Detailed information NTG0713217

Bioinformatically predicted

Overview

• Name: addB
• Type: Machinery gene
• Locus tag: NP434_RS05770
• Genome accession: NZ_CP101936
• Coordinates: 1106670..1110170 (+)
• Length: 1166 a.a.
• NCBI ID: WP_256683338.1
• Uniprot ID: -
• Organism: Bacillus subtilis subsp. subtilis strain BGSC 10A5
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1101670..1115170

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NP434_RS05750 (NP434_05750)	yhjO	1102529..1103734 (-)	1206	WP_256683336.1	MFS transporter	-
NP434_RS05755 (NP434_05755)	yhjP	1103848..1105575 (+)	1728	WP_256683337.1	SgrR family transcriptional regulator	-
NP434_RS05760 (NP434_05760)	cusC	1105605..1105931 (-)	327	WP_033880479.1	four-helix bundle copper-binding protein	-
NP434_RS05765 (NP434_05765)	yhjR	1106049..1106486 (-)	438	WP_029317584.1	ferritin family protein	-
NP434_RS05770 (NP434_05770)	addB	1106670..1110170 (+)	3501	WP_256683338.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
NP434_RS05775 (NP434_05775)	addA	1110157..1113855 (+)	3699	WP_256683339.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
NP434_RS05780 (NP434_05780)	sbcD	1113927..1115102 (+)	1176	WP_033883628.1	exonuclease subunit SbcD	-

Sequence

Protein

• Length: 1166 a.a.
• Molecular weight: 134645.65 Da
• Isoelectric Point: 5.4734

>NTDB_id=713217 NP434_RS05770 WP_256683338.1 1106670..1110170(+) (addB) [Bacillus subtilis subsp. subtilis strain BGSC 10A5]
MGAEFLVGRSGSGKTKLIIDSIQDELRRAPFGKPIIFLVPDQMTFLMEYELAKTPDMGGMIRAQVFSFSRLAWRILQHTG
GMSRPFLTSTGVQMLLRKLIEEHKQEFKVYQKASDKSGFTAQVERMLTEFKRYCLEPEDIRRMAESGTASEYRGERVLSE
KLHDLSILYQQMEKSLADQYLHSEDYLTLLAEHIPLAEDIKGAHIYVDGFYQFTPQEFRVLEQLMVHAEHITFSLTADKP
SYEREPHELELFRMTGKTYYRLHQKAKELNLDITYKELSGTERHTKTPELAHLEAQYEARPAVPYAEKQEALTVMQAANR
RAELEGIAREIHALVREKGYRYKDVAILARQPEDYKDMVKEVFADYEIPYFIDGKASMLNHPLIEFIRSSLDVLKGNWRY
EAVFRCVKTELLFPLNEPKAKVREQVDQLENYCIAYGIKGDRWTKGDRFQYRRFVSLDDDFAQTDQEIEMENMLNDTRDW
IVPPLFQLQKRMKKAKTVQEKAEALYRYLEETDVPLKLDQERQRAEDDGRIIEAQQHQQAWDAVIQLLEEFVEMMGDDEI
SLDLFQQMIEAGAESLTFSLIPPALDQVFVGNMDLSRMYGTSCTFVLGANDGVLPARPDENGVLSDDDREWLKTIGVELS
SGGRERLLEEHFLIYMAFSSPSDRLYVSYPIADAEGKTLLPSMIVKRLEELFPHHKERLLTNEPEQVSDEEQLMYVVNKS
VAQSFTASQLRLWTREYDISDVWWSTYNVLMSEQDRLQSKKLFSSLFFRNEVKQLERSVSRQLYGERIQGSVSRMETFNA
CPFSHFASHGLHLKERQFFKLEAPDIGQLFHSSLKLISDRLREQKLDWRDLTKEQCELFSYDAVERLAPKLQKEILLSSN
RHYYVKEKLQKIVTRVSGILSEHAKASGFVPIGLELGFGGKGPLPPLTFQLKNGCTMELVGRIDRVDKAESSKGLLLRVV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSADWLGMRATPAGVLYFHIHDPMIQSNLPLGLDEIEQEIFKKFKMKGL
LLGDQEVVRLMDTTLQEGRSNIINAGLKKDGSLRSDSAAVGEKEFDLLTKHVRRTFQEAGEQITDGRVSIEPYKMKNKTP
CTYCAFKSVCQFDESLEENEYRPLKAEKDKTILEWIKKEADGNEHS

Nucleotide

• Length: 3501 bp

>NTDB_id=713217 NP434_RS05770 WP_256683338.1 1106670..1110170(+) (addB) [Bacillus subtilis subsp. subtilis strain BGSC 10A5]
TTGGGAGCAGAGTTTTTAGTAGGCAGGTCCGGGAGTGGAAAAACGAAGCTGATCATCGACAGCATTCAGGATGAATTGCG
CCGGGCTCCATTCGGGAAGCCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATACGAGCTTGCTAAAA
CGCCAGATATGGGCGGGATGATACGCGCTCAAGTATTCAGTTTTTCACGATTGGCCTGGCGCATCCTCCAGCATACGGGA
GGAATGAGCAGGCCGTTTCTGACGAGCACCGGCGTACAAATGCTCCTGCGGAAGCTCATTGAGGAGCATAAACAGGAGTT
CAAAGTCTATCAAAAAGCGAGTGACAAAAGCGGGTTTACCGCACAGGTTGAGCGGATGCTGACAGAGTTTAAGCGCTATT
GTCTGGAACCGGAGGATATCCGCCGGATGGCGGAAAGCGGAACAGCTTCCGAGTATCGCGGAGAACGTGTTTTATCTGAA
AAGCTCCATGACTTATCAATTCTGTATCAGCAAATGGAAAAAAGCCTCGCAGACCAATATCTTCACTCTGAGGATTATTT
GACATTGCTGGCAGAGCATATCCCGCTTGCGGAAGATATAAAAGGCGCCCATATCTATGTGGATGGCTTTTATCAGTTTA
CCCCGCAGGAATTCAGGGTGTTGGAGCAGCTTATGGTTCATGCGGAGCATATCACGTTTTCGCTCACAGCGGACAAGCCG
TCATATGAGCGGGAGCCGCATGAACTGGAATTGTTCAGAATGACGGGGAAAACCTATTACCGCCTGCATCAGAAGGCGAA
GGAACTGAATTTGGACATTACGTATAAAGAGCTGAGCGGGACTGAGCGGCATACAAAGACGCCGGAATTGGCGCATCTAG
AGGCACAGTATGAAGCGCGTCCGGCCGTTCCATACGCAGAAAAACAAGAAGCCCTTACTGTGATGCAGGCCGCAAACAGA
CGAGCTGAGCTGGAAGGCATTGCTCGGGAAATTCACGCCCTAGTCAGAGAGAAGGGATATCGCTATAAGGATGTAGCAAT
TCTTGCGCGCCAGCCGGAAGACTACAAGGATATGGTGAAGGAAGTTTTCGCAGATTACGAGATTCCTTATTTCATTGACG
GAAAAGCATCTATGCTGAACCATCCGTTAATTGAATTTATCCGGTCAAGCCTTGATGTTCTGAAAGGGAATTGGCGTTAT
GAAGCGGTGTTTCGCTGCGTGAAAACCGAACTGCTATTCCCGCTCAATGAACCGAAGGCCAAAGTGAGAGAACAGGTCGA
TCAGCTCGAAAATTACTGTATCGCCTATGGTATTAAAGGCGACCGCTGGACAAAGGGCGATCGGTTCCAATACAGGCGTT
TTGTGTCATTGGATGATGATTTTGCGCAGACTGATCAGGAAATCGAAATGGAAAACATGTTGAATGACACCCGCGATTGG
ATTGTTCCGCCGCTTTTTCAGCTTCAAAAACGCATGAAAAAAGCGAAGACGGTTCAAGAGAAGGCGGAGGCGCTCTATCG
TTATTTAGAAGAGACGGATGTGCCGCTGAAGCTGGATCAGGAAAGGCAGCGTGCTGAGGATGACGGCAGAATCATTGAAG
CGCAGCAGCATCAGCAGGCGTGGGACGCAGTCATTCAGCTGCTTGAGGAGTTTGTTGAAATGATGGGAGATGATGAGATT
TCTCTTGATTTGTTTCAGCAAATGATAGAAGCCGGCGCGGAGTCGCTTACGTTTTCTCTAATTCCGCCTGCGCTTGACCA
GGTGTTTGTCGGCAATATGGATTTGTCCAGAATGTATGGCACCTCCTGCACCTTTGTGCTCGGGGCAAACGACGGCGTTC
TGCCGGCACGCCCTGATGAAAACGGGGTCCTGTCGGATGATGACCGGGAATGGCTGAAAACGATTGGGGTTGAGCTATCC
TCAGGCGGACGGGAGCGTTTGCTTGAAGAGCACTTCCTCATCTACATGGCGTTTTCAAGTCCGTCTGACCGGCTTTACGT
ATCGTATCCGATTGCTGATGCGGAAGGAAAAACGCTTTTGCCGTCGATGATCGTTAAGCGGCTGGAAGAACTGTTTCCGC
ATCATAAGGAGCGCCTGTTAACAAATGAACCTGAACAGGTCAGCGATGAGGAACAGCTGATGTATGTCGTGAATAAAAGC
GTGGCACAGTCCTTTACCGCGAGCCAGCTCAGGTTATGGACACGGGAATACGACATCAGCGACGTCTGGTGGAGCACGTA
CAATGTGCTGATGAGTGAGCAGGACAGGCTGCAATCGAAAAAGCTGTTCTCAAGCCTGTTTTTCCGGAATGAAGTGAAGC
AGCTTGAACGCAGCGTGTCGAGACAGCTCTATGGTGAACGCATTCAGGGCAGTGTATCGAGAATGGAAACCTTTAACGCA
TGCCCGTTTTCCCATTTTGCGTCACACGGGCTGCATTTGAAGGAACGGCAATTCTTCAAGCTTGAAGCACCGGATATCGG
CCAGCTGTTTCATTCCAGCTTAAAGCTGATTTCGGACAGGCTGCGTGAGCAAAAGCTGGATTGGCGCGATTTAACGAAGG
AGCAGTGCGAGCTGTTTTCCTATGATGCGGTAGAACGGCTGGCACCGAAACTGCAAAAGGAAATTCTGCTCAGCTCAAAC
CGGCATTATTATGTGAAGGAAAAACTGCAAAAAATTGTGACACGTGTGTCCGGCATTTTAAGCGAGCATGCGAAAGCGAG
CGGATTCGTACCGATCGGGCTTGAACTGGGCTTTGGAGGAAAAGGGCCGCTTCCGCCGCTGACCTTTCAACTGAAAAACG
GCTGTACGATGGAACTCGTCGGGCGAATTGACCGTGTTGATAAGGCTGAAAGCTCAAAAGGCCTGCTCCTCAGGGTTGTC
GATTATAAATCAAGCGACAAAGGCCTTGACTTAGCGGAAGTATATTACGGATTGGCACTGCAAATGCTGACGTACCTTGA
TTTATCGATTACACATTCAGCTGACTGGCTCGGGATGAGGGCGACGCCTGCCGGAGTGCTGTATTTCCATATTCATGACC
CGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTG
CTCCTCGGTGATCAGGAAGTGGTTCGCCTCATGGATACAACCCTTCAAGAGGGACGGTCAAATATCATAAACGCCGGCTT
GAAAAAAGACGGATCTCTTAGATCAGACTCGGCAGCAGTCGGTGAAAAGGAATTTGATCTTTTGACAAAGCATGTGCGCC
GCACCTTCCAGGAAGCGGGCGAACAAATCACCGACGGGCGCGTATCCATTGAGCCGTACAAAATGAAGAACAAGACGCCG
TGCACATACTGTGCGTTCAAATCAGTATGCCAATTTGATGAATCACTGGAAGAAAACGAGTATCGCCCATTAAAGGCTGA
AAAGGACAAGACAATACTTGAGTGGATAAAAAAGGAGGCGGATGGCAATGAACATTCCTAA

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (305-669)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (790-1131)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addB	Bacillus subtilis subsp. subtilis str. 168	99.571	100	0.996