Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   NP431_RS13325 Genome accession   NZ_CP101905
Coordinates   2899193..2900209 (-) Length   338 a.a.
NCBI ID   WP_020334617.1    Uniprot ID   A0AAN0Y4M4
Organism   Vibrio natriegens strain 14048     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2894193..2905209
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NP431_RS13300 (NP431_13300) aroK 2895065..2895583 (-) 519 WP_014233147.1 shikimate kinase AroK -
  NP431_RS13305 (NP431_13305) pilQ 2895772..2897475 (-) 1704 WP_020334613.1 type IV pilus secretin PilQ Machinery gene
  NP431_RS13310 (NP431_13310) pilP 2897543..2898058 (-) 516 WP_020334614.1 pilus assembly protein PilP Machinery gene
  NP431_RS13315 (NP431_13315) pilO 2898048..2898641 (-) 594 WP_020334615.1 type 4a pilus biogenesis protein PilO Machinery gene
  NP431_RS13320 (NP431_13320) pilN 2898634..2899209 (-) 576 WP_024372660.1 PilN domain-containing protein Machinery gene
  NP431_RS13325 (NP431_13325) pilM 2899193..2900209 (-) 1017 WP_020334617.1 type IV pilus assembly protein PilM Machinery gene
  NP431_RS13330 (NP431_13330) - 2900353..2902920 (+) 2568 WP_020334618.1 penicillin-binding protein 1A -
  NP431_RS13335 (NP431_13335) oxyR 2902984..2903892 (-) 909 WP_014233154.1 DNA-binding transcriptional regulator OxyR -
  NP431_RS13340 (NP431_13340) - 2904051..2904779 (+) 729 WP_020334619.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37587.78 Da        Isoelectric Point: 7.2098

>NTDB_id=712654 NP431_RS13325 WP_020334617.1 2899193..2900209(-) (pilM) [Vibrio natriegens strain 14048]
MDKRIVTGIDIGHNSLKAVVLKPNGEQFALLGYKEILLKDGIVAENNTINHQEIVNTLKQVKKDLPFGASRVAISVPDNA
VISKKLHIEQDLEENEIEFAVVQAFSHQSPFPVDELSLDFVRLNHEGGLRGPVSYQVFATRKDVVESRVEAIRKSGFKPV
LVEVHSQSLGQIWKLATERFPDKSQYGLLDVGSVSSSFTMFTEQNELFHKEFACGTGSIQCSVQESPLSGEKQAKTSQFN
RQMVDRVKRQIQLYTSINGSQNIKGLWLSGEGSTTPMLAEELSHQLAVECELLNPLGLFEMNVAKRKRRAVEWQNFSTAA
GLAVRGIQWLGGHRATSN

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=712654 NP431_RS13325 WP_020334617.1 2899193..2900209(-) (pilM) [Vibrio natriegens strain 14048]
ATGGATAAGCGAATCGTTACAGGCATCGATATAGGCCATAACAGCCTCAAAGCCGTCGTACTTAAACCCAATGGCGAGCA
ATTCGCCTTGTTGGGTTACAAAGAAATATTACTTAAGGATGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAATACCCTTAAACAGGTGAAAAAAGACCTGCCATTTGGTGCAAGCAGAGTGGCAATTTCAGTGCCTGATAATGCG
GTTATCAGCAAAAAACTGCACATCGAACAAGACCTTGAAGAGAACGAAATAGAGTTCGCGGTCGTGCAGGCATTTTCTCA
TCAATCTCCTTTCCCCGTAGATGAGTTGAGTCTGGACTTTGTCCGTCTTAATCATGAAGGAGGCCTGCGAGGGCCAGTTA
GCTACCAAGTTTTTGCAACACGTAAAGATGTCGTGGAGAGTCGGGTTGAAGCAATACGTAAATCCGGTTTTAAACCTGTC
CTCGTCGAGGTCCATTCACAAAGCCTCGGGCAGATATGGAAACTGGCCACAGAACGTTTTCCAGATAAAAGTCAGTACGG
TTTGCTTGATGTTGGTTCTGTGTCGAGCTCTTTCACGATGTTCACCGAGCAAAACGAGCTATTTCATAAAGAATTTGCCT
GTGGAACAGGTTCCATTCAGTGCTCTGTGCAGGAAAGTCCTCTGTCCGGAGAAAAGCAAGCCAAAACGAGTCAATTTAAC
CGTCAGATGGTCGATCGTGTCAAACGTCAGATACAGCTTTATACGTCGATTAATGGGTCACAAAACATCAAAGGTCTTTG
GTTATCCGGTGAAGGGTCAACAACGCCAATGTTGGCTGAGGAGTTATCTCATCAATTGGCGGTCGAGTGCGAGTTACTGA
ATCCTTTAGGCCTATTTGAAATGAACGTCGCTAAACGTAAGCGTCGAGCGGTGGAATGGCAGAATTTTTCAACCGCCGCT
GGCTTGGCAGTACGAGGTATCCAATGGTTGGGAGGTCATCGTGCTACATCGAATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

74.556

100

0.746
  pilM Vibrio cholerae strain A1552

48.521

100

0.485