Detailed information    

experimental Experimentally validated

Overview

Name   F7686_RS05975   Type   Unclear
Locus tag   F7686_RS05975 Genome accession   NZ_FNFT01000004
Coordinates   169520..170731 (+) Length   403 a.a.
NCBI ID   WP_066957359.1    Uniprot ID   A0A1G8ZHJ9
Organism   Methanoculleus thermophilus strain DSM 2373     
Function   require for natural transformation   
Unclear

Function

The ΔSAMN04488571_104187 mutant strain of M. thermophilus was completely defective for natural transformation.


Genomic Context

Location: 164520..175731
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F7686_RS05950 (SAMN04488571_104182) - 165149..166330 (+) 1182 WP_066957367.1 MFS transporter -
  F7686_RS05955 (SAMN04488571_104183) - 166385..167533 (+) 1149 WP_066957365.1 CPBP family intramembrane glutamic endopeptidase -
  F7686_RS05965 (SAMN04488571_104185) - 167836..168744 (-) 909 WP_066957363.1 RNA-guided pseudouridylation complex pseudouridine synthase subunit Cbf5 -
  F7686_RS05970 (SAMN04488571_104186) - 168750..169430 (-) 681 WP_066957361.1 uroporphyrinogen-III synthase -
  F7686_RS05975 (SAMN04488571_104187) F7686_RS05975 169520..170731 (+) 1212 WP_066957359.1 tripartite tricarboxylate transporter permease Unclear
  F7686_RS05980 (SAMN04488571_104188) - 170766..171626 (+) 861 WP_066957358.1 NOP5/NOP56 family protein -
  F7686_RS05985 (SAMN04488571_104189) - 171623..172225 (+) 603 WP_066957355.1 fibrillarin-like rRNA/tRNA 2'-O-methyltransferase -
  F7686_RS05990 (SAMN04488571_104190) - 172259..172993 (+) 735 WP_066957353.1 DUF1614 domain-containing protein -
  F7686_RS05995 (SAMN04488571_104191) - 173020..173859 (-) 840 WP_066957351.1 TIM barrel protein -
  F7686_RS06005 (SAMN04488571_104193) - 174025..174756 (-) 732 WP_066957349.1 thymidylate synthase -

Sequence

Protein


Download         Length: 403 a.a.        Molecular weight: 40998.65 Da        Isoelectric Point: 6.3183

>NTDB_id=72 F7686_RS05975 WP_066957359.1 169520..170731(+) (F7686_RS05975) [Methanoculleus thermophilus strain DSM 2373]
MLISILLGAAVGIGCGAVSGLVPGIHANTVAGLLLSLQALLLAWFDPVFIASAMFATLVAHTFLDNVPGTFLGIPDADTS
LAVLPAHALCLDGRGEEAVRISALGSALGVVLSLPLALAFVLVLPALQPAIDWGIGLVILAVAGYLIVVSESPGWAFAVF
SVSGVLGLFSLGYSFLAWSTGGESGVLMPLLSGLFGIAVLLQASHGAMPVQHFSGIDLPPGALRRGSLLGSAVGALVGWL
PGLSNATANALLTSVVGYDTNPREYILATSAANTVNAFLGLAAFYAISRTRSGVMAAIGALEEIPPATAILLAGAIAAVG
AYLLTVRLSSMAAWFSGMSVKKLNYGVIAFVALLSFFLCGPFGVLVLILATAVGYVPALVNIRRVYCMGAIMVPVMAYSF
GLA

Nucleotide


Download         Length: 1212 bp        

>NTDB_id=72 F7686_RS05975 WP_066957359.1 169520..170731(+) (F7686_RS05975) [Methanoculleus thermophilus strain DSM 2373]
GTGCTCATAAGTATTCTGCTCGGGGCCGCGGTCGGGATCGGTTGCGGCGCCGTGAGCGGTCTTGTACCCGGGATCCATGC
AAACACCGTGGCGGGTCTTCTCCTATCGCTCCAGGCGCTGCTGCTCGCCTGGTTCGACCCGGTGTTCATCGCCTCTGCGA
TGTTTGCCACGCTTGTAGCGCATACGTTCCTCGACAACGTCCCGGGCACCTTCCTTGGCATCCCGGATGCCGACACCTCG
CTTGCGGTCCTCCCGGCGCACGCCCTCTGCCTCGATGGTCGGGGTGAGGAGGCCGTCCGGATATCGGCTCTCGGGAGCGC
TCTTGGTGTCGTCCTCTCCCTCCCGCTCGCGCTCGCCTTCGTCCTCGTCCTCCCGGCGCTCCAGCCGGCGATCGACTGGG
GGATTGGTCTTGTCATCCTCGCGGTTGCGGGCTACCTCATCGTCGTCTCCGAATCTCCCGGCTGGGCGTTCGCGGTCTTT
TCGGTCTCCGGGGTTCTCGGACTCTTCTCGCTCGGCTACTCATTCCTCGCCTGGTCGACGGGCGGGGAGTCCGGGGTGCT
GATGCCCCTCCTCTCGGGGCTTTTCGGGATCGCGGTCCTCCTCCAGGCATCGCACGGGGCGATGCCGGTGCAGCACTTCT
CGGGGATCGATCTCCCGCCGGGTGCGCTCCGGCGGGGCTCTCTCCTCGGCTCGGCTGTCGGAGCCCTCGTCGGCTGGCTC
CCGGGCCTCTCGAACGCCACGGCTAACGCCCTTCTCACCTCGGTCGTCGGCTACGACACAAACCCCCGGGAGTACATCCT
CGCGACCAGCGCCGCAAACACCGTGAACGCTTTCCTCGGGCTTGCCGCCTTCTACGCCATATCCCGGACGCGGAGCGGCG
TGATGGCCGCGATCGGGGCGCTCGAAGAGATCCCGCCTGCGACCGCGATCCTCCTTGCGGGTGCGATAGCCGCAGTCGGG
GCCTACCTCCTGACGGTCCGCCTCTCCTCGATGGCCGCGTGGTTCTCCGGCATGAGCGTCAAAAAGTTAAATTACGGCGT
CATCGCCTTTGTCGCCCTCCTTTCCTTCTTCCTCTGCGGCCCGTTCGGGGTGCTCGTCCTCATACTTGCTACGGCCGTCG
GCTACGTCCCGGCCCTCGTCAACATCCGCCGGGTCTACTGCATGGGGGCGATCATGGTCCCGGTGATGGCCTACTCATTC
GGGCTCGCCTGA

Domains

Predicted by InterproScan.

Tripartite tricarboxylate transporter TctA family (TctA)

(6-388)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1G8ZHJ9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Dallas R Fonseca et al. (2023) Random transposon mutagenesis identifies genes essential for transformation in Methanococcus maripaludis. Molecular Genetics And Genomics : MGG 298(3):537-548. [PMID: 36823423]