Detailed information    

insolico Bioinformatically predicted

Overview

Name   mutS/mutS2   Type   Machinery gene
Locus tag   POF64_RS08775 Genome accession   NZ_CP116713
Coordinates   1715069..1717408 (-) Length   779 a.a.
NCBI ID   WP_001060323.1    Uniprot ID   Q3JZH6
Organism   Streptococcus agalactiae strain A909 Cas9 AEKOrevertant     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1710069..1722408
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  POF64_RS08745 (POF64_08745) ssbA 1710346..1710837 (-) 492 WP_000609585.1 single-stranded DNA-binding protein Machinery gene
  POF64_RS08750 (POF64_08750) rpsF 1710849..1711136 (-) 288 WP_001151773.1 30S ribosomal protein S6 -
  POF64_RS08755 (POF64_08755) mutY 1712200..1713354 (+) 1155 WP_000566235.1 A/G-specific adenine glycosylase -
  POF64_RS08760 (POF64_08760) - 1713531..1714124 (+) 594 WP_000402394.1 helix-turn-helix transcriptional regulator -
  POF64_RS08765 (POF64_08765) trxA 1714170..1714502 (-) 333 WP_001932060.1 thioredoxin -
  POF64_RS08770 (POF64_08770) - 1714565..1715065 (-) 501 WP_000446812.1 phosphatase PAP2 family protein -
  POF64_RS08775 (POF64_08775) mutS/mutS2 1715069..1717408 (-) 2340 WP_001060323.1 endonuclease MutS2 Machinery gene
  POF64_RS08780 (POF64_08780) - 1717493..1718035 (-) 543 WP_000949954.1 CvpA family protein -
  POF64_RS08785 (POF64_08785) zapA 1718038..1718349 (-) 312 WP_000448289.1 cell division protein ZapA -
  POF64_RS08790 (POF64_08790) rnhC 1718460..1719353 (+) 894 WP_001092527.1 ribonuclease HIII -
  POF64_RS08795 (POF64_08795) lepB 1719369..1719962 (+) 594 WP_000657514.1 signal peptidase I -

Sequence

Protein


Download         Length: 779 a.a.        Molecular weight: 87740.80 Da        Isoelectric Point: 6.2259

>NTDB_id=711822 POF64_RS08775 WP_001060323.1 1715069..1717408(-) (mutS/mutS2) [Streptococcus agalactiae strain A909 Cas9 AEKOrevertant]
MNNKILEQLEFNKVKELILPYLKTEQSQEELSELEPMTEAPKIEKSFNEISDMEQIFVEHHSFGIVSLSSISESLKRLEL
SADLNIQELLAIKKVLQSSSDMIHFYSDLDNVSFQSLDRLFENLEQFPNLQGSFQAINDGGFLEHFASPELERIRRQLTN
SERRVRQILQDMLKEKAELLSENLIASRSGRSVLPVKNTYRNRISGVVHDISSSGSTVYIEPRAVVTLNEEITQLRADER
HEESRILHAFSDLLRPHVATIRNNAWILGHLDFVRAKYLFMSDNKATIPEISNDSTLALINVRHPLLSNPVANDLHFDQD
LTAIVITGPNTGGKTIMLKTLGLAQLMGQSGLPVLADKGSKIAVFNNIFADIGDEQSIEQSLSTFSSHMTHIVSILNEAD
HNSLVLFDELGAGTDPQEGASLAMAILEHLRLSNIKTMATTHYPELKAYGIETNFVENASMEFDAETLSPTYRFMQGVPG
RSNAFEIASRLGLAPFIVKQAKQMTDSDSDVNRIIEQLEAQTLETRRRLDHIKEVEQENLKFNRAVKKLYNEFSHERDKE
LEKIYQEAQEIVDMALNESDTILKKLNDKSQLKPHEIIDAKAQIKKLAPQVDLSKNKVLNKAKKIKAARAPRIGDDIIVT
SYGQRGTLTSQLKDGRWEAQVGIIKMTLTQDEFTLVRVQEEQKVKSKQINVVKKADSSGPRARLDLRGKRYEEAMQELDN
FIDQALLNNMGQVDIIHGIGTGVIREGVTKYLRRNKHVKHFAYAPQNAGGSGATIVTLG

Nucleotide


Download         Length: 2340 bp        

>NTDB_id=711822 POF64_RS08775 WP_001060323.1 1715069..1717408(-) (mutS/mutS2) [Streptococcus agalactiae strain A909 Cas9 AEKOrevertant]
ATGAATAACAAGATTTTAGAACAGTTAGAATTTAACAAAGTTAAGGAATTGATATTACCTTATCTCAAGACAGAACAATC
ACAAGAAGAATTATCAGAGCTGGAGCCGATGACGGAGGCTCCTAAAATAGAAAAAAGTTTTAATGAAATTTCTGACATGG
AACAGATTTTTGTTGAACATCACTCATTTGGCATAGTCAGCCTAAGTTCAATCTCTGAGAGTTTAAAACGCTTAGAGCTT
TCAGCTGATCTTAATATTCAAGAACTTTTGGCTATCAAAAAAGTTTTACAGAGTTCTTCGGATATGATTCACTTTTATTC
TGATTTGGATAATGTTTCTTTCCAATCTTTGGATCGTTTGTTTGAAAATTTGGAACAATTCCCTAATCTGCAAGGGTCTT
TTCAAGCTATCAATGATGGTGGTTTTTTAGAACATTTTGCGAGTCCAGAATTAGAGCGTATCCGTCGTCAATTAACAAAC
AGTGAACGACGGGTTCGTCAGATTTTACAGGATATGCTTAAGGAAAAAGCAGAGCTTTTATCAGAGAATCTAATCGCTAG
TCGTAGTGGACGAAGTGTCCTACCAGTAAAAAATACTTATCGGAATCGTATTTCTGGTGTGGTTCATGACATCTCTTCTT
CAGGAAGTACTGTTTATATTGAGCCTCGTGCTGTAGTTACACTAAACGAAGAGATAACGCAGCTTAGAGCTGACGAACGT
CATGAAGAAAGTCGTATTTTACACGCATTTTCAGACTTGTTAAGACCCCATGTCGCCACTATTAGAAATAATGCATGGAT
TCTTGGGCATCTTGATTTTGTAAGGGCTAAATATCTTTTTATGTCTGATAATAAGGCGACGATACCTGAGATTTCTAATG
ACAGCACGTTAGCATTAATCAATGTTCGTCATCCTCTGTTAAGTAACCCTGTGGCTAATGACTTACATTTTGATCAAGAT
TTAACTGCAATTGTCATCACTGGTCCCAATACTGGTGGTAAGACGATTATGCTAAAAACACTCGGTTTAGCACAATTAAT
GGGACAGTCTGGTTTGCCAGTATTAGCGGATAAAGGTAGTAAAATTGCAGTATTTAACAATATCTTTGCAGATATTGGCG
ATGAGCAATCTATTGAACAAAGTCTATCAACTTTTTCTAGTCATATGACGCACATAGTCAGTATTTTAAACGAGGCTGAC
CACAATAGTTTAGTCCTCTTTGATGAACTGGGAGCAGGAACGGATCCTCAAGAAGGTGCTAGTTTGGCTATGGCTATTTT
AGAACACCTTAGGTTAAGTAATATCAAAACGATGGCGACCACGCACTATCCAGAATTAAAAGCTTATGGGATTGAGACAA
ATTTTGTAGAGAATGCGAGCATGGAATTTGATGCCGAAACGCTTAGCCCTACGTATCGCTTTATGCAAGGAGTTCCTGGA
CGATCAAATGCATTTGAAATTGCTTCTCGCCTTGGTTTAGCTCCATTTATTGTTAAACAAGCTAAGCAGATGACAGATTC
TGACTCAGATGTTAACCGTATTATTGAACAGTTAGAGGCACAGACACTTGAGACACGTAGAAGACTGGATCATATTAAAG
AAGTTGAACAAGAAAACCTCAAATTCAATCGTGCGGTTAAGAAACTCTATAATGAATTTTCACATGAGCGCGATAAAGAG
TTAGAAAAAATCTATCAAGAAGCTCAAGAAATTGTAGATATGGCTTTGAATGAGAGTGATACTATCTTAAAAAAACTCAA
TGATAAGAGCCAATTAAAACCTCACGAAATTATAGATGCTAAGGCACAAATAAAAAAATTAGCACCTCAAGTTGATTTAT
CAAAAAATAAAGTCTTAAATAAGGCTAAAAAAATTAAAGCAGCTCGTGCTCCTAGAATTGGTGATGATATTATAGTGACT
AGCTATGGACAGCGAGGTACCTTAACTAGTCAATTAAAAGATGGACGTTGGGAAGCACAAGTGGGAATTATCAAAATGAC
ATTAACACAAGATGAATTTACCCTTGTTAGAGTCCAAGAAGAACAGAAAGTCAAAAGTAAACAGATTAATGTGGTTAAAA
AGGCTGATAGTTCTGGACCAAGAGCTCGACTTGATCTTAGAGGTAAAAGATACGAAGAAGCTATGCAAGAGTTAGATAAT
TTTATTGATCAAGCATTGCTTAACAATATGGGACAAGTTGATATCATTCATGGTATTGGTACAGGCGTTATCCGTGAGGG
AGTGACAAAATATCTTCGTCGTAATAAGCACGTTAAGCATTTTGCTTATGCCCCACAAAATGCAGGGGGATCTGGCGCCA
CAATTGTAACGTTAGGGTAA

Domains

Predicted by InterProScan.

MutS domain V (MutS_V)

(324-505)

MutS2 and Smr-associated SH3 domain (MSSS)

(633-669)

Smr domain (Smr)

(704-778)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3JZH6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  mutS/mutS2 Bacillus subtilis subsp. subtilis str. 168

40.506

100

0.411