Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   P4B08_RS25330 Genome accession   NZ_CP120704
Coordinates   5458795..5459220 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain SE5356     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5453795..5464220
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P4B08_RS25315 (P4B08_25325) pilX 5454359..5454946 (+) 588 WP_003112826.1 type 4a pilus minor pilin PilX -
  P4B08_RS25320 (P4B08_25330) pilY1 5454958..5458449 (+) 3492 WP_010793785.1 type 4a pilus biogenesis protein PilY1 -
  P4B08_RS25325 (P4B08_25335) pilY2 5458451..5458798 (+) 348 WP_028681207.1 type 4a fimbrial biogenesis protein PilY2 -
  P4B08_RS25330 (P4B08_25340) comF 5458795..5459220 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  P4B08_RS25335 (P4B08_25345) ispH 5459267..5460211 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  P4B08_RS25340 (P4B08_25350) fkpB 5460297..5460737 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  P4B08_RS25345 (P4B08_25355) lspA 5460730..5461239 (-) 510 WP_003102615.1 signal peptidase II -
  P4B08_RS25350 (P4B08_25360) ileS 5461232..5464063 (-) 2832 WP_028681208.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=734022 P4B08_RS25330 WP_003094721.1 5458795..5459220(+) (comF) [Pseudomonas aeruginosa strain SE5356]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=734022 P4B08_RS25330 WP_003094721.1 5458795..5459220(+) (comF) [Pseudomonas aeruginosa strain SE5356]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383