Detailed information NTG0714987

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: NQZ90_RS00735
• Genome accession: NZ_CP102148
• Coordinates: 118585..119019 (+)
• Length: 144 a.a.
• NCBI ID: WP_141599726.1
• Uniprot ID: A0A540U4B8
• Organism: Streptococcus suis strain DNC15
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 113585..124019

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NQZ90_RS00700 (NQZ90_00700)	-	114327..114692 (+)	366	WP_014637310.1	DUF1033 family protein	-
NQZ90_RS00705 (NQZ90_00705)	-	114727..115677 (-)	951	WP_014637311.1	S66 peptidase family protein	-
NQZ90_RS00710 (NQZ90_00710)	comYA	115763..116713 (+)	951	WP_203200325.1	competence type IV pilus ATPase ComGA	Machinery gene
NQZ90_RS00715 (NQZ90_00715)	comYB	116625..117662 (+)	1038	WP_257110428.1	competence type IV pilus assembly protein ComGB	Machinery gene
NQZ90_RS00720 (NQZ90_00720)	comYC	117664..117945 (+)	282	WP_141599722.1	competence type IV pilus major pilin ComGC	Machinery gene
NQZ90_RS00725 (NQZ90_00725)	comGD	117926..118333 (+)	408	WP_141599724.1	competence type IV pilus minor pilin ComGD	-
NQZ90_RS00730 (NQZ90_00730)	comYE	118305..118598 (+)	294	WP_024405248.1	competence type IV pilus minor pilin ComGE	Machinery gene
NQZ90_RS00735 (NQZ90_00735)	comGF/cglF	118585..119019 (+)	435	WP_141599726.1	competence type IV pilus minor pilin ComGF	Machinery gene
NQZ90_RS00740 (NQZ90_00740)	comGG	118997..119407 (+)	411	WP_024415503.1	competence type IV pilus minor pilin ComGG	-
NQZ90_RS00745 (NQZ90_00745)	comYH	119464..120417 (+)	954	WP_141599728.1	class I SAM-dependent methyltransferase	Machinery gene
NQZ90_RS00750 (NQZ90_00750)	-	120467..121654 (+)	1188	WP_013730078.1	acetate kinase	-
NQZ90_RS00755 (NQZ90_00755)	-	121968..122519 (+)	552	WP_257110272.1	folate family ECF transporter S component	-
NQZ90_RS00760 (NQZ90_00760)	-	122574..123830 (+)	1257	WP_257110273.1	folylpolyglutamate synthase/dihydrofolate synthase family protein	-

Sequence

Protein

• Length: 144 a.a.
• Molecular weight: 16405.76 Da
• Isoelectric Point: 7.9738

>NTDB_id=714987 NQZ90_RS00735 WP_141599726.1 118585..119019(+) (comGF/cglF) [Streptococcus suis strain DNC15]
MLKSKIPAFTLLECLVAVVILSGSLLVFEGLSKLLVQEAHYQGQTVQKEWLVFSSQLRSEWDRAELVKVENGKVYVNKGE
QALAFGKSRSDDFRKTNDKGQGYQPMIYQVDSAAISQENQLVRIDFSFKNGEERTFIYAFKEKS

Nucleotide

• Length: 435 bp

>NTDB_id=714987 NQZ90_RS00735 WP_141599726.1 118585..119019(+) (comGF/cglF) [Streptococcus suis strain DNC15]
TTGTTAAAAAGTAAAATTCCTGCTTTTACTCTCTTGGAATGCTTGGTAGCCGTAGTCATCCTTTCAGGTAGTCTTCTGGT
CTTTGAAGGCTTGTCAAAACTGCTGGTTCAAGAAGCGCATTACCAAGGTCAGACCGTTCAAAAGGAATGGTTGGTCTTCT
CTAGTCAATTGCGGTCGGAGTGGGACCGGGCTGAATTGGTCAAGGTTGAAAATGGCAAGGTCTATGTCAACAAAGGCGAA
CAGGCTTTGGCCTTTGGCAAGTCACGGTCGGATGATTTTCGGAAAACTAATGATAAGGGACAGGGCTATCAACCTATGAT
TTATCAGGTGGACAGCGCAGCTATTTCCCAAGAAAACCAGCTGGTGCGCATCGACTTTAGCTTTAAAAACGGAGAGGAGC
GGACCTTTATCTATGCTTTTAAAGAAAAAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (37-134)

3D structure

• AlphaFold DB: A0A540U4B8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus pneumoniae TIGR4	52.482	97.917	0.514
comGF/cglF	Streptococcus pneumoniae Rx1	52.482	97.917	0.514
comGF/cglF	Streptococcus pneumoniae D39	52.482	97.917	0.514
comGF/cglF	Streptococcus pneumoniae R6	52.482	97.917	0.514
comGF/cglF	Streptococcus mitis NCTC 12261	53.731	93.056	0.5
comYF	Streptococcus mutans UA140	48.611	100	0.486
comYF	Streptococcus mutans UA159	47.917	100	0.479
comGF/cglF	Streptococcus mitis SK321	50.746	93.056	0.472
comGF	Lactococcus lactis subsp. cremoris KW2	46.099	97.917	0.451