OCL46_RS02420 (OCL46_02420) - 462706..462921 (-) 216 WP_003691538.1 hypothetical protein -
  OCL46_RS02425 (OCL46_02425) - 462973..463464 (-) 492 WP_033911206.1 siphovirus Gp157 family protein -
  OCL46_RS02430 (OCL46_02430) - 463461..463643 (-) 183 WP_003691535.1 hypothetical protein -
  OCL46_RS02435 (OCL46_02435) - 463783..464469 (-) 687 WP_010357532.1 phage replication initiation protein, NGO0469 family -
  OCL46_RS02440 (OCL46_02440) - 464538..464699 (-) 162 WP_003691530.1 hypothetical protein -
  OCL46_RS02445 (OCL46_02445) - 464696..464971 (-) 276 WP_033911205.1 NGO1622 family putative holin -
  OCL46_RS02450 (OCL46_02450) - 465124..465456 (-) 333 WP_003695500.1 hypothetical protein -
  OCL46_RS02455 (OCL46_02455) - 465598..465873 (-) 276 WP_229930546.1 hypothetical protein -
  OCL46_RS02460 (OCL46_02460) - 465870..466346 (-) 477 WP_002255718.1 DUF6948 domain-containing protein -
  OCL46_RS02465 (OCL46_02465) - 466379..466579 (-) 201 WP_003692842.1 hypothetical protein -
  OCL46_RS02470 (OCL46_02470) - 466777..467190 (-) 414 WP_003687963.1 hypothetical protein -
  OCL46_RS02475 (OCL46_02475) - 467187..467648 (-) 462 WP_003687965.1 helix-turn-helix domain-containing protein -
  OCL46_RS02480 (OCL46_02480) - 467665..468102 (-) 438 WP_003687967.1 hypothetical protein -
  OCL46_RS02485 (OCL46_02485) - 468215..468931 (-) 717 WP_003687969.1 LexA family transcriptional regulator -
  OCL46_RS02490 (OCL46_02490) - 469006..469236 (+) 231 WP_020997318.1 Cro/CI family transcriptional regulator -
  OCL46_RS02495 (OCL46_02495) - 469316..469471 (+) 156 WP_003698902.1 hypothetical protein -
  OCL46_RS02500 (OCL46_02500) - 469448..469636 (-) 189 WP_003691445.1 hypothetical protein -
  OCL46_RS02505 (OCL46_02505) - 469809..470036 (+) 228 WP_003698261.1 helix-turn-helix domain-containing protein -
  OCL46_RS02510 (OCL46_02510) - 470154..471218 (+) 1065 WP_003689134.1 hypothetical protein -
  OCL46_RS02515 (OCL46_02515) - 471215..472576 (+) 1362 WP_003706450.1 DnaB-like helicase C-terminal domain-containing protein -
  OCL46_RS02520 (OCL46_02520) - 472593..472844 (+) 252 WP_003703849.1 hypothetical protein -
  OCL46_RS02525 (OCL46_02525) - 472858..473352 (+) 495 WP_047923539.1 DUF3310 domain-containing protein -
  OCL46_RS02530 (OCL46_02530) - 473547..473696 (+) 150 WP_047920329.1 phage associated protein -
  OCL46_RS02535 (OCL46_02535) - 473725..474006 (+) 282 WP_003689109.1 hypothetical protein -
  OCL46_RS02540 (OCL46_02540) - 473997..474434 (+) 438 WP_229433529.1 RusA family crossover junction endodeoxyribonuclease -
  OCL46_RS02545 (OCL46_02545) - 474427..474732 (+) 306 WP_025456182.1 DUF1364 domain-containing protein -
  OCL46_RS02550 (OCL46_02550) - 474729..475112 (+) 384 WP_003699764.1 recombination protein NinB -
  OCL46_RS02555 (OCL46_02555) - 475103..475621 (+) 519 WP_003693459.1 HNH endonuclease -
  OCL46_RS02560 (OCL46_02560) - 475686..476108 (+) 423 WP_003690919.1 hypothetical protein -
  OCL46_RS02565 (OCL46_02565) - 476108..476647 (+) 540 WP_003693457.1 hypothetical protein -
  OCL46_RS02570 (OCL46_02570) - 476628..477902 (+) 1275 WP_003698265.1 PBSX family phage terminase large subunit -
  OCL46_RS02575 (OCL46_02575) - 477887..480154 (+) 2268 WP_229433533.1 hypothetical protein -
  OCL46_RS02580 (OCL46_02580) - 480392..481588 (+) 1197 WP_003693452.1 hypothetical protein -
  OCL46_RS02585 (OCL46_02585) - 481585..488892 (+) 7308 WP_003698268.1 PLxRFG domain-containing protein -
  OCL46_RS02590 (OCL46_02590) - 489518..490813 (+) 1296 WP_003693441.1 DUF4043 family protein -
  OCL46_RS02595 (OCL46_02595) - 490871..491344 (+) 474 WP_003693439.1 hypothetical protein -
  OCL46_RS02600 (OCL46_02600) - 491350..491835 (+) 486 WP_003693438.1 hypothetical protein -
  OCL46_RS02605 (OCL46_02605) - 491832..492506 (+) 675 WP_003693436.1 hypothetical protein -
  OCL46_RS02610 (OCL46_02610) - 492509..492658 (+) 150 WP_017146863.1 hypothetical protein -
  OCL46_RS02615 (OCL46_02615) - 492695..493546 (-) 852 WP_003699775.1 Bro-N domain-containing protein -

Sequence

Protein


Download         Length: 203 a.a.        Molecular weight: 22068.97 Da        Isoelectric Point: 7.5813

>NTDB_id=735568 OCL46_RS02355 WP_033911077.1 453835..454446(+) (pilK) [Neisseria gonorrhoeae strain 10531]
MRKQNALTGIPTSDGQRGFALFIVLMVMIVVAFLVVTAAQSYNTEQRISANESDRKLALSLAEAALREGEFQVLDLEYTA
DSKVTFSENCEKGLCTAVNVRTNNNGNEEAFGNIVVQGKPTVEAVKRSCPAKSGKNSTGLCIDNQGVEYEKGTASVSKMP
RYIIEYLGVKNGQNVYRVTAKAWGKNANTVVVLQSYVGNNDEQ

Nucleotide


Download         Length: 612 bp        

>NTDB_id=735568 OCL46_RS02355 WP_033911077.1 453835..454446(+) (pilK) [Neisseria gonorrhoeae strain 10531]
ATGCGCAAACAGAACGCTTTGACAGGAATCCCGACTTCTGACGGACAGAGGGGGTTCGCACTGTTTATCGTGCTGATGGT
GATGATAGTCGTGGCCTTTTTGGTTGTAACTGCCGCCCAGTCCTACAATACCGAACAGAGGATCAGTGCCAACGAATCAG
ACAGGAAATTGGCTTTGTCTTTAGCCGAGGCGGCTTTGAGGGAAGGCGAATTTCAGGTTTTGGATTTGGAATATACTGCG
GATAGTAAGGTTACATTTAGCGAAAACTGTGAAAAAGGCCTGTGTACCGCAGTGAATGTGCGGACAAATAATAATGGTAA
TGAAGAGGCTTTTGGCAATATCGTGGTGCAAGGCAAGCCTACCGTTGAGGCGGTGAAGCGTTCTTGCCCTGCAAAGTCTG
GCAAAAATTCTACCGGCCTGTGCATTGACAATCAGGGAGTGGAATATGAGAAAGGCACGGCAAGCGTCAGCAAAATGCCG
CGCTATATTATCGAATATTTAGGCGTGAAGAACGGACAAAATGTTTATCGGGTTACTGCCAAGGCTTGGGGTAAGAATGC
CAATACCGTGGTCGTCCTTCAATCTTATGTAGGCAATAATGATGAGCAATAA

Domains

Predicted by InterproScan.

PilX N-terminal (PilX_N)

(17-67)

Type IV pilus assembly protein PilX C-term (PilX)

(92-198)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilK Neisseria gonorrhoeae MS11

95.567

100

0.956