Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   PMJ91_RS27735 Genome accession   NZ_CP116715
Coordinates   5805381..5805806 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa strain 2856     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5800381..5810806
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PMJ91_RS27720 (PMJ91_27720) pilX 5800945..5801532 (+) 588 WP_015648054.1 type 4a pilus minor pilin PilX -
  PMJ91_RS27725 (PMJ91_27725) pilY1 5801544..5805035 (+) 3492 WP_015648053.1 type 4a pilus biogenesis protein PilY1 -
  PMJ91_RS27730 (PMJ91_27730) pilY2 5805037..5805384 (+) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  PMJ91_RS27735 (PMJ91_27735) comF 5805381..5805806 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  PMJ91_RS27740 (PMJ91_27740) ispH 5805853..5806797 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  PMJ91_RS27745 (PMJ91_27745) fkpB 5806883..5807323 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  PMJ91_RS27750 (PMJ91_27750) lspA 5807316..5807825 (-) 510 WP_003102615.1 signal peptidase II -
  PMJ91_RS27755 (PMJ91_27755) ileS 5807818..5810649 (-) 2832 WP_004352756.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=712033 PMJ91_RS27735 WP_003094721.1 5805381..5805806(+) (comF) [Pseudomonas aeruginosa strain 2856]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=712033 PMJ91_RS27735 WP_003094721.1 5805381..5805806(+) (comF) [Pseudomonas aeruginosa strain 2856]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGGCAGGCGCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383