Detailed information NTG0722791

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: NYO12_RS23315
• Genome accession: NZ_CP103420
• Coordinates: 4673293..4674093 (+)
• Length: 266 a.a.
• NCBI ID: WP_008807384.1
• Uniprot ID: A0A0J4LMF2
• Organism: Klebsiella variicola strain KV1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4668293..4679093

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NYO12_RS23280 (NYO12_23280)	gspH	4668449..4668964 (+)	516	WP_038422126.1	type II secretion system minor pseudopilin GspH	-
NYO12_RS23285 (NYO12_23285)	gspI	4668961..4669326 (+)	366	WP_004894160.1	type II secretion system minor pseudopilin GspI	-
NYO12_RS23290 (NYO12_23290)	gspJ	4669323..4669919 (+)	597	WP_008807379.1	type II secretion system minor pseudopilin GspJ	-
NYO12_RS23295 (NYO12_23295)	gspK	4669916..4670896 (+)	981	WP_012542824.1	type II secretion system minor pseudopilin GspK	-
NYO12_RS23300 (NYO12_23300)	gspL	4670889..4672082 (+)	1194	WP_259072199.1	type II secretion system protein GspL	-
NYO12_RS23305 (NYO12_23305)	-	4672075..4672560 (+)	486	WP_259072201.1	type II secretion system protein M	-
NYO12_RS23310 (NYO12_23310)	-	4672550..4673296 (+)	747	WP_259072202.1	type II secretion system protein N	-
NYO12_RS23315 (NYO12_23315)	pilD	4673293..4674093 (+)	801	WP_008807384.1	A24 family peptidase	Machinery gene
NYO12_RS23320 (NYO12_23320)	hrpB	4674140..4676569 (-)	2430	WP_259072208.1	ATP-dependent helicase HrpB	-
NYO12_RS23325 (NYO12_23325)	thpR	4676642..4677178 (+)	537	WP_008807387.1	RNA 2',3'-cyclic phosphodiesterase	-
NYO12_RS23330 (NYO12_23330)	sfsA	4677178..4677894 (+)	717	WP_008807388.1	DNA/RNA nuclease SfsA	-
NYO12_RS23335 (NYO12_23335)	dksA	4678057..4678512 (+)	456	WP_007749299.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29433.14 Da
• Isoelectric Point: 8.7151

>NTDB_id=722791 NYO12_RS23315 WP_008807384.1 4673293..4674093(+) (pilD) [Klebsiella variicola strain KV1]
MTTLTALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLLLLSFLLMLARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASACGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=722791 NYO12_RS23315 WP_008807384.1 4673293..4674093(+) (pilD) [Klebsiella variicola strain KV1]
ATGACGACATTAACGGCATTATCGCTGCACTTTCCCCTTGTCTGGTATGGCTTCCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCCTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGCTGCTGCTCTCGTTCCTGCTGATGCTGGCGC
GGATTGACGCCCGGACCCAGCTGTTGCCCGACCGTCTGACGCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGACGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCCTCGGCCTGCGGGCTGGTGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGAGGGGGCATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

3D structure

• AlphaFold DB: A0A0J4LMF2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.455	100	0.47
pilD	Acinetobacter nosocomialis M2	45.627	98.872	0.451
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425