Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   PUN20_RS24825 Genome accession   NZ_CP118638
Coordinates   5311538..5311963 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain P9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5306538..5316963
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUN20_RS24810 (PUN20_24795) pilX 5307102..5307689 (+) 588 WP_003112826.1 type 4a pilus minor pilin PilX -
  PUN20_RS24815 (PUN20_24800) pilY1 5307701..5311192 (+) 3492 WP_003123397.1 type 4a pilus biogenesis protein PilY1 -
  PUN20_RS24820 (PUN20_24805) pilY2 5311194..5311541 (+) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  PUN20_RS24825 (PUN20_24810) comF 5311538..5311963 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  PUN20_RS24830 (PUN20_24815) ispH 5312010..5312954 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  PUN20_RS24835 (PUN20_24820) fkpB 5313040..5313480 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  PUN20_RS24840 (PUN20_24825) lspA 5313473..5313982 (-) 510 WP_003102615.1 signal peptidase II -
  PUN20_RS24845 (PUN20_24830) ileS 5313975..5316806 (-) 2832 WP_014603309.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=723223 PUN20_RS24825 WP_003094721.1 5311538..5311963(+) (comF) [Pseudomonas aeruginosa strain P9]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=723223 PUN20_RS24825 WP_003094721.1 5311538..5311963(+) (comF) [Pseudomonas aeruginosa strain P9]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCGCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383