Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   N7921_RS13290 Genome accession   NZ_CP106652
Coordinates   2544908..2547232 (-) Length   774 a.a.
NCBI ID   WP_144484778.1    Uniprot ID   -
Organism   Bacillus safensis strain 33.4     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2539908..2552232
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N7921_RS13275 (N7921_13275) dtd 2541557..2542000 (-) 444 WP_095409179.1 D-aminoacyl-tRNA deacylase -
  N7921_RS13280 (N7921_13280) - 2542019..2544229 (-) 2211 WP_047203353.1 RelA/SpoT family protein -
  N7921_RS13285 (N7921_13285) - 2544372..2544884 (-) 513 WP_144484776.1 adenine phosphoribosyltransferase -
  N7921_RS13290 (N7921_13290) recJ 2544908..2547232 (-) 2325 WP_144484778.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  N7921_RS13295 (N7921_13295) - 2547314..2547664 (-) 351 WP_144484780.1 LapA family protein -
  N7921_RS13300 (N7921_13300) - 2547766..2548254 (-) 489 WP_041115109.1 cation:proton antiporter regulatory subunit -
  N7921_RS13305 (N7921_13305) secDF 2548440..2550668 (-) 2229 WP_144471246.1 protein translocase subunit SecDF -
  N7921_RS13310 (N7921_13310) - 2550710..2551006 (-) 297 WP_003216788.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 774 a.a.        Molecular weight: 87091.73 Da        Isoelectric Point: 5.0201

>NTDB_id=733728 N7921_RS13290 WP_144484778.1 2544908..2547232(-) (recJ) [Bacillus safensis strain 33.4]
MLLSKMRWEYEHPSEEKVKSLSENLNISALTASLLVKRGLEEVEEARSFLFDQKAEFHDPFLLKGMKEAVERINKAIEEQ
ESIVIFGDYDADGVTSTSVLLHTLKERSAIVDFYIPDRFKEGYGPNEQAFRYIKEQGASLVITVDTGIAAVNEARIAKEI
GLDLIITDHHEPSEELPEALAIVHPKQPGCEYPFKELAGVGVAFKVAHALLGKLPVQLLDLAAIGTIADLVPLHDENRWL
AKKGLVQLRQTNRPGLKALLKEAGATLEEANEETVGFQIAPRLNAVGRIEQADPAVHLLMTEDKDEAEELARFVQELNKE
RQKIVSTITEEAIQLVEEADDDQSAIVVAKAGWNPGVVGIVASKLTDTFSRPAIVLGIDEETQMAKGSARSIPGFDLFFH
LSKCRDILPHFGGHPMAAGMTLRADDVDLLRERLNQYANETLTKEDFIPIQRVDAVCKVDELTVEAIEEAGMLSPFGMSN
PKPFIMIEDAKLEDIRTIGANHNHIKMSLKDDDKLLDCVGFHQGKLKEEIVPGSRISVIGEMSVNEWNNRKKPQLMLKDA
RVDEWQLFDLRGKKDWEKKVSTLDSEKSLIVCFDEATKQDVDIPIHHVHAENVSSFNVHGKYVVFADVPADEQLLKQLLE
NQKPARIYTVFQRKEDHFMSSFPSRDQFKWFYGFLFKRGSFPIKEQGMELAKHRGWTKDTIIFMTKVFFELGFVKIENGV
LSIVLDAPKKDLTASASYTAKQRLMELDQTLTYSSAKELKEWLNSMMSEVSPVL

Nucleotide


Download         Length: 2325 bp        

>NTDB_id=733728 N7921_RS13290 WP_144484778.1 2544908..2547232(-) (recJ) [Bacillus safensis strain 33.4]
ATGTTATTATCCAAAATGCGCTGGGAATATGAACATCCAAGCGAAGAAAAAGTGAAATCACTCTCTGAAAATCTGAATAT
TTCAGCGCTGACTGCATCGCTTCTCGTAAAAAGAGGACTTGAAGAAGTAGAAGAGGCGAGGTCTTTTTTATTTGATCAAA
AAGCCGAATTTCATGATCCATTCCTATTAAAAGGAATGAAAGAAGCAGTAGAGCGCATCAACAAAGCGATCGAAGAACAG
GAATCGATTGTGATCTTCGGAGACTATGATGCAGATGGTGTGACAAGTACGTCTGTACTTTTACATACATTAAAAGAGCG
GTCTGCGATAGTAGACTTTTATATTCCAGATCGATTTAAAGAAGGCTATGGGCCTAATGAACAGGCATTTCGATATATCA
AGGAGCAAGGAGCATCGCTTGTCATCACGGTCGATACAGGCATTGCTGCTGTCAATGAAGCACGTATTGCAAAAGAAATA
GGGTTAGACCTAATCATTACAGATCACCATGAACCAAGTGAGGAGCTGCCTGAGGCACTTGCCATTGTCCATCCTAAGCA
GCCGGGCTGTGAGTATCCCTTTAAAGAACTTGCTGGTGTAGGTGTCGCGTTTAAAGTGGCGCATGCACTTCTTGGGAAGC
TGCCAGTTCAGCTGTTAGATCTTGCAGCAATCGGGACAATCGCGGATCTTGTACCGCTTCATGATGAGAATAGGTGGCTC
GCCAAAAAAGGTCTTGTGCAGCTTCGACAGACGAATAGACCGGGGCTGAAAGCGTTATTGAAAGAAGCGGGAGCCACTCT
AGAAGAAGCCAATGAGGAAACGGTCGGCTTTCAAATTGCGCCAAGGCTGAATGCTGTTGGGCGAATTGAACAGGCAGATC
CAGCCGTTCATCTTCTTATGACTGAGGATAAGGATGAAGCAGAAGAGCTCGCTCGTTTTGTACAGGAACTGAATAAAGAA
CGACAAAAGATTGTCAGTACGATCACAGAAGAAGCGATTCAATTGGTGGAAGAGGCAGATGATGACCAGTCTGCGATCGT
TGTAGCGAAAGCTGGATGGAATCCGGGCGTTGTCGGAATTGTTGCCTCTAAATTGACGGATACTTTCTCTCGTCCAGCGA
TTGTACTTGGAATAGATGAAGAGACGCAAATGGCAAAAGGATCAGCCCGGAGTATTCCTGGCTTCGATCTATTTTTCCAT
CTAAGCAAATGCCGCGACATCCTGCCGCATTTCGGCGGGCATCCGATGGCAGCAGGGATGACACTTCGAGCGGATGATGT
CGATTTACTAAGGGAAAGATTAAATCAATATGCAAATGAAACATTGACGAAAGAAGATTTTATTCCAATCCAGCGAGTCG
ATGCAGTGTGTAAGGTAGACGAATTGACGGTTGAAGCGATTGAAGAAGCGGGGATGCTGTCGCCATTCGGTATGTCAAAC
CCAAAACCGTTCATTATGATTGAAGATGCGAAGCTTGAAGATATCCGAACGATTGGGGCAAATCACAACCATATTAAAAT
GTCTCTGAAAGACGATGACAAGCTGCTTGATTGTGTCGGTTTTCACCAAGGGAAATTAAAAGAAGAAATTGTTCCAGGAA
GCCGCATTTCCGTGATTGGTGAAATGTCCGTCAATGAATGGAATAACCGGAAGAAGCCCCAGCTGATGCTAAAAGATGCA
CGAGTTGATGAATGGCAGCTGTTTGATCTGCGTGGGAAAAAGGATTGGGAGAAAAAAGTTTCGACGCTTGATTCAGAAAA
ATCTTTGATTGTCTGTTTTGATGAAGCAACAAAACAGGACGTCGATATCCCTATTCATCATGTTCATGCAGAAAATGTAT
CATCGTTTAATGTACACGGAAAATATGTGGTCTTTGCGGATGTTCCCGCAGATGAGCAATTGCTGAAGCAGCTTTTAGAA
AACCAAAAGCCTGCCAGAATATATACGGTTTTTCAAAGAAAAGAAGATCATTTTATGTCCTCTTTCCCAAGCCGTGACCA
ATTCAAATGGTTTTATGGGTTCTTGTTTAAACGGGGAAGTTTTCCTATCAAAGAACAAGGAATGGAGCTTGCCAAACATA
GAGGCTGGACAAAAGATACAATCATTTTTATGACAAAGGTGTTTTTTGAACTCGGTTTTGTTAAAATAGAGAATGGTGTG
CTGTCAATAGTACTTGATGCCCCTAAAAAAGATTTAACGGCATCAGCGTCCTATACAGCAAAACAACGATTGATGGAACT
CGATCAAACACTAACATATTCCTCAGCTAAAGAGCTGAAGGAATGGCTGAATAGCATGATGAGCGAAGTCTCACCTGTGC
TTTGA

Domains

Predicted by InterproScan.

DHHA1 domain (DHHA1)

(312-437)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-763)

RecJ OB domain (RecJ_OB)

(453-559)

DHH family (DHH)

(83-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

67.181

100

0.674