Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   P4B20_RS25440 Genome accession   NZ_CP120706
Coordinates   5395774..5396199 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain NY11104     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5390774..5401199
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P4B20_RS25425 (P4B20_25415) pilX 5391338..5391925 (+) 588 WP_003112826.1 type 4a pilus minor pilin PilX -
  P4B20_RS25430 (P4B20_25420) pilY1 5391937..5395428 (+) 3492 WP_010793785.1 type 4a pilus biogenesis protein PilY1 -
  P4B20_RS25435 (P4B20_25425) pilY2 5395430..5395777 (+) 348 WP_028681207.1 type 4a fimbrial biogenesis protein PilY2 -
  P4B20_RS25440 (P4B20_25430) comF 5395774..5396199 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  P4B20_RS25445 (P4B20_25435) ispH 5396246..5397190 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  P4B20_RS25450 (P4B20_25440) fkpB 5397276..5397716 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  P4B20_RS25455 (P4B20_25445) lspA 5397709..5398218 (-) 510 WP_003102615.1 signal peptidase II -
  P4B20_RS25460 (P4B20_25450) ileS 5398211..5401042 (-) 2832 WP_028681208.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=734222 P4B20_RS25440 WP_003094721.1 5395774..5396199(+) (comF) [Pseudomonas aeruginosa strain NY11104]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=734222 P4B20_RS25440 WP_003094721.1 5395774..5396199(+) (comF) [Pseudomonas aeruginosa strain NY11104]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383