Detailed information NTG0727921

Bioinformatically predicted

Overview

• Name: amiF
• Type: Regulator
• Locus tag: N2A95_RS01275
• Genome accession: NZ_CP104190
• Coordinates: 253124..254059 (+)
• Length: 311 a.a.
• NCBI ID: WP_003105521.1
• Uniprot ID: A0A2I8AI54
• Organism: Streptococcus parauberis strain HFTC0064
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 248124..259059

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N2A95_RS01255 (N2A95_01255)	-	248377..250035 (+)	1659	WP_003107719.1	peptide ABC transporter substrate-binding protein	-
N2A95_RS01260 (N2A95_01260)	-	250111..251025 (+)	915	WP_004347295.1	ABC transporter permease	-
N2A95_RS01265 (N2A95_01265)	-	251035..252066 (+)	1032	WP_004347296.1	ABC transporter permease	-
N2A95_RS01270 (N2A95_01270)	oppD	252081..253124 (+)	1044	WP_003103717.1	ABC transporter ATP-binding protein	Regulator
N2A95_RS01275 (N2A95_01275)	amiF	253124..254059 (+)	936	WP_003105521.1	ABC transporter ATP-binding protein	Regulator
N2A95_RS01280 (N2A95_01280)	rnjA	254125..255807 (-)	1683	WP_003107714.1	ribonuclease J1	-
N2A95_RS01285 (N2A95_01285)	-	255809..256039 (-)	231	WP_003102975.1	DNA-dependent RNA polymerase subunit epsilon	-
N2A95_RS01290 (N2A95_01290)	tsaB	256312..257004 (+)	693	WP_037620071.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB	-
N2A95_RS01295 (N2A95_01295)	rimI	257009..257425 (+)	417	WP_003105855.1	ribosomal protein S18-alanine N-acetyltransferase	-
N2A95_RS01300 (N2A95_01300)	tsaD	257415..258428 (+)	1014	WP_003105067.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD	-

Sequence

Protein

• Length: 311 a.a.
• Molecular weight: 35205.16 Da
• Isoelectric Point: 6.5131

>NTDB_id=727921 N2A95_RS01275 WP_003105521.1 253124..254059(+) (amiF) [Streptococcus parauberis strain HFTC0064]
MTEERKKLVELKNVSLTFNKGKSNEVKAINNVSFDIYEGEVFGLVGESGSGKTTVGRAILKLYEITDGEIIFNGVNITKL
KGKELHNFRKDAQMIFQDPQASLNGRMKIRDIVAEGLDIHGLTKTKEEREARVQELLDRVGLNKDHVTRYPHEFSGGQRQ
RIGIARALAVKPKFIIADEPISALDVSIQAQVVNLMQKLQTERGLTYLFIAHDLSMVKYISDRIGVMHWGKMLEIGTSED
VYNNPIHPYTRSLLSAIPEPDPDYERQRVAEIYDPSIELDGKEREMREITPGHFVFSTEEEAVAYKANSNK

Nucleotide

• Length: 936 bp

>NTDB_id=727921 N2A95_RS01275 WP_003105521.1 253124..254059(+) (amiF) [Streptococcus parauberis strain HFTC0064]
ATGACTGAAGAACGTAAAAAATTAGTTGAACTAAAAAACGTTTCTTTAACCTTTAATAAAGGGAAAAGCAACGAAGTCAA
GGCCATTAATAATGTCAGTTTTGATATCTATGAAGGTGAAGTCTTTGGATTAGTTGGGGAATCAGGTTCTGGTAAAACAA
CAGTTGGCCGTGCTATCTTAAAACTTTATGAAATTACCGATGGCGAAATTATTTTCAATGGTGTAAATATCACTAAACTA
AAAGGAAAAGAACTTCATAATTTCCGTAAAGATGCACAAATGATTTTCCAAGATCCACAAGCAAGTCTTAATGGACGGAT
GAAAATTAGAGACATCGTTGCTGAAGGACTAGATATTCATGGTTTAACTAAAACAAAGGAAGAACGTGAAGCTCGTGTTC
AAGAACTACTTGACCGTGTTGGTTTGAATAAAGACCATGTGACTCGTTATCCACATGAATTTTCAGGAGGACAACGTCAA
CGTATTGGTATTGCCCGTGCCTTGGCTGTAAAACCCAAATTTATCATTGCTGATGAACCAATTTCTGCTTTGGACGTATC
TATCCAAGCACAGGTTGTTAACTTGATGCAAAAATTACAAACTGAACGTGGTTTAACCTACTTGTTTATCGCCCATGATT
TATCAATGGTTAAATACATTTCAGATCGTATTGGTGTTATGCACTGGGGTAAAATGTTAGAAATTGGGACATCTGAAGAT
GTTTATAATAACCCAATTCACCCATATACAAGAAGTCTCTTATCAGCTATTCCTGAACCAGACCCAGATTATGAACGCCA
ACGTGTTGCGGAAATTTATGATCCAAGTATTGAATTGGATGGAAAAGAACGTGAAATGCGTGAAATCACACCAGGTCACT
TCGTATTTTCAACAGAAGAAGAAGCAGTAGCTTACAAAGCAAATTCAAATAAATAA

Domains

Predicted by InterproScan.

• ABC transporter (ABC_tran) (30-181)

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-265)

3D structure

• AlphaFold DB: A0A2I8AI54

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiF	Streptococcus thermophilus LMD-9	54.221	99.035	0.537
amiF	Streptococcus thermophilus LMG 18311	53.896	99.035	0.534
amiF	Streptococcus salivarius strain HSISS4	53.247	99.035	0.527