Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   PT746_RS31785 Genome accession   NZ_CP117974
Coordinates   6725926..6726351 (-) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain B-3509     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6720926..6731351
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PT746_RS31765 ileS 6721083..6723914 (+) 2832 WP_004352756.1 isoleucine--tRNA ligase -
  PT746_RS31770 lspA 6723907..6724416 (+) 510 WP_003102615.1 signal peptidase II -
  PT746_RS31775 fkpB 6724409..6724849 (+) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  PT746_RS31780 ispH 6724935..6725879 (+) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  PT746_RS31785 comF 6725926..6726351 (-) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  PT746_RS31790 pilY2 6726348..6726695 (-) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  PT746_RS31795 pilY1 6726697..6730188 (-) 3492 WP_003123397.1 type 4a pilus biogenesis protein PilY1 -
  PT746_RS31800 pilX 6730200..6730787 (-) 588 WP_003112826.1 type 4a pilus minor pilin PilX -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=720056 PT746_RS31785 WP_003094721.1 6725926..6726351(-) (comF) [Pseudomonas aeruginosa strain B-3509]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=720056 PT746_RS31785 WP_003094721.1 6725926..6726351(-) (comF) [Pseudomonas aeruginosa strain B-3509]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCGCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383