Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   PP478_RS26505 Genome accession   NZ_CP116933
Coordinates   5695536..5695961 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain CUVET21-397     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5690536..5700961
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PP478_RS26490 (PP478_26470) pilX 5691100..5691687 (+) 588 WP_003094700.1 type 4a pilus minor pilin PilX -
  PP478_RS26495 (PP478_26475) pilY1 5691699..5695190 (+) 3492 WP_003094704.1 type 4a pilus biogenesis protein PilY1 -
  PP478_RS26500 (PP478_26480) pilY2 5695192..5695539 (+) 348 WP_003094713.1 type 4a fimbrial biogenesis protein PilY2 -
  PP478_RS26505 (PP478_26485) comF 5695536..5695961 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  PP478_RS26510 (PP478_26490) ispH 5696008..5696952 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  PP478_RS26515 (PP478_26495) fkpB 5697038..5697478 (-) 441 WP_003094726.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  PP478_RS26520 (PP478_26500) lspA 5697471..5697980 (-) 510 WP_003094728.1 signal peptidase II -
  PP478_RS26525 (PP478_26505) ileS 5697973..5700804 (-) 2832 WP_003094730.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=714655 PP478_RS26505 WP_003094721.1 5695536..5695961(+) (comF) [Pseudomonas aeruginosa strain CUVET21-397]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=714655 PP478_RS26505 WP_003094721.1 5695536..5695961(+) (comF) [Pseudomonas aeruginosa strain CUVET21-397]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGCTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCGCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383