Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   EKM74_RS01085 Genome accession   NZ_AP019306
Coordinates   215542..216612 (-) Length   356 a.a.
NCBI ID   WP_000776422.1    Uniprot ID   -
Organism   Staphylococcus aureus strain TUM9463     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 210542..221612
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EKM74_RS01045 gcvPA 210719..212065 (-) 1347 WP_000019691.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  EKM74_RS01050 gcvT 212085..213176 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  EKM74_RS01055 - 213335..213859 (-) 525 WP_001015120.1 shikimate kinase -
  EKM74_RS01060 - 213849..213995 (-) 147 WP_001792109.1 hypothetical protein -
  EKM74_RS01065 comGF 214092..214589 (-) 498 WP_001788897.1 competence type IV pilus minor pilin ComGF Machinery gene
  EKM74_RS01070 comGE 214507..214806 (-) 300 WP_000844410.1 hypothetical protein Machinery gene
  EKM74_RS01075 comGD 214793..215239 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  EKM74_RS01080 comGC 215217..215528 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  EKM74_RS01085 comGB 215542..216612 (-) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  EKM74_RS01090 comGA 216584..217558 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  EKM74_RS01095 - 217610..218233 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  EKM74_RS01100 - 218230..218559 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  EKM74_RS01105 - 218559..219545 (-) 987 WP_000161311.1 ROK family glucokinase -
  EKM74_RS01110 - 219542..219745 (-) 204 WP_000087561.1 YqgQ family protein -
  EKM74_RS01115 - 219726..221189 (-) 1464 WP_042109137.1 rhomboid family protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 41447.84 Da        Isoelectric Point: 10.0606

>NTDB_id=72289 EKM74_RS01085 WP_000776422.1 215542..216612(-) (comGB) [Staphylococcus aureus strain TUM9463]
MKLQWINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSFQFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIG
YSDTIVMQVYLAERFGNIIDVLEETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNNLNMLNKINFVMKLPLISGYFQLFKTYFVTNELVLFYKNGITLQ
SIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQILEKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIK
QTQFLQPILFLILGLFIVAIYLVIMLPMFQMMQSIK

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=72289 EKM74_RS01085 WP_000776422.1 215542..216612(-) (comGB) [Staphylococcus aureus strain TUM9463]
GTGAAACTACAATGGATAAATACATTTAAACTACATTCTAAGAAGCGACAATTAAGTAAGGCCCAACAAATCGATTTACT
TTCAAATTTATGTAATTTGTTGAAATATGGTTTCACTCTGTATCAAAGTTTTCAATTTTTAAATCTTCAAATGACATATA
AAAATAAGCAATTAGGTACCACCATTCTAAGTGAAATTTCAAATGGTGCACCATGCAATCAAATATTATCACTGATAGGT
TATAGCGATACTATCGTCATGCAAGTATATTTGGCAGAAAGATTTGGCAATATTATAGACGTTCTAGAAGAAACCGTAAA
TTATATGAAAGTGAATAGAAAGTCAGAACAACGATTGTTAAAGACACTGCAATACCCCTTAATACTAGTTTCTATCTTTA
TTGCTATGATTATTATATTAAACCTCACAGTAATTCCACAGTTTCAACAATTATATACTTCTATGAATATTCAACTATCT
TCTTTTCAAAAAACATTGTCTTTTTTCATTACCAGCTTACCTACTATAATTGTAGTAATGCTCATAATAGTATCTATGTT
GGCTATTATTATGAAATTAATTTATAACAATTTAAATATGCTCAATAAGATAAACTTTGTGATGAAACTACCGCTAATAT
CAGGCTATTTCCAATTATTTAAAACTTATTTTGTAACTAATGAATTAGTGTTGTTTTATAAAAATGGTATTACACTTCAA
TCAATAGTAGACGTTTATATTAACCATAGTAGTGATCCATTTAGACAGTTTCTAGGTAAATACTTATTAACTTATTCAGA
AATGGGATATGGTTTGCCTCAAATTTTAGAAAAACTAAAATGCTTTAAGCCTCAATTAATTAAGTTTGTGCTACAAGGTG
AAAAGAGAGGGAAGCTAGAAGTAGAACTAAAGTTATATTCGCAAATATTAGTAAAACAAATAGAAGATAAAGCGATAAAA
CAGACTCAGTTTTTACAGCCTATTTTATTTTTGATTTTAGGTTTATTTATTGTCGCAATTTATTTAGTAATTATGTTACC
AATGTTTCAAATGATGCAAAGTATAAAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(227-347)

Type II secretion system (T2SS), protein F (T2SSF)

(27-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Staphylococcus aureus MW2

100

100

1
  comGB Staphylococcus aureus N315

100

100

1

Multiple sequence alignment