Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   NPA43_RS12085 Genome accession   NZ_CP101833
Coordinates   2367190..2369514 (-) Length   774 a.a.
NCBI ID   WP_256498843.1    Uniprot ID   -
Organism   Bacillus pumilus strain NDY-10     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2362190..2374514
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NPA43_RS12070 (NPA43_12070) dtd 2363840..2364283 (-) 444 WP_099727760.1 D-aminoacyl-tRNA deacylase -
  NPA43_RS12075 (NPA43_12075) - 2364302..2366512 (-) 2211 WP_099727761.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  NPA43_RS12080 (NPA43_12080) - 2366654..2367166 (-) 513 WP_034321337.1 adenine phosphoribosyltransferase -
  NPA43_RS12085 (NPA43_12085) recJ 2367190..2369514 (-) 2325 WP_256498843.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  NPA43_RS12090 (NPA43_12090) - 2369596..2369952 (-) 357 WP_256498844.1 lipopolysaccharide assembly LapA domain-containing protein -
  NPA43_RS12095 (NPA43_12095) - 2370054..2370542 (-) 489 WP_099727764.1 cation:proton antiporter regulatory subunit -
  NPA43_RS12100 (NPA43_12100) secDF 2370790..2373018 (-) 2229 WP_256498845.1 protein translocase subunit SecDF -
  NPA43_RS12105 (NPA43_12105) - 2373060..2373356 (-) 297 WP_003216788.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 774 a.a.        Molecular weight: 87405.19 Da        Isoelectric Point: 5.1421

>NTDB_id=712242 NPA43_RS12085 WP_256498843.1 2367190..2369514(-) (recJ) [Bacillus pumilus strain NDY-10]
MLLSKMRWEYEHPSEEKVKSLSENLNISALTASLLVKRGLEEVEEARSFLFDQKAEFHDPFLLKGMKEAVERINKAIEEQ
ESIVIFGDYDADGVTSTSVLLHTLKERSAKVDFYIPDRFKEGYGPNEQAFRYIKEQGASLVITVDTGIAAVNEARIAKEI
GLDLIITDHHEPSDELPDALAIVHPKQPGCEYPFKELAGVGVAFKVAHALLDKLPVHLLDLAAIGTIADLVPLHDENRWL
AKKGLVQLRQSNRPGLKALMKEAGATLEEANEETVGFQIAPRLNAVGRIEQADPAVHLLMTEDMEEAEELARFVQELNKE
RQKIVSTITEEAIQMVEESGGDQSAIVVAKAGWNPGVVGIVASKLTDTFSRPAIVLGIDEDTQMAKGSARSIPGFDLFFH
LSKCRDILPHFGGHPMAAGMTLRADDVPLLRERLNQYANETLTEEDFIPIQRVDAVCKVDEITVQAIEEVGMLSPFGMHN
PKPFIMIEDAKLEDIRTIGANHHHIKMSLKDEDKLLDCVGFHQGKLKEELVPGSRISVIGEMSINEWNNRKKPQLMLKDA
RVDEWQLFDLRGKKDWEKKVSTLDPEKYLVFCFDEETKHEVNIPVHHVHAENVSTFDVQDKYVVFADVPADEHLLKLLLE
NQKPARIYTVFQRKEDHFMSSFPSRDQFKWFYGFLLKRGNFPLKEQGMELAKHRGWTKDTIIFMTKVFFELGFVKIENGV
LSIVVDAPKKDLTASASYTAKQRLMELDQTLTYSSAKELKEWLNSMMSEVSPVL

Nucleotide


Download         Length: 2325 bp        

>NTDB_id=712242 NPA43_RS12085 WP_256498843.1 2367190..2369514(-) (recJ) [Bacillus pumilus strain NDY-10]
ATGTTATTATCCAAAATGCGCTGGGAATATGAACATCCAAGCGAAGAAAAAGTGAAATCACTCTCTGAAAATCTGAATAT
TTCAGCGCTGACTGCATCGCTTCTTGTAAAAAGAGGACTCGAAGAAGTAGAAGAAGCGAGGTCTTTTTTATTTGATCAAA
AAGCCGAATTTCATGATCCATTCCTATTAAAAGGAATGAAAGAAGCAGTAGAGCGCATCAACAAAGCGATCGAAGAGCAG
GAATCGATTGTGATCTTCGGAGACTATGATGCGGATGGTGTGACAAGTACGTCTGTGCTTTTACATACATTAAAAGAGCG
ATCTGCAAAGGTAGATTTTTATATTCCAGATCGATTTAAAGAAGGCTATGGGCCTAACGAACAGGCTTTTCGATATATCA
AAGAGCAAGGAGCATCGCTTGTCATCACGGTCGATACAGGCATTGCTGCTGTGAATGAAGCACGTATTGCAAAAGAAATA
GGATTAGACCTGATCATCACAGATCACCATGAACCAAGTGATGAGCTGCCTGATGCGCTTGCGATCGTACACCCTAAACA
GCCGGGCTGTGAGTATCCATTTAAAGAACTCGCCGGTGTCGGTGTTGCGTTTAAAGTGGCGCATGCACTCCTTGATAAGC
TGCCAGTTCATCTATTAGATCTTGCTGCGATTGGGACAATTGCCGATCTTGTCCCGCTGCATGATGAAAATAGATGGCTC
GCAAAAAAGGGACTCGTGCAGCTTCGCCAGTCTAACAGACCGGGGCTAAAAGCGTTAATGAAAGAAGCTGGAGCCACTCT
CGAAGAAGCAAACGAGGAAACGGTCGGCTTTCAAATTGCTCCAAGGCTGAATGCTGTAGGGCGAATTGAGCAGGCAGATC
CGGCTGTTCATCTTCTGATGACAGAGGATATGGAGGAAGCAGAAGAGCTCGCTCGTTTTGTACAAGAACTCAACAAAGAA
CGGCAGAAAATTGTGAGTACGATCACGGAAGAAGCCATTCAAATGGTCGAAGAATCAGGCGGTGACCAGTCTGCCATCGT
CGTAGCCAAAGCTGGCTGGAATCCAGGGGTTGTCGGCATTGTTGCCTCAAAATTGACGGATACATTTTCTCGTCCGGCGA
TTGTGCTTGGGATAGATGAAGACACGCAAATGGCAAAAGGATCAGCGCGGAGTATTCCAGGCTTTGATCTCTTTTTCCAT
TTGAGCAAATGCCGCGATATCCTGCCGCATTTCGGCGGGCACCCTATGGCAGCAGGTATGACACTTCGAGCGGATGATGT
TCCTTTACTGAGGGAAAGATTGAATCAATATGCAAATGAAACGTTGACAGAAGAAGATTTTATTCCGATTCAGCGGGTCG
ATGCAGTGTGCAAAGTAGACGAAATTACGGTTCAAGCAATTGAAGAAGTGGGGATGCTGTCACCATTTGGCATGCACAAT
CCAAAGCCGTTCATTATGATTGAGGATGCCAAGCTTGAAGACATCCGAACGATTGGGGCAAATCATCATCACATTAAAAT
GTCTCTGAAAGACGAAGACAAGCTGCTTGACTGTGTCGGTTTTCACCAAGGTAAATTAAAAGAAGAGCTCGTCCCTGGAA
GCCGAATTTCCGTGATTGGCGAAATGTCAATTAATGAATGGAATAACCGGAAGAAACCTCAGCTCATGCTAAAAGATGCC
CGAGTAGATGAATGGCAGCTGTTTGATTTACGCGGGAAAAAGGATTGGGAAAAGAAAGTTTCGACGCTTGATCCAGAAAA
ATATCTTGTTTTCTGTTTTGATGAAGAAACTAAACATGAAGTCAATATCCCGGTTCATCATGTACATGCAGAAAATGTTT
CAACGTTTGATGTGCAAGACAAGTATGTGGTCTTTGCGGATGTTCCCGCAGATGAGCACTTGCTTAAGCTGCTTTTAGAA
AATCAAAAGCCAGCCAGAATTTATACGGTCTTTCAAAGAAAAGAAGATCACTTTATGTCTTCTTTCCCTAGTCGTGACCA
ATTCAAATGGTTTTATGGGTTCTTGTTAAAACGGGGAAATTTTCCTCTCAAAGAACAAGGGATGGAGCTTGCCAAGCATA
GAGGTTGGACAAAAGATACAATCATTTTTATGACAAAGGTGTTTTTTGAACTCGGTTTTGTTAAAATAGAGAATGGTGTG
CTGTCAATAGTAGTTGATGCCCCGAAAAAAGATTTAACCGCATCAGCGTCCTATACAGCAAAACAACGATTGATGGAACT
CGATCAAACACTAACATATTCCTCAGCCAAAGAGCTGAAGGAATGGCTGAACAGCATGATGAGCGAAGTCTCACCGGTGC
TTTGA

Domains

Predicted by InterproScan.

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-763)

DHHA1 domain (DHHA1)

(314-437)

RecJ OB domain (RecJ_OB)

(453-559)

DHH family (DHH)

(83-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

67.31

100

0.676